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Oxidative Stress and Its Consequences in the Blood of Rats Irradiated with UV: Protective Effect of Cannabidiol

UVA/UVB radiation disturbs the redox balance of skin cells, and metabolic consequences can be transferred into the blood and internal tissues, especially after chronic skin exposure to UV radiation. Therefore, the aim of this study was to evaluate the effect of cannabidiol (CBD), an antioxidant and...

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Autores principales: Biernacki, Michał, Brzóska, Małgorzata Michalina, Markowska, Agnieszka, Gałażyn-Sidorczuk, Małgorzata, Cylwik, Bogdan, Gęgotek, Agnieszka, Skrzydlewska, Elżbieta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224002/
https://www.ncbi.nlm.nih.gov/pubmed/34063802
http://dx.doi.org/10.3390/antiox10060821
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author Biernacki, Michał
Brzóska, Małgorzata Michalina
Markowska, Agnieszka
Gałażyn-Sidorczuk, Małgorzata
Cylwik, Bogdan
Gęgotek, Agnieszka
Skrzydlewska, Elżbieta
author_facet Biernacki, Michał
Brzóska, Małgorzata Michalina
Markowska, Agnieszka
Gałażyn-Sidorczuk, Małgorzata
Cylwik, Bogdan
Gęgotek, Agnieszka
Skrzydlewska, Elżbieta
author_sort Biernacki, Michał
collection PubMed
description UVA/UVB radiation disturbs the redox balance of skin cells, and metabolic consequences can be transferred into the blood and internal tissues, especially after chronic skin exposure to UV radiation. Therefore, the aim of this study was to evaluate the effect of cannabidiol (CBD), an antioxidant and anti-inflammatory phytocannabinoid, on oxidative stress and its consequences in the blood of nude rats whose skin was exposed to UVA/UVB radiation for 4 weeks. It was shown that CBD penetrated the blood and in UVB-irradiated rats was preferentially located in the membranes of polymorphonuclear leukocytes, which promoted reduction of ROS generation and up-regulation of antioxidant ability by increasing the activity of glutathione reductase and thioredoxin reductase, while the level of reduced glutathione decreased by UV radiation. Consequently, reduction in UV-induced lipid peroxidation, assessed as 4-hydroxynonenal (4-HNE) and 8-isoprostane (8-isoPGF2α) as well as protein modifications, estimated as 4-HNE-protein adducts and protein carbonyl groups, was observed. CBD, by countering the UV-induced down-regulation of 2-arachidonylglycerol, promoted its antioxidant/anti-inflammatory effects by reducing CB1 and increasing PPARγ receptor activation and consequently ROS and TNF-α down-regulation. The results suggest that CBD applied topically to the skin minimizes redox changes not only at the skin level, but also at the systemic level.
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spelling pubmed-82240022021-06-25 Oxidative Stress and Its Consequences in the Blood of Rats Irradiated with UV: Protective Effect of Cannabidiol Biernacki, Michał Brzóska, Małgorzata Michalina Markowska, Agnieszka Gałażyn-Sidorczuk, Małgorzata Cylwik, Bogdan Gęgotek, Agnieszka Skrzydlewska, Elżbieta Antioxidants (Basel) Article UVA/UVB radiation disturbs the redox balance of skin cells, and metabolic consequences can be transferred into the blood and internal tissues, especially after chronic skin exposure to UV radiation. Therefore, the aim of this study was to evaluate the effect of cannabidiol (CBD), an antioxidant and anti-inflammatory phytocannabinoid, on oxidative stress and its consequences in the blood of nude rats whose skin was exposed to UVA/UVB radiation for 4 weeks. It was shown that CBD penetrated the blood and in UVB-irradiated rats was preferentially located in the membranes of polymorphonuclear leukocytes, which promoted reduction of ROS generation and up-regulation of antioxidant ability by increasing the activity of glutathione reductase and thioredoxin reductase, while the level of reduced glutathione decreased by UV radiation. Consequently, reduction in UV-induced lipid peroxidation, assessed as 4-hydroxynonenal (4-HNE) and 8-isoprostane (8-isoPGF2α) as well as protein modifications, estimated as 4-HNE-protein adducts and protein carbonyl groups, was observed. CBD, by countering the UV-induced down-regulation of 2-arachidonylglycerol, promoted its antioxidant/anti-inflammatory effects by reducing CB1 and increasing PPARγ receptor activation and consequently ROS and TNF-α down-regulation. The results suggest that CBD applied topically to the skin minimizes redox changes not only at the skin level, but also at the systemic level. MDPI 2021-05-21 /pmc/articles/PMC8224002/ /pubmed/34063802 http://dx.doi.org/10.3390/antiox10060821 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Biernacki, Michał
Brzóska, Małgorzata Michalina
Markowska, Agnieszka
Gałażyn-Sidorczuk, Małgorzata
Cylwik, Bogdan
Gęgotek, Agnieszka
Skrzydlewska, Elżbieta
Oxidative Stress and Its Consequences in the Blood of Rats Irradiated with UV: Protective Effect of Cannabidiol
title Oxidative Stress and Its Consequences in the Blood of Rats Irradiated with UV: Protective Effect of Cannabidiol
title_full Oxidative Stress and Its Consequences in the Blood of Rats Irradiated with UV: Protective Effect of Cannabidiol
title_fullStr Oxidative Stress and Its Consequences in the Blood of Rats Irradiated with UV: Protective Effect of Cannabidiol
title_full_unstemmed Oxidative Stress and Its Consequences in the Blood of Rats Irradiated with UV: Protective Effect of Cannabidiol
title_short Oxidative Stress and Its Consequences in the Blood of Rats Irradiated with UV: Protective Effect of Cannabidiol
title_sort oxidative stress and its consequences in the blood of rats irradiated with uv: protective effect of cannabidiol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224002/
https://www.ncbi.nlm.nih.gov/pubmed/34063802
http://dx.doi.org/10.3390/antiox10060821
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