Cargando…
Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model
Additive manufacturing is widely used to produce highly complex structures. Moreover, this technology has proven its superiority in producing tools which can be used in different applications. We designed and produced an extrusion nozzle that allowed us to hot melt extrude drug-loaded tubes. The tub...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224298/ https://www.ncbi.nlm.nih.gov/pubmed/34064276 http://dx.doi.org/10.3390/pharmaceutics13060772 |
_version_ | 1783711858147983360 |
---|---|
author | Shaqour, Bahaa Aizawa, Juliana Guarch-Pérez, Clara Górecka, Żaneta Christophersen, Lars Martinet, Wim Choińska, Emilia Riool, Martijn Verleije, Bart Beyers, Koen Moser, Claus Święszkowski, Wojciech Zaat, Sebastian A. J. Cos, Paul |
author_facet | Shaqour, Bahaa Aizawa, Juliana Guarch-Pérez, Clara Górecka, Żaneta Christophersen, Lars Martinet, Wim Choińska, Emilia Riool, Martijn Verleije, Bart Beyers, Koen Moser, Claus Święszkowski, Wojciech Zaat, Sebastian A. J. Cos, Paul |
author_sort | Shaqour, Bahaa |
collection | PubMed |
description | Additive manufacturing is widely used to produce highly complex structures. Moreover, this technology has proven its superiority in producing tools which can be used in different applications. We designed and produced an extrusion nozzle that allowed us to hot melt extrude drug-loaded tubes. The tubes were an essential part of a new mouse ventilator-associated pneumonia (VAP) model. Ciprofloxacin (CPX) was selected for its expected activity against the pathogen Staphylococcus aureus and ease of incorporation into thermoplastic polyurethane (TPU). TPU was selected as the carrier polymer for its biocompatibility and use in a variety of medical devices such as tubing and catheters. The effect of loading CPX within the TPU polymeric matrix and the physicochemical properties of the produced tubes were investigated. CPX showed good thermal stability and in vitro activity in preventing S. aureus biofilm formation after loading within the tube’s polymeric matrix. Moreover, the produced tubes showed anti-infective efficacy in vivo. The produced tubes, which were extruded via our novel nozzle, were vital for the validation of our mouse VAP model. This model can be adopted to investigate other antibacterial and antibiofilm compounds incorporated in polymeric tubes using hot melt extrusion. |
format | Online Article Text |
id | pubmed-8224298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82242982021-06-25 Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model Shaqour, Bahaa Aizawa, Juliana Guarch-Pérez, Clara Górecka, Żaneta Christophersen, Lars Martinet, Wim Choińska, Emilia Riool, Martijn Verleije, Bart Beyers, Koen Moser, Claus Święszkowski, Wojciech Zaat, Sebastian A. J. Cos, Paul Pharmaceutics Article Additive manufacturing is widely used to produce highly complex structures. Moreover, this technology has proven its superiority in producing tools which can be used in different applications. We designed and produced an extrusion nozzle that allowed us to hot melt extrude drug-loaded tubes. The tubes were an essential part of a new mouse ventilator-associated pneumonia (VAP) model. Ciprofloxacin (CPX) was selected for its expected activity against the pathogen Staphylococcus aureus and ease of incorporation into thermoplastic polyurethane (TPU). TPU was selected as the carrier polymer for its biocompatibility and use in a variety of medical devices such as tubing and catheters. The effect of loading CPX within the TPU polymeric matrix and the physicochemical properties of the produced tubes were investigated. CPX showed good thermal stability and in vitro activity in preventing S. aureus biofilm formation after loading within the tube’s polymeric matrix. Moreover, the produced tubes showed anti-infective efficacy in vivo. The produced tubes, which were extruded via our novel nozzle, were vital for the validation of our mouse VAP model. This model can be adopted to investigate other antibacterial and antibiofilm compounds incorporated in polymeric tubes using hot melt extrusion. MDPI 2021-05-21 /pmc/articles/PMC8224298/ /pubmed/34064276 http://dx.doi.org/10.3390/pharmaceutics13060772 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shaqour, Bahaa Aizawa, Juliana Guarch-Pérez, Clara Górecka, Żaneta Christophersen, Lars Martinet, Wim Choińska, Emilia Riool, Martijn Verleije, Bart Beyers, Koen Moser, Claus Święszkowski, Wojciech Zaat, Sebastian A. J. Cos, Paul Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model |
title | Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model |
title_full | Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model |
title_fullStr | Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model |
title_full_unstemmed | Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model |
title_short | Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model |
title_sort | coupling additive manufacturing with hot melt extrusion technologies to validate a ventilator-associated pneumonia mouse model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224298/ https://www.ncbi.nlm.nih.gov/pubmed/34064276 http://dx.doi.org/10.3390/pharmaceutics13060772 |
work_keys_str_mv | AT shaqourbahaa couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT aizawajuliana couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT guarchperezclara couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT goreckazaneta couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT christophersenlars couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT martinetwim couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT choinskaemilia couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT rioolmartijn couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT verleijebart couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT beyerskoen couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT moserclaus couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT swieszkowskiwojciech couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT zaatsebastianaj couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel AT cospaul couplingadditivemanufacturingwithhotmeltextrusiontechnologiestovalidateaventilatorassociatedpneumoniamousemodel |