AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

Though AsCas12a fills a crucial gap in the current genome editing toolbox, it exhibits relatively poor editing efficiency, restricting its overall utility. Here we isolate an engineered variant, “AsCas12a Ultra”, that increased editing efficiency to nearly 100% at all sites examined in HSPCs, iPSCs,...

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Detalles Bibliográficos
Autores principales: Zhang, Liyang, Zuris, John A., Viswanathan, Ramya, Edelstein, Jasmine N., Turk, Rolf, Thommandru, Bernice, Rube, H. Tomas, Glenn, Steve E., Collingwood, Michael A., Bode, Nicole M., Beaudoin, Sarah F., Lele, Swarali, Scott, Sean N., Wasko, Kevin M., Sexton, Steven, Borges, Christopher M., Schubert, Mollie S., Kurgan, Gavin L., McNeill, Matthew S., Fernandez, Cecilia A., Myer, Vic E., Morgan, Richard A., Behlke, Mark A., Vakulskas, Christopher A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222333/
https://www.ncbi.nlm.nih.gov/pubmed/34162850
http://dx.doi.org/10.1038/s41467-021-24017-8

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