Cargando…
Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae
Zebrafish embryos and larvae are vertebrate models increasingly used in translational neuroscience research. Behavioral impairment induced by the exposure to neuroactive or neurotoxic compounds is commonly linked to changes in modulatory neurotransmitters in the brain. Although different analytical...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224676/ https://www.ncbi.nlm.nih.gov/pubmed/34071101 http://dx.doi.org/10.3390/toxics9060116 |
_version_ | 1783711938645065728 |
---|---|
author | Bellot, Marina Bartolomé, Helena Faria, Melissa Gómez-Canela, Cristian Raldúa, Demetrio |
author_facet | Bellot, Marina Bartolomé, Helena Faria, Melissa Gómez-Canela, Cristian Raldúa, Demetrio |
author_sort | Bellot, Marina |
collection | PubMed |
description | Zebrafish embryos and larvae are vertebrate models increasingly used in translational neuroscience research. Behavioral impairment induced by the exposure to neuroactive or neurotoxic compounds is commonly linked to changes in modulatory neurotransmitters in the brain. Although different analytical methods for determining monoaminergic neurochemicals in zebrafish larvae have been developed, these methods have been used only on whole larvae, as the dissection of the brain of hundreds of larvae is not feasible. This raises a key question: Are the changes in the monoaminergic profile of the whole larvae predictive of the changes in the brain? In this study, the levels of ten monoaminergic neurotransmitters were determined in the head, trunk, and the whole body of zebrafish larvae in a control group and in those treated for 24 h with 5 M deprenyl, a prototypic monoamine-oxidase B inhibitor, eight days post-fertilization. In control larvae, most of the monoaminergic neurochemicals were found at higher levels in the head than in the trunk. Significant changes were found in the distribution of some neurochemicals after deprenyl-treatment, with serotonin and norepinephrine increasing in both the head and the trunk, whereas dopamine, L-DOPA, and homovanillic acid levels were only modulated in the head. In fact, the highly significant increase in dopamine levels observed in the head after deprenyl-treatment was not detected in the whole-body analysis. These results indicate that the analysis of neurotransmitters in the zebrafish larvae whole-body should not be used as a general surrogate of the brain. |
format | Online Article Text |
id | pubmed-8224676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82246762021-06-25 Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae Bellot, Marina Bartolomé, Helena Faria, Melissa Gómez-Canela, Cristian Raldúa, Demetrio Toxics Article Zebrafish embryos and larvae are vertebrate models increasingly used in translational neuroscience research. Behavioral impairment induced by the exposure to neuroactive or neurotoxic compounds is commonly linked to changes in modulatory neurotransmitters in the brain. Although different analytical methods for determining monoaminergic neurochemicals in zebrafish larvae have been developed, these methods have been used only on whole larvae, as the dissection of the brain of hundreds of larvae is not feasible. This raises a key question: Are the changes in the monoaminergic profile of the whole larvae predictive of the changes in the brain? In this study, the levels of ten monoaminergic neurotransmitters were determined in the head, trunk, and the whole body of zebrafish larvae in a control group and in those treated for 24 h with 5 M deprenyl, a prototypic monoamine-oxidase B inhibitor, eight days post-fertilization. In control larvae, most of the monoaminergic neurochemicals were found at higher levels in the head than in the trunk. Significant changes were found in the distribution of some neurochemicals after deprenyl-treatment, with serotonin and norepinephrine increasing in both the head and the trunk, whereas dopamine, L-DOPA, and homovanillic acid levels were only modulated in the head. In fact, the highly significant increase in dopamine levels observed in the head after deprenyl-treatment was not detected in the whole-body analysis. These results indicate that the analysis of neurotransmitters in the zebrafish larvae whole-body should not be used as a general surrogate of the brain. MDPI 2021-05-23 /pmc/articles/PMC8224676/ /pubmed/34071101 http://dx.doi.org/10.3390/toxics9060116 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bellot, Marina Bartolomé, Helena Faria, Melissa Gómez-Canela, Cristian Raldúa, Demetrio Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae |
title | Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae |
title_full | Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae |
title_fullStr | Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae |
title_full_unstemmed | Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae |
title_short | Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae |
title_sort | differential modulation of the central and peripheral monoaminergic neurochemicals by deprenyl in zebrafish larvae |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224676/ https://www.ncbi.nlm.nih.gov/pubmed/34071101 http://dx.doi.org/10.3390/toxics9060116 |
work_keys_str_mv | AT bellotmarina differentialmodulationofthecentralandperipheralmonoaminergicneurochemicalsbydeprenylinzebrafishlarvae AT bartolomehelena differentialmodulationofthecentralandperipheralmonoaminergicneurochemicalsbydeprenylinzebrafishlarvae AT fariamelissa differentialmodulationofthecentralandperipheralmonoaminergicneurochemicalsbydeprenylinzebrafishlarvae AT gomezcanelacristian differentialmodulationofthecentralandperipheralmonoaminergicneurochemicalsbydeprenylinzebrafishlarvae AT ralduademetrio differentialmodulationofthecentralandperipheralmonoaminergicneurochemicalsbydeprenylinzebrafishlarvae |