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Pathogenicity Assessment of Colombian Strains of Candida auris in the Galleria mellonella Invertebrate Model

Candida auris, first described in 2009, is an opportunistic pathogenic yeast that causes nosocomial outbreaks around the world, with high mortality rates associated with therapeutic failure. In this study, we evaluated the pathogenicity of 107 isolates from two cities in Colombia, associated with fu...

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Autores principales: Carvajal, Silvia Katherine, Alvarado, Maira, Rodríguez, Yuli M., Parra-Giraldo, Claudia M., Varón, Carmen, Morales-López, Soraya E., Rodríguez, José Y., Gómez, Beatriz L., Escandón, Patricia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223996/
https://www.ncbi.nlm.nih.gov/pubmed/34063862
http://dx.doi.org/10.3390/jof7060401
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author Carvajal, Silvia Katherine
Alvarado, Maira
Rodríguez, Yuli M.
Parra-Giraldo, Claudia M.
Varón, Carmen
Morales-López, Soraya E.
Rodríguez, José Y.
Gómez, Beatriz L.
Escandón, Patricia
author_facet Carvajal, Silvia Katherine
Alvarado, Maira
Rodríguez, Yuli M.
Parra-Giraldo, Claudia M.
Varón, Carmen
Morales-López, Soraya E.
Rodríguez, José Y.
Gómez, Beatriz L.
Escandón, Patricia
author_sort Carvajal, Silvia Katherine
collection PubMed
description Candida auris, first described in 2009, is an opportunistic pathogenic yeast that causes nosocomial outbreaks around the world, with high mortality rates associated with therapeutic failure. In this study, we evaluated the pathogenicity of 107 isolates from two cities in Colombia, associated with fungemia or colonization processes; to achieve this, we used the Galleria mellonella invertebrate model to compare pathogenicity. Our results showed that less than half of the total isolates of C. auris presented a high pathogenicity compared to the reference strain SC5314, and most of those highly pathogenic strains were from colonization processes. We observed that there was formation of large aggregates of cells that cannot be disrupted easily, without statistically significant differences between the pathogenicity of the aggregated and non-aggregated strains. In addition, protease activity was observed in 100% of the C. auris strains; phospholipase and hemolysin activity were observed in 67.3 and 68.2% of the studied strains, respectively. In conclusion, these results highlight the utility of determining survival using G. mellonella, which allowed us to provide new information on the pathogenicity, enzymatic activity, and the relationship of the aggregated and non-aggregated phenotypes of C. auris in this model.
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spelling pubmed-82239962021-06-25 Pathogenicity Assessment of Colombian Strains of Candida auris in the Galleria mellonella Invertebrate Model Carvajal, Silvia Katherine Alvarado, Maira Rodríguez, Yuli M. Parra-Giraldo, Claudia M. Varón, Carmen Morales-López, Soraya E. Rodríguez, José Y. Gómez, Beatriz L. Escandón, Patricia J Fungi (Basel) Article Candida auris, first described in 2009, is an opportunistic pathogenic yeast that causes nosocomial outbreaks around the world, with high mortality rates associated with therapeutic failure. In this study, we evaluated the pathogenicity of 107 isolates from two cities in Colombia, associated with fungemia or colonization processes; to achieve this, we used the Galleria mellonella invertebrate model to compare pathogenicity. Our results showed that less than half of the total isolates of C. auris presented a high pathogenicity compared to the reference strain SC5314, and most of those highly pathogenic strains were from colonization processes. We observed that there was formation of large aggregates of cells that cannot be disrupted easily, without statistically significant differences between the pathogenicity of the aggregated and non-aggregated strains. In addition, protease activity was observed in 100% of the C. auris strains; phospholipase and hemolysin activity were observed in 67.3 and 68.2% of the studied strains, respectively. In conclusion, these results highlight the utility of determining survival using G. mellonella, which allowed us to provide new information on the pathogenicity, enzymatic activity, and the relationship of the aggregated and non-aggregated phenotypes of C. auris in this model. MDPI 2021-05-21 /pmc/articles/PMC8223996/ /pubmed/34063862 http://dx.doi.org/10.3390/jof7060401 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Carvajal, Silvia Katherine
Alvarado, Maira
Rodríguez, Yuli M.
Parra-Giraldo, Claudia M.
Varón, Carmen
Morales-López, Soraya E.
Rodríguez, José Y.
Gómez, Beatriz L.
Escandón, Patricia
Pathogenicity Assessment of Colombian Strains of Candida auris in the Galleria mellonella Invertebrate Model
title Pathogenicity Assessment of Colombian Strains of Candida auris in the Galleria mellonella Invertebrate Model
title_full Pathogenicity Assessment of Colombian Strains of Candida auris in the Galleria mellonella Invertebrate Model
title_fullStr Pathogenicity Assessment of Colombian Strains of Candida auris in the Galleria mellonella Invertebrate Model
title_full_unstemmed Pathogenicity Assessment of Colombian Strains of Candida auris in the Galleria mellonella Invertebrate Model
title_short Pathogenicity Assessment of Colombian Strains of Candida auris in the Galleria mellonella Invertebrate Model
title_sort pathogenicity assessment of colombian strains of candida auris in the galleria mellonella invertebrate model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223996/
https://www.ncbi.nlm.nih.gov/pubmed/34063862
http://dx.doi.org/10.3390/jof7060401
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