Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9

CRISPR-Cas systems recognize foreign genetic material using CRISPR RNAs (crRNAs). In type II systems, a trans-activating crRNA (tracrRNA) hybridizes to crRNAs to drive their processing and utilization by Cas9. While analyzing Cas9-RNA complexes from Campylobacter jejuni, we discovered tracrRNA hybri...

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Detalles Bibliográficos
Autores principales: Jiao, Chunlei, Sharma, Sahil, Dugar, Gaurav, Peeck, Natalia L., Bischler, Thorsten, Wimmer, Franziska, Yu, Yanying, Barquist, Lars, Schoen, Christoph, Kurzai, Oliver, Sharma, Cynthia M., Beisel, Chase L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224270/
https://www.ncbi.nlm.nih.gov/pubmed/33906967
http://dx.doi.org/10.1126/science.abe7106

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