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Expression of Bordetella pertussis Antigens Fused to Different Vectors and Their Effectiveness as Vaccines

Pertussis is an acute respiratory tract infection caused by Bordetella pertussis. Even though its current vaccine coverage is relatively broad, they still have some shortcomings such as short protection time and might be incapable of blocking the spread of the disease. In this study, we developed ne...

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Autores principales: Xu, Han, Huang, Jing, Liu, Zhaolu, Li, Xin, Wang, Kangfeng, Feng, Erling, Wu, Jun, Zhu, Li, Yao, Kaihu, Pan, Chao, Wang, Hengliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224380/
https://www.ncbi.nlm.nih.gov/pubmed/34064136
http://dx.doi.org/10.3390/vaccines9060542
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author Xu, Han
Huang, Jing
Liu, Zhaolu
Li, Xin
Wang, Kangfeng
Feng, Erling
Wu, Jun
Zhu, Li
Yao, Kaihu
Pan, Chao
Wang, Hengliang
author_facet Xu, Han
Huang, Jing
Liu, Zhaolu
Li, Xin
Wang, Kangfeng
Feng, Erling
Wu, Jun
Zhu, Li
Yao, Kaihu
Pan, Chao
Wang, Hengliang
author_sort Xu, Han
collection PubMed
description Pertussis is an acute respiratory tract infection caused by Bordetella pertussis. Even though its current vaccine coverage is relatively broad, they still have some shortcomings such as short protection time and might be incapable of blocking the spread of the disease. In this study, we developed new pertussis vaccine candidates by separately fusing three pertussis antigens (B. pertussis fimbriae 2 “Fim2”, pertussis toxin S1 subunit “PtxS1”, and filamentous hemagglutinin “FHA(1877–2250)”) to each of two immune-boosting carrier proteins (B subunits of AB5 toxin family: cholera toxin B subunit “CTB” and shiga toxin B subunit “StxB”). We then immunized mice with these fusion antigens and found that they significantly increased the serum antibody titers and elicited high bactericidal activity against B. pertussis. After CTB-or StxB-fused antigen-immunized mice were challenged with a non-lethal dose of B. pertussis, the bacterial loads in different tissues of these mice were significantly reduced, and their lung damage was nearly invisible. Furthermore, we also demonstrated that these candidate vaccines could provide strong prophylactic effects against a lethal challenge with B. pertussis. Overall, our candidate vaccines conferred better immune protection to mice compared with pertussis antigen alone. This B5 subunit-based vaccine strategy provides a promising option for vaccine design.
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spelling pubmed-82243802021-06-25 Expression of Bordetella pertussis Antigens Fused to Different Vectors and Their Effectiveness as Vaccines Xu, Han Huang, Jing Liu, Zhaolu Li, Xin Wang, Kangfeng Feng, Erling Wu, Jun Zhu, Li Yao, Kaihu Pan, Chao Wang, Hengliang Vaccines (Basel) Article Pertussis is an acute respiratory tract infection caused by Bordetella pertussis. Even though its current vaccine coverage is relatively broad, they still have some shortcomings such as short protection time and might be incapable of blocking the spread of the disease. In this study, we developed new pertussis vaccine candidates by separately fusing three pertussis antigens (B. pertussis fimbriae 2 “Fim2”, pertussis toxin S1 subunit “PtxS1”, and filamentous hemagglutinin “FHA(1877–2250)”) to each of two immune-boosting carrier proteins (B subunits of AB5 toxin family: cholera toxin B subunit “CTB” and shiga toxin B subunit “StxB”). We then immunized mice with these fusion antigens and found that they significantly increased the serum antibody titers and elicited high bactericidal activity against B. pertussis. After CTB-or StxB-fused antigen-immunized mice were challenged with a non-lethal dose of B. pertussis, the bacterial loads in different tissues of these mice were significantly reduced, and their lung damage was nearly invisible. Furthermore, we also demonstrated that these candidate vaccines could provide strong prophylactic effects against a lethal challenge with B. pertussis. Overall, our candidate vaccines conferred better immune protection to mice compared with pertussis antigen alone. This B5 subunit-based vaccine strategy provides a promising option for vaccine design. MDPI 2021-05-21 /pmc/articles/PMC8224380/ /pubmed/34064136 http://dx.doi.org/10.3390/vaccines9060542 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Han
Huang, Jing
Liu, Zhaolu
Li, Xin
Wang, Kangfeng
Feng, Erling
Wu, Jun
Zhu, Li
Yao, Kaihu
Pan, Chao
Wang, Hengliang
Expression of Bordetella pertussis Antigens Fused to Different Vectors and Their Effectiveness as Vaccines
title Expression of Bordetella pertussis Antigens Fused to Different Vectors and Their Effectiveness as Vaccines
title_full Expression of Bordetella pertussis Antigens Fused to Different Vectors and Their Effectiveness as Vaccines
title_fullStr Expression of Bordetella pertussis Antigens Fused to Different Vectors and Their Effectiveness as Vaccines
title_full_unstemmed Expression of Bordetella pertussis Antigens Fused to Different Vectors and Their Effectiveness as Vaccines
title_short Expression of Bordetella pertussis Antigens Fused to Different Vectors and Their Effectiveness as Vaccines
title_sort expression of bordetella pertussis antigens fused to different vectors and their effectiveness as vaccines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224380/
https://www.ncbi.nlm.nih.gov/pubmed/34064136
http://dx.doi.org/10.3390/vaccines9060542
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