Effects of Dietary Inclusion Level of Microwave-Dried and Press-Defatted Black Soldier Fly (Hermetia illucens) Larvae Meal on Productive Performance, Cecal Volatile Fatty Acid Profile, and Egg Quality in Laying Hens

SIMPLE SUMMARY: The microwave drying method is convenient in terms of time efficiency, space, and low operational cost compared with the conventional air-drying method. For the same reasons, the microwave drying method has the potential for manufacturing insect meals that could be an alternative protein source in poultry. Therefore, we evaluated the productive performance, cecal volatile fatty acid (FA) profile, and egg quality in laying hens fed on microwave-dried Hermetia illucens larvae meal (HILM) at two different substitution levels (2% and 4%) of soybean meal. Similar productive performance with no negative effects on the nutritional and physical quality of eggs was observed from the study, indicating that microwave-dried HILM can be a potential ingredient in the diets of laying hens. However, further research is needed in improving the manufacturing process for better bioavailability of HILM and improved FA quality of eggs. ABSTRACT: Black soldier fly (Hermetia illucens) larvae meal (HILM) is a promising alternative to soybean meal (SBM). However, little information is available on the effect of microwave-dried HILM as a dietary protein source in the diets of laying hens. We studied the effect of dietary inclusion level of microwave-dried HILM on productive performance, cecal volatile fatty acid profile, egg quality, overall fatty acid profile, and heavy metal residues of the egg in laying hens. A total of 144 laying hens (25-week-old) were randomly assigned to three dietary groups (eight replicates and six birds/cage): a control diet, and two experimental diets in which SBM was replaced with 2% HILM (2HILM) and 4% HILM (4HILM). The laying hens that fed the HILM showed satisfactory results in productive performance and egg quality. Branched-chain fatty acid levels increased linearly (p < 0.001) with dietary treatment in the cecal digesta. Total monounsaturated fatty acid increased linearly (p < 0.01), while total polyunsaturated fatty acid decreased linearly (p < 0.01) in the eggs by dietary treatments. Heavy metals, magnesium, zinc, and aluminum were increased linearly with dietary treatment; however, undesirable heavy metals were under permissible levels. Thus, microwave-dried HILM could be a possible alternative to SBM in the diets of laying hens; however, improvements in fatty acid profile are needed.