Cargando…

Glucose-Limited Fed-Batch Cultivation Strategy to Mimic Large-Scale Effects in Escherichia coli Linked to Accumulation of Non-Canonical Branched-Chain Amino Acids by Combination of Pyruvate Pulses and Dissolved Oxygen Limitation

Insufficient mixing in large-scale bioreactors provokes gradient zones of substrate, dissolved oxygen (DO), pH, and other parameters. E. coli responds to a high glucose, low oxygen feeding zone with the accumulation of mixed acid fermentation products, especially formate, but also with the synthesis...

Descripción completa

Detalles Bibliográficos
Autores principales:	García, Ángel Córcoles, Hauptmann, Peter, Neubauer, Peter
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223794/ https://www.ncbi.nlm.nih.gov/pubmed/34063744 http://dx.doi.org/10.3390/microorganisms9061110

Ejemplares similares

Molecular genetic approaches to decrease the uncontrolled misincorporation of non-canonical branched chain amino acids into recombinant mini-proinsulin expressed in Escherichia coli
por: García, Ángel Córcoles, et al.
Publicado: (2022)

High-cell-density fed-batch cultivations of Vibrio natriegens
por: Thiele, Isabel, et al.
Publicado: (2021)

Recombinant Peptide Production Softens Escherichia coli Cells and Increases Their Size during C-Limited Fed-Batch Cultivation
por: Weber, Andreas, et al.
Publicado: (2023)

Automated Conditional Screening of Multiple Escherichia coli Strains in Parallel Adaptive Fed-Batch Cultivations
por: Hans, Sebastian, et al.
Publicado: (2020)

Online automatic tuning and control for fed-batch cultivation
por: Soons, Zita I. T. A., et al.
Publicado: (2007)

Scale-up bioprocess development for production of the antibiotic valinomycin in Escherichia coli based on consistent fed-batch cultivations
por: Li, Jian, et al.
Publicado: (2015)

Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction
por: Resina, David, et al.
Publicado: (2007)

Production of recombinant human annexin V by fed-batch cultivation
por: Marder, Laura S, et al.
Publicado: (2014)

Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch
por: Ferreira Vaz, Michelle Rossana, et al.
Publicado: (2014)

Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures
por: Jahic, Mehmedalija, et al.
Publicado: (2003)

Implementation of a high cell density fed-batch for heterologous production of active [NiFe]-hydrogenase in Escherichia coli bioreactor cultivations
por: Fan, Qin, et al.
Publicado: (2022)

A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures
por: Krause, Mirja, et al.
Publicado: (2010)

Fed-Batch Cultivations of Rhodospirillum rubrum Under Multiple Nutrient-Limited Growth Conditions on Syngas as a Novel Option to Produce Poly(3-Hydroxybutyrate) (PHB)
por: Karmann, Stephanie, et al.
Publicado: (2019)

De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation
por: Duan, Yangkai, et al.
Publicado: (2011)

Fed-batch process for the psychrotolerant marine bacterium Pseudoalteromonas haloplanktis
por: Wilmes, Boris, et al.
Publicado: (2010)

Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures
por: Burgard, Jonas, et al.
Publicado: (2017)

Exploring the limits of conventional small-scale CHO fed-batch for accelerated on demand monoclonal antibody production
por: Mahé, Amélie, et al.
Publicado: (2021)

Sulfate limitation increases specific plasmid DNA yield and productivity in E. coli fed-batch processes
por: Gotsmy, Mathias, et al.
Publicado: (2023)

Substrate-Flexible Two-Stage Fed-Batch Cultivations for the Production of the PHA Copolymer P(HB-co-HHx) With Cupriavidus necator Re2058/pCB113
por: Santolin, Lara, et al.
Publicado: (2021)

Evaluation of gradual adaptation of mixed microalgae consortia cultivation using textile wastewater via fed batch operation
por: Kumar, Gopalakrishnan, et al.
Publicado: (2018)

The secretome of Pichia pastoris in fed‐batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time
por: Burgard, Jonas, et al.
Publicado: (2019)

Response coefficient analysis of a fed-batch bioreactor to dissolved oxygen perturbation in complementary cultures during PHB production
por: Patnaik, Pratap R
Publicado: (2008)

Cellulase activity mapping of Trichoderma reesei cultivated in sugar mixtures under fed-batch conditions
por: Jourdier, Etienne, et al.
Publicado: (2013)

Estimating Extrinsic Dyes for Fluorometric Online Monitoring of Antibody Aggregation in CHO Fed-Batch Cultivations
por: Schwab, Karen, et al.
Publicado: (2017)

Boosting Recombinant Inclusion Body Production—From Classical Fed-Batch Approach to Continuous Cultivation
por: Kopp, Julian, et al.
Publicado: (2019)

Effect of fed-batch and chemostat cultivation processes of C. glutamicum CP for L-leucine production
por: Zhang, Yufu, et al.
Publicado: (2021)

Real-time monitoring of the budding index in Saccharomyces cerevisiae batch cultivations with in situ microscopy
por: Marbà-Ardébol, Anna-Maria, et al.
Publicado: (2018)

High-throughput strategies for penicillin G acylase production in rE. coli fed-batch cultivations
por: Vélez, Ana Maria, et al.
Publicado: (2014)

Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures
por: Šiurkus, Juozas, et al.
Publicado: (2010)

Nutrient Limitation Mimics Artemisinin Tolerance in Malaria
por: Brown, Audrey C., et al.
Publicado: (2023)

Enhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch cultures
por: Pilarek, Maciej, et al.
Publicado: (2011)

Optimized polymer-based glucose release in microtiter plates for small-scale E. coli fed-batch cultivations
por: Keil, Timm, et al.
Publicado: (2020)

The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli
por: Krause, Mirja, et al.
Publicado: (2016)

Berberine Reduces Pyruvate-driven Hepatic Glucose Production by Limiting Mitochondrial Import of Pyruvate through Mitochondrial Pyruvate Carrier 1
por: Li, Aiyun, et al.
Publicado: (2018)

Production of soluble regulatory hydrogenase from Ralstonia eutropha in Escherichia coli using a fed-batch-based autoinduction system
por: Fan, Qin, et al.
Publicado: (2021)

Evaluation of three industrial Escherichia coli strains in fed-batch cultivations during high-level SOD protein production
por: Marisch, Karoline, et al.
Publicado: (2013)

Fed-batch like cultivation in a micro-bioreactor: screening conditions relevant for Escherichia coli based production processes
por: Toeroek, Csilla, et al.
Publicado: (2015)

A novel strategy and kinetics analysis of half‐fractional high cell density fed‐batch cultivation of Zygosaccharomyces rouxii
por: Li, Zhijiang, et al.
Publicado: (2018)

Optimization of EnBase Fed-Batch Cultivation to Improve Soluble Fraction Ratio of α-Luffin Ribosome Inactivating Protein
por: Barkhordari, Farzaneh, et al.
Publicado: (2018)

FeedER: a feedback-regulated enzyme-based slow-release system for fed-batch cultivation in microtiter plates
por: Jansen, Roman, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_lbljl77lm4ai0ujgso271pou4p