Cargando…

Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae

Zebrafish embryos and larvae are vertebrate models increasingly used in translational neuroscience research. Behavioral impairment induced by the exposure to neuroactive or neurotoxic compounds is commonly linked to changes in modulatory neurotransmitters in the brain. Although different analytical...

Descripción completa

Detalles Bibliográficos
Autores principales: Bellot, Marina, Bartolomé, Helena, Faria, Melissa, Gómez-Canela, Cristian, Raldúa, Demetrio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224676/
https://www.ncbi.nlm.nih.gov/pubmed/34071101
http://dx.doi.org/10.3390/toxics9060116
_version_ 1783711938645065728
author Bellot, Marina
Bartolomé, Helena
Faria, Melissa
Gómez-Canela, Cristian
Raldúa, Demetrio
author_facet Bellot, Marina
Bartolomé, Helena
Faria, Melissa
Gómez-Canela, Cristian
Raldúa, Demetrio
author_sort Bellot, Marina
collection PubMed
description Zebrafish embryos and larvae are vertebrate models increasingly used in translational neuroscience research. Behavioral impairment induced by the exposure to neuroactive or neurotoxic compounds is commonly linked to changes in modulatory neurotransmitters in the brain. Although different analytical methods for determining monoaminergic neurochemicals in zebrafish larvae have been developed, these methods have been used only on whole larvae, as the dissection of the brain of hundreds of larvae is not feasible. This raises a key question: Are the changes in the monoaminergic profile of the whole larvae predictive of the changes in the brain? In this study, the levels of ten monoaminergic neurotransmitters were determined in the head, trunk, and the whole body of zebrafish larvae in a control group and in those treated for 24 h with 5 M deprenyl, a prototypic monoamine-oxidase B inhibitor, eight days post-fertilization. In control larvae, most of the monoaminergic neurochemicals were found at higher levels in the head than in the trunk. Significant changes were found in the distribution of some neurochemicals after deprenyl-treatment, with serotonin and norepinephrine increasing in both the head and the trunk, whereas dopamine, L-DOPA, and homovanillic acid levels were only modulated in the head. In fact, the highly significant increase in dopamine levels observed in the head after deprenyl-treatment was not detected in the whole-body analysis. These results indicate that the analysis of neurotransmitters in the zebrafish larvae whole-body should not be used as a general surrogate of the brain.
format Online
Article
Text
id pubmed-8224676
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-82246762021-06-25 Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae Bellot, Marina Bartolomé, Helena Faria, Melissa Gómez-Canela, Cristian Raldúa, Demetrio Toxics Article Zebrafish embryos and larvae are vertebrate models increasingly used in translational neuroscience research. Behavioral impairment induced by the exposure to neuroactive or neurotoxic compounds is commonly linked to changes in modulatory neurotransmitters in the brain. Although different analytical methods for determining monoaminergic neurochemicals in zebrafish larvae have been developed, these methods have been used only on whole larvae, as the dissection of the brain of hundreds of larvae is not feasible. This raises a key question: Are the changes in the monoaminergic profile of the whole larvae predictive of the changes in the brain? In this study, the levels of ten monoaminergic neurotransmitters were determined in the head, trunk, and the whole body of zebrafish larvae in a control group and in those treated for 24 h with 5 M deprenyl, a prototypic monoamine-oxidase B inhibitor, eight days post-fertilization. In control larvae, most of the monoaminergic neurochemicals were found at higher levels in the head than in the trunk. Significant changes were found in the distribution of some neurochemicals after deprenyl-treatment, with serotonin and norepinephrine increasing in both the head and the trunk, whereas dopamine, L-DOPA, and homovanillic acid levels were only modulated in the head. In fact, the highly significant increase in dopamine levels observed in the head after deprenyl-treatment was not detected in the whole-body analysis. These results indicate that the analysis of neurotransmitters in the zebrafish larvae whole-body should not be used as a general surrogate of the brain. MDPI 2021-05-23 /pmc/articles/PMC8224676/ /pubmed/34071101 http://dx.doi.org/10.3390/toxics9060116 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bellot, Marina
Bartolomé, Helena
Faria, Melissa
Gómez-Canela, Cristian
Raldúa, Demetrio
Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae
title Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae
title_full Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae
title_fullStr Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae
title_full_unstemmed Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae
title_short Differential Modulation of the Central and Peripheral Monoaminergic Neurochemicals by Deprenyl in Zebrafish Larvae
title_sort differential modulation of the central and peripheral monoaminergic neurochemicals by deprenyl in zebrafish larvae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224676/
https://www.ncbi.nlm.nih.gov/pubmed/34071101
http://dx.doi.org/10.3390/toxics9060116
work_keys_str_mv AT bellotmarina differentialmodulationofthecentralandperipheralmonoaminergicneurochemicalsbydeprenylinzebrafishlarvae
AT bartolomehelena differentialmodulationofthecentralandperipheralmonoaminergicneurochemicalsbydeprenylinzebrafishlarvae
AT fariamelissa differentialmodulationofthecentralandperipheralmonoaminergicneurochemicalsbydeprenylinzebrafishlarvae
AT gomezcanelacristian differentialmodulationofthecentralandperipheralmonoaminergicneurochemicalsbydeprenylinzebrafishlarvae
AT ralduademetrio differentialmodulationofthecentralandperipheralmonoaminergicneurochemicalsbydeprenylinzebrafishlarvae