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N(6)-Methyladenosine Associated Silencing of miR-193b Promotes Cervical Cancer Aggressiveness by Targeting CCND1

OBJECTIVE: Cervical cancer is a frequently encountered gynecological malignancy as a major contributor to cancer-related deaths in women. This study focuses on how miR-193b promotes cervical cancer aggressiveness as well as the role of m(6)A in miR-193b silencing. METHODS: Cervical cancer samples an...

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Autores principales: Huang, Chunxian, Liang, Jinxiao, Lin, Shaodan, Wang, Dongyan, Xie, Qingsheng, Lin, Zhongqiu, Yao, Tingting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222573/
https://www.ncbi.nlm.nih.gov/pubmed/34178650
http://dx.doi.org/10.3389/fonc.2021.666597
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author Huang, Chunxian
Liang, Jinxiao
Lin, Shaodan
Wang, Dongyan
Xie, Qingsheng
Lin, Zhongqiu
Yao, Tingting
author_facet Huang, Chunxian
Liang, Jinxiao
Lin, Shaodan
Wang, Dongyan
Xie, Qingsheng
Lin, Zhongqiu
Yao, Tingting
author_sort Huang, Chunxian
collection PubMed
description OBJECTIVE: Cervical cancer is a frequently encountered gynecological malignancy as a major contributor to cancer-related deaths in women. This study focuses on how miR-193b promotes cervical cancer aggressiveness as well as the role of m(6)A in miR-193b silencing. METHODS: Cervical cancer samples and the matching adjacent normal cervical tissues were used to determine the significance of miR-193b in cervical cancer. The CCK-8 assay, cell cycle analysis, qRT-PCR, Western blot assay, IHC, RIP, and xenograft models were utilized to explore the impact of miR-193b in cervical cancer and how m(6)A regulates miR-193b expression. Luciferase reporter assays, qRT-PCR, and Western blotting were enlisted to study the interaction between miR-193b and CCND1. RESULTS: Our study suggested that lower miR-193b expressions were strongly linked to more advanced cervical cancer stages and the presence of deeper stromal invasion. miR-193b functions as a tumor suppressor that is regulated by m(6)A methylation in cervical tumors. METTL3 modulates miR-193b mature process in an m(6)A-dependent manner. Reintroduction of miR-193b profoundly inhibits tumorigenesis of cervical cancer cells both in vivo and in vitro through CCND1 targeting. CONCLUSIONS: m(6)A associated downregulation of miR-193b promotes cervical cancer aggressiveness by targeting CCND1.
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spelling pubmed-82225732021-06-25 N(6)-Methyladenosine Associated Silencing of miR-193b Promotes Cervical Cancer Aggressiveness by Targeting CCND1 Huang, Chunxian Liang, Jinxiao Lin, Shaodan Wang, Dongyan Xie, Qingsheng Lin, Zhongqiu Yao, Tingting Front Oncol Oncology OBJECTIVE: Cervical cancer is a frequently encountered gynecological malignancy as a major contributor to cancer-related deaths in women. This study focuses on how miR-193b promotes cervical cancer aggressiveness as well as the role of m(6)A in miR-193b silencing. METHODS: Cervical cancer samples and the matching adjacent normal cervical tissues were used to determine the significance of miR-193b in cervical cancer. The CCK-8 assay, cell cycle analysis, qRT-PCR, Western blot assay, IHC, RIP, and xenograft models were utilized to explore the impact of miR-193b in cervical cancer and how m(6)A regulates miR-193b expression. Luciferase reporter assays, qRT-PCR, and Western blotting were enlisted to study the interaction between miR-193b and CCND1. RESULTS: Our study suggested that lower miR-193b expressions were strongly linked to more advanced cervical cancer stages and the presence of deeper stromal invasion. miR-193b functions as a tumor suppressor that is regulated by m(6)A methylation in cervical tumors. METTL3 modulates miR-193b mature process in an m(6)A-dependent manner. Reintroduction of miR-193b profoundly inhibits tumorigenesis of cervical cancer cells both in vivo and in vitro through CCND1 targeting. CONCLUSIONS: m(6)A associated downregulation of miR-193b promotes cervical cancer aggressiveness by targeting CCND1. Frontiers Media S.A. 2021-06-10 /pmc/articles/PMC8222573/ /pubmed/34178650 http://dx.doi.org/10.3389/fonc.2021.666597 Text en Copyright © 2021 Huang, Liang, Lin, Wang, Xie, Lin and Yao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Huang, Chunxian
Liang, Jinxiao
Lin, Shaodan
Wang, Dongyan
Xie, Qingsheng
Lin, Zhongqiu
Yao, Tingting
N(6)-Methyladenosine Associated Silencing of miR-193b Promotes Cervical Cancer Aggressiveness by Targeting CCND1
title N(6)-Methyladenosine Associated Silencing of miR-193b Promotes Cervical Cancer Aggressiveness by Targeting CCND1
title_full N(6)-Methyladenosine Associated Silencing of miR-193b Promotes Cervical Cancer Aggressiveness by Targeting CCND1
title_fullStr N(6)-Methyladenosine Associated Silencing of miR-193b Promotes Cervical Cancer Aggressiveness by Targeting CCND1
title_full_unstemmed N(6)-Methyladenosine Associated Silencing of miR-193b Promotes Cervical Cancer Aggressiveness by Targeting CCND1
title_short N(6)-Methyladenosine Associated Silencing of miR-193b Promotes Cervical Cancer Aggressiveness by Targeting CCND1
title_sort n(6)-methyladenosine associated silencing of mir-193b promotes cervical cancer aggressiveness by targeting ccnd1
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222573/
https://www.ncbi.nlm.nih.gov/pubmed/34178650
http://dx.doi.org/10.3389/fonc.2021.666597
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