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Application of CRISPR/Cas9 System for Plasmid Elimination and Bacterial Killing of Bacillus cereus Group Strains

The CRISPR-Cas system has been widely applied in prokaryotic genome editing with its high efficiency and easy operation. We constructed some “scissors plasmids” via using the temperature-sensitive pJOE8999 shuttle plasmid, which carry the different 20nt (N20) guiding the Cas9 nuclease as a scissors...

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Detalles Bibliográficos
Autores principales:	Wang, Xiaojing, Lyu, Yufei, Wang, Siya, Zheng, Qingfang, Feng, Erling, Zhu, Li, Pan, Chao, Wang, Shenghou, Wang, Dongshu, Liu, Xiankai, Wang, Hengliang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222586/ https://www.ncbi.nlm.nih.gov/pubmed/34177818 http://dx.doi.org/10.3389/fmicb.2021.536357

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