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Identification and bioinformatic analysis of neprilysin and neprilysin-like metalloendopeptidases in Drosophila melanogaster
The neprilysin (M13) family of metalloendopeptidases comprises highly conserved ectoenzymes that cleave and thereby inactivate many physiologically relevant peptides in the extracellular space. Impaired neprilysin activity is associated with numerous human diseases. Here, we present a comprehensive...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223033/ https://www.ncbi.nlm.nih.gov/pubmed/34189422 http://dx.doi.org/10.17912/micropub.biology.000410 |
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author | Meyer, Heiko Buhr, Annika Callaerts, Patrick Schiemann, Ronja Wolfner, Mariana F. Marygold, Steven J. |
author_facet | Meyer, Heiko Buhr, Annika Callaerts, Patrick Schiemann, Ronja Wolfner, Mariana F. Marygold, Steven J. |
author_sort | Meyer, Heiko |
collection | PubMed |
description | The neprilysin (M13) family of metalloendopeptidases comprises highly conserved ectoenzymes that cleave and thereby inactivate many physiologically relevant peptides in the extracellular space. Impaired neprilysin activity is associated with numerous human diseases. Here, we present a comprehensive list and classification of M13 family members in Drosophila melanogaster. Seven Neprilysin (Nep) genes encode active peptidases, while 21 Neprilysin-like (Nepl) genes encode proteins predicted to be catalytically inactive. RNAseq data demonstrate that all 28 genes are expressed during development, often in a tissue-specific pattern. Most Nep proteins possess a transmembrane domain, whereas almost all Nepl proteins are predicted to be secreted. |
format | Online Article Text |
id | pubmed-8223033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-82230332021-06-28 Identification and bioinformatic analysis of neprilysin and neprilysin-like metalloendopeptidases in Drosophila melanogaster Meyer, Heiko Buhr, Annika Callaerts, Patrick Schiemann, Ronja Wolfner, Mariana F. Marygold, Steven J. MicroPubl Biol New Finding The neprilysin (M13) family of metalloendopeptidases comprises highly conserved ectoenzymes that cleave and thereby inactivate many physiologically relevant peptides in the extracellular space. Impaired neprilysin activity is associated with numerous human diseases. Here, we present a comprehensive list and classification of M13 family members in Drosophila melanogaster. Seven Neprilysin (Nep) genes encode active peptidases, while 21 Neprilysin-like (Nepl) genes encode proteins predicted to be catalytically inactive. RNAseq data demonstrate that all 28 genes are expressed during development, often in a tissue-specific pattern. Most Nep proteins possess a transmembrane domain, whereas almost all Nepl proteins are predicted to be secreted. Caltech Library 2021-06-23 /pmc/articles/PMC8223033/ /pubmed/34189422 http://dx.doi.org/10.17912/micropub.biology.000410 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Meyer, Heiko Buhr, Annika Callaerts, Patrick Schiemann, Ronja Wolfner, Mariana F. Marygold, Steven J. Identification and bioinformatic analysis of neprilysin and neprilysin-like metalloendopeptidases in Drosophila melanogaster |
title | Identification and bioinformatic analysis of neprilysin and neprilysin-like metalloendopeptidases in Drosophila melanogaster |
title_full | Identification and bioinformatic analysis of neprilysin and neprilysin-like metalloendopeptidases in Drosophila melanogaster |
title_fullStr | Identification and bioinformatic analysis of neprilysin and neprilysin-like metalloendopeptidases in Drosophila melanogaster |
title_full_unstemmed | Identification and bioinformatic analysis of neprilysin and neprilysin-like metalloendopeptidases in Drosophila melanogaster |
title_short | Identification and bioinformatic analysis of neprilysin and neprilysin-like metalloendopeptidases in Drosophila melanogaster |
title_sort | identification and bioinformatic analysis of neprilysin and neprilysin-like metalloendopeptidases in drosophila melanogaster |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223033/ https://www.ncbi.nlm.nih.gov/pubmed/34189422 http://dx.doi.org/10.17912/micropub.biology.000410 |
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