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Genome-Wide Association Study on Root Traits Under Different Growing Environments in Wheat (Triticum aestivum L.)

Plant roots are critical for water and nutrient acquisition, environmental adaptation, and yield formation. Herein, 196 wheat accessions from the Huang-Huai Wheat Region of China were collected to investigate six root traits at seedling stage under three growing environments [indoor hydroponic cultu...

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Autores principales: Xu, Fengdan, Chen, Shulin, Yang, Xiwen, Zhou, Sumei, Wang, Junsen, Zhang, Ziliang, Huang, Yuan, Song, Miao, Zhang, Jun, Zhan, Kehui, He, Dexian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222912/
https://www.ncbi.nlm.nih.gov/pubmed/34178022
http://dx.doi.org/10.3389/fgene.2021.646712
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author Xu, Fengdan
Chen, Shulin
Yang, Xiwen
Zhou, Sumei
Wang, Junsen
Zhang, Ziliang
Huang, Yuan
Song, Miao
Zhang, Jun
Zhan, Kehui
He, Dexian
author_facet Xu, Fengdan
Chen, Shulin
Yang, Xiwen
Zhou, Sumei
Wang, Junsen
Zhang, Ziliang
Huang, Yuan
Song, Miao
Zhang, Jun
Zhan, Kehui
He, Dexian
author_sort Xu, Fengdan
collection PubMed
description Plant roots are critical for water and nutrient acquisition, environmental adaptation, and yield formation. Herein, 196 wheat accessions from the Huang-Huai Wheat Region of China were collected to investigate six root traits at seedling stage under three growing environments [indoor hydroponic culture (IHC), outdoor hydroponic culture (OHC), and outdoor pot culture (OPC)] and the root dry weight (RDW) under OPC at four growth stages and four yield traits in four environments. Additionally, a genome-wide association study was performed with a Wheat 660K SNP Array. The results showed that the root traits varied most under OPC, followed by those under both OHC and IHC, and root elongation under hydroponic culture was faster than that under pot culture. Root traits under OHC might help predict those under OPC. Moreover, root traits were significantly negatively correlated with grain yield (GY) and grains per spike (GPS), positively correlated with thousand-kernel weight (TKW), and weakly correlated with number of spikes per area (SPA). Twelve stable chromosomal regions associated with the root traits were detected on chromosomes 1D, 2A, 4A, 4B, 5B, 6D, and unmapped markers. Among them, a stable chromosomal interval from 737.85 to 742.00 Mb on chromosome 4A, which regulated total root length (TRL), was identified under three growing environments. Linkage disequilibrium (LD) blocks were used to identify 27 genes related to root development. Three genes TraesCS4A02G484200, TraesCS4A02G484800, TraesCS4A02G493800, and TraesCS4A02G493900, are involved in cell elongation and differentiation and expressed at high levels in root tissues. Another vital co-localization interval on chromosome 5B (397.72–410.88 Mb) was associated with not only RDW under OHC and OPC but also TKW.
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spelling pubmed-82229122021-06-25 Genome-Wide Association Study on Root Traits Under Different Growing Environments in Wheat (Triticum aestivum L.) Xu, Fengdan Chen, Shulin Yang, Xiwen Zhou, Sumei Wang, Junsen Zhang, Ziliang Huang, Yuan Song, Miao Zhang, Jun Zhan, Kehui He, Dexian Front Genet Genetics Plant roots are critical for water and nutrient acquisition, environmental adaptation, and yield formation. Herein, 196 wheat accessions from the Huang-Huai Wheat Region of China were collected to investigate six root traits at seedling stage under three growing environments [indoor hydroponic culture (IHC), outdoor hydroponic culture (OHC), and outdoor pot culture (OPC)] and the root dry weight (RDW) under OPC at four growth stages and four yield traits in four environments. Additionally, a genome-wide association study was performed with a Wheat 660K SNP Array. The results showed that the root traits varied most under OPC, followed by those under both OHC and IHC, and root elongation under hydroponic culture was faster than that under pot culture. Root traits under OHC might help predict those under OPC. Moreover, root traits were significantly negatively correlated with grain yield (GY) and grains per spike (GPS), positively correlated with thousand-kernel weight (TKW), and weakly correlated with number of spikes per area (SPA). Twelve stable chromosomal regions associated with the root traits were detected on chromosomes 1D, 2A, 4A, 4B, 5B, 6D, and unmapped markers. Among them, a stable chromosomal interval from 737.85 to 742.00 Mb on chromosome 4A, which regulated total root length (TRL), was identified under three growing environments. Linkage disequilibrium (LD) blocks were used to identify 27 genes related to root development. Three genes TraesCS4A02G484200, TraesCS4A02G484800, TraesCS4A02G493800, and TraesCS4A02G493900, are involved in cell elongation and differentiation and expressed at high levels in root tissues. Another vital co-localization interval on chromosome 5B (397.72–410.88 Mb) was associated with not only RDW under OHC and OPC but also TKW. Frontiers Media S.A. 2021-06-10 /pmc/articles/PMC8222912/ /pubmed/34178022 http://dx.doi.org/10.3389/fgene.2021.646712 Text en Copyright © 2021 Xu, Chen, Yang, Zhou, Wang, Zhang, Huang, Song, Zhang, Zhan and He. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Xu, Fengdan
Chen, Shulin
Yang, Xiwen
Zhou, Sumei
Wang, Junsen
Zhang, Ziliang
Huang, Yuan
Song, Miao
Zhang, Jun
Zhan, Kehui
He, Dexian
Genome-Wide Association Study on Root Traits Under Different Growing Environments in Wheat (Triticum aestivum L.)
title Genome-Wide Association Study on Root Traits Under Different Growing Environments in Wheat (Triticum aestivum L.)
title_full Genome-Wide Association Study on Root Traits Under Different Growing Environments in Wheat (Triticum aestivum L.)
title_fullStr Genome-Wide Association Study on Root Traits Under Different Growing Environments in Wheat (Triticum aestivum L.)
title_full_unstemmed Genome-Wide Association Study on Root Traits Under Different Growing Environments in Wheat (Triticum aestivum L.)
title_short Genome-Wide Association Study on Root Traits Under Different Growing Environments in Wheat (Triticum aestivum L.)
title_sort genome-wide association study on root traits under different growing environments in wheat (triticum aestivum l.)
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222912/
https://www.ncbi.nlm.nih.gov/pubmed/34178022
http://dx.doi.org/10.3389/fgene.2021.646712
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