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Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model

Additive manufacturing is widely used to produce highly complex structures. Moreover, this technology has proven its superiority in producing tools which can be used in different applications. We designed and produced an extrusion nozzle that allowed us to hot melt extrude drug-loaded tubes. The tub...

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Autores principales: Shaqour, Bahaa, Aizawa, Juliana, Guarch-Pérez, Clara, Górecka, Żaneta, Christophersen, Lars, Martinet, Wim, Choińska, Emilia, Riool, Martijn, Verleije, Bart, Beyers, Koen, Moser, Claus, Święszkowski, Wojciech, Zaat, Sebastian A. J., Cos, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224298/
https://www.ncbi.nlm.nih.gov/pubmed/34064276
http://dx.doi.org/10.3390/pharmaceutics13060772
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author Shaqour, Bahaa
Aizawa, Juliana
Guarch-Pérez, Clara
Górecka, Żaneta
Christophersen, Lars
Martinet, Wim
Choińska, Emilia
Riool, Martijn
Verleije, Bart
Beyers, Koen
Moser, Claus
Święszkowski, Wojciech
Zaat, Sebastian A. J.
Cos, Paul
author_facet Shaqour, Bahaa
Aizawa, Juliana
Guarch-Pérez, Clara
Górecka, Żaneta
Christophersen, Lars
Martinet, Wim
Choińska, Emilia
Riool, Martijn
Verleije, Bart
Beyers, Koen
Moser, Claus
Święszkowski, Wojciech
Zaat, Sebastian A. J.
Cos, Paul
author_sort Shaqour, Bahaa
collection PubMed
description Additive manufacturing is widely used to produce highly complex structures. Moreover, this technology has proven its superiority in producing tools which can be used in different applications. We designed and produced an extrusion nozzle that allowed us to hot melt extrude drug-loaded tubes. The tubes were an essential part of a new mouse ventilator-associated pneumonia (VAP) model. Ciprofloxacin (CPX) was selected for its expected activity against the pathogen Staphylococcus aureus and ease of incorporation into thermoplastic polyurethane (TPU). TPU was selected as the carrier polymer for its biocompatibility and use in a variety of medical devices such as tubing and catheters. The effect of loading CPX within the TPU polymeric matrix and the physicochemical properties of the produced tubes were investigated. CPX showed good thermal stability and in vitro activity in preventing S. aureus biofilm formation after loading within the tube’s polymeric matrix. Moreover, the produced tubes showed anti-infective efficacy in vivo. The produced tubes, which were extruded via our novel nozzle, were vital for the validation of our mouse VAP model. This model can be adopted to investigate other antibacterial and antibiofilm compounds incorporated in polymeric tubes using hot melt extrusion.
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spelling pubmed-82242982021-06-25 Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model Shaqour, Bahaa Aizawa, Juliana Guarch-Pérez, Clara Górecka, Żaneta Christophersen, Lars Martinet, Wim Choińska, Emilia Riool, Martijn Verleije, Bart Beyers, Koen Moser, Claus Święszkowski, Wojciech Zaat, Sebastian A. J. Cos, Paul Pharmaceutics Article Additive manufacturing is widely used to produce highly complex structures. Moreover, this technology has proven its superiority in producing tools which can be used in different applications. We designed and produced an extrusion nozzle that allowed us to hot melt extrude drug-loaded tubes. The tubes were an essential part of a new mouse ventilator-associated pneumonia (VAP) model. Ciprofloxacin (CPX) was selected for its expected activity against the pathogen Staphylococcus aureus and ease of incorporation into thermoplastic polyurethane (TPU). TPU was selected as the carrier polymer for its biocompatibility and use in a variety of medical devices such as tubing and catheters. The effect of loading CPX within the TPU polymeric matrix and the physicochemical properties of the produced tubes were investigated. CPX showed good thermal stability and in vitro activity in preventing S. aureus biofilm formation after loading within the tube’s polymeric matrix. Moreover, the produced tubes showed anti-infective efficacy in vivo. The produced tubes, which were extruded via our novel nozzle, were vital for the validation of our mouse VAP model. This model can be adopted to investigate other antibacterial and antibiofilm compounds incorporated in polymeric tubes using hot melt extrusion. MDPI 2021-05-21 /pmc/articles/PMC8224298/ /pubmed/34064276 http://dx.doi.org/10.3390/pharmaceutics13060772 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shaqour, Bahaa
Aizawa, Juliana
Guarch-Pérez, Clara
Górecka, Żaneta
Christophersen, Lars
Martinet, Wim
Choińska, Emilia
Riool, Martijn
Verleije, Bart
Beyers, Koen
Moser, Claus
Święszkowski, Wojciech
Zaat, Sebastian A. J.
Cos, Paul
Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model
title Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model
title_full Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model
title_fullStr Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model
title_full_unstemmed Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model
title_short Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model
title_sort coupling additive manufacturing with hot melt extrusion technologies to validate a ventilator-associated pneumonia mouse model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224298/
https://www.ncbi.nlm.nih.gov/pubmed/34064276
http://dx.doi.org/10.3390/pharmaceutics13060772
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