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Single-component near-infrared optogenetic systems for gene transcription regulation
Near-infrared (NIR) optogenetic systems for transcription regulation are in high demand because NIR light exhibits low phototoxicity, low scattering, and allows combining with probes of visible range. However, available NIR optogenetic systems consist of several protein components of large size and...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222386/ https://www.ncbi.nlm.nih.gov/pubmed/34162879 http://dx.doi.org/10.1038/s41467-021-24212-7 |
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author | Kaberniuk, Andrii A. Baloban, Mikhail Monakhov, Mikhail V. Shcherbakova, Daria M. Verkhusha, Vladislav V. |
author_facet | Kaberniuk, Andrii A. Baloban, Mikhail Monakhov, Mikhail V. Shcherbakova, Daria M. Verkhusha, Vladislav V. |
author_sort | Kaberniuk, Andrii A. |
collection | PubMed |
description | Near-infrared (NIR) optogenetic systems for transcription regulation are in high demand because NIR light exhibits low phototoxicity, low scattering, and allows combining with probes of visible range. However, available NIR optogenetic systems consist of several protein components of large size and multidomain structure. Here, we engineer single-component NIR systems consisting of evolved photosensory core module of Idiomarina sp. bacterial phytochrome, named iLight, which are smaller and packable in adeno-associated virus. We characterize iLight in vitro and in gene transcription repression in bacterial and gene transcription activation in mammalian cells. Bacterial iLight system shows 115-fold repression of protein production. Comparing to multi-component NIR systems, mammalian iLight system exhibits higher activation of 65-fold in cells and faster 6-fold activation in deep tissues of mice. Neurons transduced with viral-encoded iLight system exhibit 50-fold induction of fluorescent reporter. NIR light-induced neuronal expression of green-light-activatable CheRiff channelrhodopsin causes 20-fold increase of photocurrent and demonstrates efficient spectral multiplexing. |
format | Online Article Text |
id | pubmed-8222386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-82223862021-07-09 Single-component near-infrared optogenetic systems for gene transcription regulation Kaberniuk, Andrii A. Baloban, Mikhail Monakhov, Mikhail V. Shcherbakova, Daria M. Verkhusha, Vladislav V. Nat Commun Article Near-infrared (NIR) optogenetic systems for transcription regulation are in high demand because NIR light exhibits low phototoxicity, low scattering, and allows combining with probes of visible range. However, available NIR optogenetic systems consist of several protein components of large size and multidomain structure. Here, we engineer single-component NIR systems consisting of evolved photosensory core module of Idiomarina sp. bacterial phytochrome, named iLight, which are smaller and packable in adeno-associated virus. We characterize iLight in vitro and in gene transcription repression in bacterial and gene transcription activation in mammalian cells. Bacterial iLight system shows 115-fold repression of protein production. Comparing to multi-component NIR systems, mammalian iLight system exhibits higher activation of 65-fold in cells and faster 6-fold activation in deep tissues of mice. Neurons transduced with viral-encoded iLight system exhibit 50-fold induction of fluorescent reporter. NIR light-induced neuronal expression of green-light-activatable CheRiff channelrhodopsin causes 20-fold increase of photocurrent and demonstrates efficient spectral multiplexing. Nature Publishing Group UK 2021-06-23 /pmc/articles/PMC8222386/ /pubmed/34162879 http://dx.doi.org/10.1038/s41467-021-24212-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kaberniuk, Andrii A. Baloban, Mikhail Monakhov, Mikhail V. Shcherbakova, Daria M. Verkhusha, Vladislav V. Single-component near-infrared optogenetic systems for gene transcription regulation |
title | Single-component near-infrared optogenetic systems for gene transcription regulation |
title_full | Single-component near-infrared optogenetic systems for gene transcription regulation |
title_fullStr | Single-component near-infrared optogenetic systems for gene transcription regulation |
title_full_unstemmed | Single-component near-infrared optogenetic systems for gene transcription regulation |
title_short | Single-component near-infrared optogenetic systems for gene transcription regulation |
title_sort | single-component near-infrared optogenetic systems for gene transcription regulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222386/ https://www.ncbi.nlm.nih.gov/pubmed/34162879 http://dx.doi.org/10.1038/s41467-021-24212-7 |
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