Cargando…
Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production
Stimulator of interferon genes (STING) is a DNA sensor that responds to pathogens and induces type I interferon production. Herein, the role of STING in house dust mite extract (HDM)-induced allergic asthma was investigated. C57BL/6 wild-type (WT) and Sting(−/−) mice were intratracheally sensitized...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222396/ https://www.ncbi.nlm.nih.gov/pubmed/34162937 http://dx.doi.org/10.1038/s41598-021-92561-w |
_version_ | 1783711488279576576 |
---|---|
author | Nunokawa, Hiroki Murakami, Yusuke Ishii, Takashi Narita, Tomoya Ishii, Haruyuki Takizawa, Hajime Yamashita, Naomi |
author_facet | Nunokawa, Hiroki Murakami, Yusuke Ishii, Takashi Narita, Tomoya Ishii, Haruyuki Takizawa, Hajime Yamashita, Naomi |
author_sort | Nunokawa, Hiroki |
collection | PubMed |
description | Stimulator of interferon genes (STING) is a DNA sensor that responds to pathogens and induces type I interferon production. Herein, the role of STING in house dust mite extract (HDM)-induced allergic asthma was investigated. C57BL/6 wild-type (WT) and Sting(−/−) mice were intratracheally sensitized with HDM, and the bronchoalveolar lavage fluid (BALF), sera, lungs, and mediastinal lymph nodes (MLNs) were analyzed. The total and HDM-specific serum IgE levels were lower in Sting(−/−) mice than in WT mice. B cell and IgE-positive B cell proportion in BALF and MLNs, respectively, was significantly lower in Sting(−/−) mice than in WT mice. Additionally, cyclic GMP-AMP, a STING ligand, augmented total and HDM-specific serum IgE levels and B cell proportion in BALF when applied in combination with HDM. To elucidate the role of STING in IgE production, follicular helper T (Tfh) cells, which are involved in B cell maturation, were investigated. Tfh cell proportion in MLNs decreased in Sting(−/−) mice, and IL-4 and IL-13 production by HDM-restimulated MLN cells from HDM-sensitized mice was decreased in Sting(−/−) mice compared with WT mice. Thus, STING plays an important role in the maturation and class switching of IgE-producing B cells in allergic inflammation via Tfh cells. |
format | Online Article Text |
id | pubmed-8222396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-82223962021-07-02 Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production Nunokawa, Hiroki Murakami, Yusuke Ishii, Takashi Narita, Tomoya Ishii, Haruyuki Takizawa, Hajime Yamashita, Naomi Sci Rep Article Stimulator of interferon genes (STING) is a DNA sensor that responds to pathogens and induces type I interferon production. Herein, the role of STING in house dust mite extract (HDM)-induced allergic asthma was investigated. C57BL/6 wild-type (WT) and Sting(−/−) mice were intratracheally sensitized with HDM, and the bronchoalveolar lavage fluid (BALF), sera, lungs, and mediastinal lymph nodes (MLNs) were analyzed. The total and HDM-specific serum IgE levels were lower in Sting(−/−) mice than in WT mice. B cell and IgE-positive B cell proportion in BALF and MLNs, respectively, was significantly lower in Sting(−/−) mice than in WT mice. Additionally, cyclic GMP-AMP, a STING ligand, augmented total and HDM-specific serum IgE levels and B cell proportion in BALF when applied in combination with HDM. To elucidate the role of STING in IgE production, follicular helper T (Tfh) cells, which are involved in B cell maturation, were investigated. Tfh cell proportion in MLNs decreased in Sting(−/−) mice, and IL-4 and IL-13 production by HDM-restimulated MLN cells from HDM-sensitized mice was decreased in Sting(−/−) mice compared with WT mice. Thus, STING plays an important role in the maturation and class switching of IgE-producing B cells in allergic inflammation via Tfh cells. Nature Publishing Group UK 2021-06-23 /pmc/articles/PMC8222396/ /pubmed/34162937 http://dx.doi.org/10.1038/s41598-021-92561-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Nunokawa, Hiroki Murakami, Yusuke Ishii, Takashi Narita, Tomoya Ishii, Haruyuki Takizawa, Hajime Yamashita, Naomi Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production |
title | Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production |
title_full | Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production |
title_fullStr | Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production |
title_full_unstemmed | Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production |
title_short | Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production |
title_sort | crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced ige production |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222396/ https://www.ncbi.nlm.nih.gov/pubmed/34162937 http://dx.doi.org/10.1038/s41598-021-92561-w |
work_keys_str_mv | AT nunokawahiroki crucialroleofstimulatorofinterferongenesdependentsignalinginhousedustmiteextractinducedigeproduction AT murakamiyusuke crucialroleofstimulatorofinterferongenesdependentsignalinginhousedustmiteextractinducedigeproduction AT ishiitakashi crucialroleofstimulatorofinterferongenesdependentsignalinginhousedustmiteextractinducedigeproduction AT naritatomoya crucialroleofstimulatorofinterferongenesdependentsignalinginhousedustmiteextractinducedigeproduction AT ishiiharuyuki crucialroleofstimulatorofinterferongenesdependentsignalinginhousedustmiteextractinducedigeproduction AT takizawahajime crucialroleofstimulatorofinterferongenesdependentsignalinginhousedustmiteextractinducedigeproduction AT yamashitanaomi crucialroleofstimulatorofinterferongenesdependentsignalinginhousedustmiteextractinducedigeproduction |