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12/111phiA Prophage Domestication Is Associated with Autoaggregation and Increased Ability to Produce Biofilm in Streptococcus agalactiae

CC17 Streptococcus agalactiae carrying group-A prophages is increasingly responsible for neonatal infections. To investigate the impact of the genetic features of a group-A prophage, we first conducted an in silico analysis of the genome of 12/111phiA, a group-A prophage carried by a strain responsi...

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Autores principales: Renard, Adélaïde, Diene, Seydina M., Courtier-Martinez, Luka, Gaillard, Julien Burlaud, Gbaguidi-Haore, Houssein, Mereghetti, Laurent, Quentin, Roland, Francois, Patrice, Van Der Mee-Marquet, Nathalie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223999/
https://www.ncbi.nlm.nih.gov/pubmed/34063935
http://dx.doi.org/10.3390/microorganisms9061112
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author Renard, Adélaïde
Diene, Seydina M.
Courtier-Martinez, Luka
Gaillard, Julien Burlaud
Gbaguidi-Haore, Houssein
Mereghetti, Laurent
Quentin, Roland
Francois, Patrice
Van Der Mee-Marquet, Nathalie
author_facet Renard, Adélaïde
Diene, Seydina M.
Courtier-Martinez, Luka
Gaillard, Julien Burlaud
Gbaguidi-Haore, Houssein
Mereghetti, Laurent
Quentin, Roland
Francois, Patrice
Van Der Mee-Marquet, Nathalie
author_sort Renard, Adélaïde
collection PubMed
description CC17 Streptococcus agalactiae carrying group-A prophages is increasingly responsible for neonatal infections. To investigate the impact of the genetic features of a group-A prophage, we first conducted an in silico analysis of the genome of 12/111phiA, a group-A prophage carried by a strain responsible for a bloodstream infection in a parturient. This revealed a Restriction Modification system, suggesting a prophage maintenance strategy and five ORFs of interest for the host and encoding a type II toxin antitoxin system RelB/YafQ, an endonuclease, an S-adenosylmethionine synthetase MetK, and an StrP-like adhesin. Using the WT strain cured from 12/111phiA and constructing deleted mutants for the ORFs of interest, and their complemented mutants, we demonstrated an impact of prophage features on growth characteristics, cell morphology and biofilm formation. Our findings argue in favor of 12/111phiA domestication by the host and a role of prophage features in cell autoaggregation, glycocalyx and biofilm formation. We suggest that lysogeny may promote GBS adaptation to the acid environment of the vagina, consequently colonizing and infecting neonates.
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spelling pubmed-82239992021-06-25 12/111phiA Prophage Domestication Is Associated with Autoaggregation and Increased Ability to Produce Biofilm in Streptococcus agalactiae Renard, Adélaïde Diene, Seydina M. Courtier-Martinez, Luka Gaillard, Julien Burlaud Gbaguidi-Haore, Houssein Mereghetti, Laurent Quentin, Roland Francois, Patrice Van Der Mee-Marquet, Nathalie Microorganisms Article CC17 Streptococcus agalactiae carrying group-A prophages is increasingly responsible for neonatal infections. To investigate the impact of the genetic features of a group-A prophage, we first conducted an in silico analysis of the genome of 12/111phiA, a group-A prophage carried by a strain responsible for a bloodstream infection in a parturient. This revealed a Restriction Modification system, suggesting a prophage maintenance strategy and five ORFs of interest for the host and encoding a type II toxin antitoxin system RelB/YafQ, an endonuclease, an S-adenosylmethionine synthetase MetK, and an StrP-like adhesin. Using the WT strain cured from 12/111phiA and constructing deleted mutants for the ORFs of interest, and their complemented mutants, we demonstrated an impact of prophage features on growth characteristics, cell morphology and biofilm formation. Our findings argue in favor of 12/111phiA domestication by the host and a role of prophage features in cell autoaggregation, glycocalyx and biofilm formation. We suggest that lysogeny may promote GBS adaptation to the acid environment of the vagina, consequently colonizing and infecting neonates. MDPI 2021-05-21 /pmc/articles/PMC8223999/ /pubmed/34063935 http://dx.doi.org/10.3390/microorganisms9061112 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Renard, Adélaïde
Diene, Seydina M.
Courtier-Martinez, Luka
Gaillard, Julien Burlaud
Gbaguidi-Haore, Houssein
Mereghetti, Laurent
Quentin, Roland
Francois, Patrice
Van Der Mee-Marquet, Nathalie
12/111phiA Prophage Domestication Is Associated with Autoaggregation and Increased Ability to Produce Biofilm in Streptococcus agalactiae
title 12/111phiA Prophage Domestication Is Associated with Autoaggregation and Increased Ability to Produce Biofilm in Streptococcus agalactiae
title_full 12/111phiA Prophage Domestication Is Associated with Autoaggregation and Increased Ability to Produce Biofilm in Streptococcus agalactiae
title_fullStr 12/111phiA Prophage Domestication Is Associated with Autoaggregation and Increased Ability to Produce Biofilm in Streptococcus agalactiae
title_full_unstemmed 12/111phiA Prophage Domestication Is Associated with Autoaggregation and Increased Ability to Produce Biofilm in Streptococcus agalactiae
title_short 12/111phiA Prophage Domestication Is Associated with Autoaggregation and Increased Ability to Produce Biofilm in Streptococcus agalactiae
title_sort 12/111phia prophage domestication is associated with autoaggregation and increased ability to produce biofilm in streptococcus agalactiae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223999/
https://www.ncbi.nlm.nih.gov/pubmed/34063935
http://dx.doi.org/10.3390/microorganisms9061112
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