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Regulatory Action of Plasma from Patients with Obesity and Diabetes towards Muscle Cells Differentiation and Bioenergetics Revealed by the C2C12 Cell Model and MicroRNA Analysis

Obesity and type 2 diabetes mellitus (T2DM) are often combined and pathologically affect many tissues due to changes in circulating bioactive molecules. In this work, we evaluated the effect of blood plasma from obese (OB) patients or from obese patients comorbid with diabetes (OBD) on skeletal musc...

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Autores principales: Khromova, Natalya V., Fedorov, Anton V., Ma, Yi, Kondratov, Kirill A., Prikhodko, Stanislava S., Ignatieva, Elena V., Artemyeva, Marina S., Anopova, Anna D., Neimark, Aleksandr E., Kostareva, Anna A., Babenko, Alina Yu., Dmitrieva, Renata I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224077/
https://www.ncbi.nlm.nih.gov/pubmed/34063883
http://dx.doi.org/10.3390/biom11060769
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author Khromova, Natalya V.
Fedorov, Anton V.
Ma, Yi
Kondratov, Kirill A.
Prikhodko, Stanislava S.
Ignatieva, Elena V.
Artemyeva, Marina S.
Anopova, Anna D.
Neimark, Aleksandr E.
Kostareva, Anna A.
Babenko, Alina Yu.
Dmitrieva, Renata I.
author_facet Khromova, Natalya V.
Fedorov, Anton V.
Ma, Yi
Kondratov, Kirill A.
Prikhodko, Stanislava S.
Ignatieva, Elena V.
Artemyeva, Marina S.
Anopova, Anna D.
Neimark, Aleksandr E.
Kostareva, Anna A.
Babenko, Alina Yu.
Dmitrieva, Renata I.
author_sort Khromova, Natalya V.
collection PubMed
description Obesity and type 2 diabetes mellitus (T2DM) are often combined and pathologically affect many tissues due to changes in circulating bioactive molecules. In this work, we evaluated the effect of blood plasma from obese (OB) patients or from obese patients comorbid with diabetes (OBD) on skeletal muscle function and metabolic state. We employed the mouse myoblasts C2C12 differentiation model to test the regulatory effect of plasma exposure at several levels: (1) cell morphology; (2) functional activity of mitochondria; (3) expression levels of several mitochondria regulators, i.e., Atgl, Pgc1b, and miR-378a-3p. Existing databases were used to computationally predict and analyze mir-378a-3p potential targets. We show that short-term exposure to OB or OBD patients’ plasma is sufficient to affect C2C12 properties. In fact, the expression of genes that regulate skeletal muscle differentiation and growth was downregulated in both OB- and OBD-treated cells, maximal mitochondrial respiration rate was downregulated in the OBD group, while in the OB group, a metabolic switch to glycolysis was detected. These alterations correlated with a decrease in ATGL and Pgc1b expression in the OB group and with an increase of miR-378a-3p levels in the OBD group.
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spelling pubmed-82240772021-06-25 Regulatory Action of Plasma from Patients with Obesity and Diabetes towards Muscle Cells Differentiation and Bioenergetics Revealed by the C2C12 Cell Model and MicroRNA Analysis Khromova, Natalya V. Fedorov, Anton V. Ma, Yi Kondratov, Kirill A. Prikhodko, Stanislava S. Ignatieva, Elena V. Artemyeva, Marina S. Anopova, Anna D. Neimark, Aleksandr E. Kostareva, Anna A. Babenko, Alina Yu. Dmitrieva, Renata I. Biomolecules Article Obesity and type 2 diabetes mellitus (T2DM) are often combined and pathologically affect many tissues due to changes in circulating bioactive molecules. In this work, we evaluated the effect of blood plasma from obese (OB) patients or from obese patients comorbid with diabetes (OBD) on skeletal muscle function and metabolic state. We employed the mouse myoblasts C2C12 differentiation model to test the regulatory effect of plasma exposure at several levels: (1) cell morphology; (2) functional activity of mitochondria; (3) expression levels of several mitochondria regulators, i.e., Atgl, Pgc1b, and miR-378a-3p. Existing databases were used to computationally predict and analyze mir-378a-3p potential targets. We show that short-term exposure to OB or OBD patients’ plasma is sufficient to affect C2C12 properties. In fact, the expression of genes that regulate skeletal muscle differentiation and growth was downregulated in both OB- and OBD-treated cells, maximal mitochondrial respiration rate was downregulated in the OBD group, while in the OB group, a metabolic switch to glycolysis was detected. These alterations correlated with a decrease in ATGL and Pgc1b expression in the OB group and with an increase of miR-378a-3p levels in the OBD group. MDPI 2021-05-21 /pmc/articles/PMC8224077/ /pubmed/34063883 http://dx.doi.org/10.3390/biom11060769 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khromova, Natalya V.
Fedorov, Anton V.
Ma, Yi
Kondratov, Kirill A.
Prikhodko, Stanislava S.
Ignatieva, Elena V.
Artemyeva, Marina S.
Anopova, Anna D.
Neimark, Aleksandr E.
Kostareva, Anna A.
Babenko, Alina Yu.
Dmitrieva, Renata I.
Regulatory Action of Plasma from Patients with Obesity and Diabetes towards Muscle Cells Differentiation and Bioenergetics Revealed by the C2C12 Cell Model and MicroRNA Analysis
title Regulatory Action of Plasma from Patients with Obesity and Diabetes towards Muscle Cells Differentiation and Bioenergetics Revealed by the C2C12 Cell Model and MicroRNA Analysis
title_full Regulatory Action of Plasma from Patients with Obesity and Diabetes towards Muscle Cells Differentiation and Bioenergetics Revealed by the C2C12 Cell Model and MicroRNA Analysis
title_fullStr Regulatory Action of Plasma from Patients with Obesity and Diabetes towards Muscle Cells Differentiation and Bioenergetics Revealed by the C2C12 Cell Model and MicroRNA Analysis
title_full_unstemmed Regulatory Action of Plasma from Patients with Obesity and Diabetes towards Muscle Cells Differentiation and Bioenergetics Revealed by the C2C12 Cell Model and MicroRNA Analysis
title_short Regulatory Action of Plasma from Patients with Obesity and Diabetes towards Muscle Cells Differentiation and Bioenergetics Revealed by the C2C12 Cell Model and MicroRNA Analysis
title_sort regulatory action of plasma from patients with obesity and diabetes towards muscle cells differentiation and bioenergetics revealed by the c2c12 cell model and microrna analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224077/
https://www.ncbi.nlm.nih.gov/pubmed/34063883
http://dx.doi.org/10.3390/biom11060769
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