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Auxiliary Subunits Control Function and Subcellular Distribution of AMPA Receptor Complexes in NG2 Glia of the Developing Hippocampus

Synaptic and axonal glutamatergic signaling to NG2 glia in white matter is critical for the cells’ differentiation and activity dependent myelination. However, in gray matter the impact of neuron-to-NG2 glia signaling is still elusive, because most of these cells keep their non-myelinating phenotype...

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Autores principales: Hardt, Stefan, Tascio, Dario, Passlick, Stefan, Timmermann, Aline, Jabs, Ronald, Steinhäuser, Christian, Seifert, Gerald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222826/
https://www.ncbi.nlm.nih.gov/pubmed/34177466
http://dx.doi.org/10.3389/fncel.2021.669717
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author Hardt, Stefan
Tascio, Dario
Passlick, Stefan
Timmermann, Aline
Jabs, Ronald
Steinhäuser, Christian
Seifert, Gerald
author_facet Hardt, Stefan
Tascio, Dario
Passlick, Stefan
Timmermann, Aline
Jabs, Ronald
Steinhäuser, Christian
Seifert, Gerald
author_sort Hardt, Stefan
collection PubMed
description Synaptic and axonal glutamatergic signaling to NG2 glia in white matter is critical for the cells’ differentiation and activity dependent myelination. However, in gray matter the impact of neuron-to-NG2 glia signaling is still elusive, because most of these cells keep their non-myelinating phenotype throughout live. Early in postnatal development, hippocampal NG2 glia express AMPA receptors with a significant Ca(2+) permeability allowing for plasticity of the neuron-glia synapses, but whether this property changes by adulthood is not known. Moreover, it is unclear whether NG2 glia express auxiliary transmembrane AMPA receptor related proteins (TARPs), which modify AMPA receptor properties, including their Ca(2+) permeability. Through combined molecular and functional analyses, here we show that hippocampal NG2 glia abundantly express TARPs γ4, γ7, and γ8 as well as cornichon (CNIH)-2. TARP γ8 undergoes profound downregulation during development. Receptors of adult NG2 glia showed an increased sensitivity to blockers of Ca(2+) permeable AMPA receptors, but this increase mainly concerned receptors located close to the soma. Evoked synaptic currents of NG2 glia were also sensitive to blockers of Ca(2+) permeable AMPA receptors. The presence of AMPA receptors with varying Ca(2+) permeability during postnatal maturation may be important for the cells’ ability to sense and respond to local glutamatergic activity and for regulating process motility, differentiation, and proliferation.
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spelling pubmed-82228262021-06-25 Auxiliary Subunits Control Function and Subcellular Distribution of AMPA Receptor Complexes in NG2 Glia of the Developing Hippocampus Hardt, Stefan Tascio, Dario Passlick, Stefan Timmermann, Aline Jabs, Ronald Steinhäuser, Christian Seifert, Gerald Front Cell Neurosci Neuroscience Synaptic and axonal glutamatergic signaling to NG2 glia in white matter is critical for the cells’ differentiation and activity dependent myelination. However, in gray matter the impact of neuron-to-NG2 glia signaling is still elusive, because most of these cells keep their non-myelinating phenotype throughout live. Early in postnatal development, hippocampal NG2 glia express AMPA receptors with a significant Ca(2+) permeability allowing for plasticity of the neuron-glia synapses, but whether this property changes by adulthood is not known. Moreover, it is unclear whether NG2 glia express auxiliary transmembrane AMPA receptor related proteins (TARPs), which modify AMPA receptor properties, including their Ca(2+) permeability. Through combined molecular and functional analyses, here we show that hippocampal NG2 glia abundantly express TARPs γ4, γ7, and γ8 as well as cornichon (CNIH)-2. TARP γ8 undergoes profound downregulation during development. Receptors of adult NG2 glia showed an increased sensitivity to blockers of Ca(2+) permeable AMPA receptors, but this increase mainly concerned receptors located close to the soma. Evoked synaptic currents of NG2 glia were also sensitive to blockers of Ca(2+) permeable AMPA receptors. The presence of AMPA receptors with varying Ca(2+) permeability during postnatal maturation may be important for the cells’ ability to sense and respond to local glutamatergic activity and for regulating process motility, differentiation, and proliferation. Frontiers Media S.A. 2021-06-10 /pmc/articles/PMC8222826/ /pubmed/34177466 http://dx.doi.org/10.3389/fncel.2021.669717 Text en Copyright © 2021 Hardt, Tascio, Passlick, Timmermann, Jabs, Steinhäuser and Seifert. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Hardt, Stefan
Tascio, Dario
Passlick, Stefan
Timmermann, Aline
Jabs, Ronald
Steinhäuser, Christian
Seifert, Gerald
Auxiliary Subunits Control Function and Subcellular Distribution of AMPA Receptor Complexes in NG2 Glia of the Developing Hippocampus
title Auxiliary Subunits Control Function and Subcellular Distribution of AMPA Receptor Complexes in NG2 Glia of the Developing Hippocampus
title_full Auxiliary Subunits Control Function and Subcellular Distribution of AMPA Receptor Complexes in NG2 Glia of the Developing Hippocampus
title_fullStr Auxiliary Subunits Control Function and Subcellular Distribution of AMPA Receptor Complexes in NG2 Glia of the Developing Hippocampus
title_full_unstemmed Auxiliary Subunits Control Function and Subcellular Distribution of AMPA Receptor Complexes in NG2 Glia of the Developing Hippocampus
title_short Auxiliary Subunits Control Function and Subcellular Distribution of AMPA Receptor Complexes in NG2 Glia of the Developing Hippocampus
title_sort auxiliary subunits control function and subcellular distribution of ampa receptor complexes in ng2 glia of the developing hippocampus
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222826/
https://www.ncbi.nlm.nih.gov/pubmed/34177466
http://dx.doi.org/10.3389/fncel.2021.669717
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