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Global Transcriptional Response of Aspergillus niger to Blocked Active Citrate Export through Deletion of the Exporter Gene

Aspergillus niger is the major industrial citrate producer worldwide. Export as well as uptake of citric acid are believed to occur by active, proton-dependent, symport systems. Both are major bottlenecks for industrial citrate production. Therefore, we assessed the consequences of deleting the citT...

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Autores principales: Laothanachareon, Thanaporn, Bruinsma, Lyon, Nijsse, Bart, Schonewille, Tom, Suarez-Diez, Maria, Tamayo-Ramos, Juan Antonio, Martins dos Santos, Vitor A. P., Schaap, Peter J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224569/
https://www.ncbi.nlm.nih.gov/pubmed/34071072
http://dx.doi.org/10.3390/jof7060409
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author Laothanachareon, Thanaporn
Bruinsma, Lyon
Nijsse, Bart
Schonewille, Tom
Suarez-Diez, Maria
Tamayo-Ramos, Juan Antonio
Martins dos Santos, Vitor A. P.
Schaap, Peter J.
author_facet Laothanachareon, Thanaporn
Bruinsma, Lyon
Nijsse, Bart
Schonewille, Tom
Suarez-Diez, Maria
Tamayo-Ramos, Juan Antonio
Martins dos Santos, Vitor A. P.
Schaap, Peter J.
author_sort Laothanachareon, Thanaporn
collection PubMed
description Aspergillus niger is the major industrial citrate producer worldwide. Export as well as uptake of citric acid are believed to occur by active, proton-dependent, symport systems. Both are major bottlenecks for industrial citrate production. Therefore, we assessed the consequences of deleting the citT gene encoding the A. niger citrate exporter, effectively blocking active citrate export. We followed the consumption of glucose and citrate as carbon sources, monitored the secretion of organic acids and carried out a thorough transcriptome pathway enrichment analysis. Under controlled cultivation conditions that normally promote citrate secretion, the knock-out strain secreted negligible amounts of citrate. Blocking active citrate export in this way led to a reduced glucose uptake and a reduced expression of high-affinity glucose transporter genes, mstG and mstH. The glyoxylate shunt was strongly activated and an increased expression of the OAH gene was observed, resulting in a more than two-fold higher concentration of oxalate in the medium. Deletion of citT did not affect citrate uptake suggesting that citrate export and citrate uptake are uncoupled from the system.
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spelling pubmed-82245692021-06-25 Global Transcriptional Response of Aspergillus niger to Blocked Active Citrate Export through Deletion of the Exporter Gene Laothanachareon, Thanaporn Bruinsma, Lyon Nijsse, Bart Schonewille, Tom Suarez-Diez, Maria Tamayo-Ramos, Juan Antonio Martins dos Santos, Vitor A. P. Schaap, Peter J. J Fungi (Basel) Article Aspergillus niger is the major industrial citrate producer worldwide. Export as well as uptake of citric acid are believed to occur by active, proton-dependent, symport systems. Both are major bottlenecks for industrial citrate production. Therefore, we assessed the consequences of deleting the citT gene encoding the A. niger citrate exporter, effectively blocking active citrate export. We followed the consumption of glucose and citrate as carbon sources, monitored the secretion of organic acids and carried out a thorough transcriptome pathway enrichment analysis. Under controlled cultivation conditions that normally promote citrate secretion, the knock-out strain secreted negligible amounts of citrate. Blocking active citrate export in this way led to a reduced glucose uptake and a reduced expression of high-affinity glucose transporter genes, mstG and mstH. The glyoxylate shunt was strongly activated and an increased expression of the OAH gene was observed, resulting in a more than two-fold higher concentration of oxalate in the medium. Deletion of citT did not affect citrate uptake suggesting that citrate export and citrate uptake are uncoupled from the system. MDPI 2021-05-23 /pmc/articles/PMC8224569/ /pubmed/34071072 http://dx.doi.org/10.3390/jof7060409 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Laothanachareon, Thanaporn
Bruinsma, Lyon
Nijsse, Bart
Schonewille, Tom
Suarez-Diez, Maria
Tamayo-Ramos, Juan Antonio
Martins dos Santos, Vitor A. P.
Schaap, Peter J.
Global Transcriptional Response of Aspergillus niger to Blocked Active Citrate Export through Deletion of the Exporter Gene
title Global Transcriptional Response of Aspergillus niger to Blocked Active Citrate Export through Deletion of the Exporter Gene
title_full Global Transcriptional Response of Aspergillus niger to Blocked Active Citrate Export through Deletion of the Exporter Gene
title_fullStr Global Transcriptional Response of Aspergillus niger to Blocked Active Citrate Export through Deletion of the Exporter Gene
title_full_unstemmed Global Transcriptional Response of Aspergillus niger to Blocked Active Citrate Export through Deletion of the Exporter Gene
title_short Global Transcriptional Response of Aspergillus niger to Blocked Active Citrate Export through Deletion of the Exporter Gene
title_sort global transcriptional response of aspergillus niger to blocked active citrate export through deletion of the exporter gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224569/
https://www.ncbi.nlm.nih.gov/pubmed/34071072
http://dx.doi.org/10.3390/jof7060409
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