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Effect of the electric field at 50 Hz and variable intensities on biochemical markers in the honey bee’s hemolymph

The amount of artificial electromagnetic fields of various parameters in the honey bee’s environment increases globally. So far, it had been proven that exposure to an E-field at 50 Hz can cause changes in bee’s behavior, alter the activity of proteases, and enzymatic antioxidants. Due to the potent...

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Autores principales: Migdał, Paweł, Murawska, Agnieszka, Bieńkowski, Paweł, Strachecka, Aneta, Roman, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224844/
https://www.ncbi.nlm.nih.gov/pubmed/34166412
http://dx.doi.org/10.1371/journal.pone.0252858
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author Migdał, Paweł
Murawska, Agnieszka
Bieńkowski, Paweł
Strachecka, Aneta
Roman, Adam
author_facet Migdał, Paweł
Murawska, Agnieszka
Bieńkowski, Paweł
Strachecka, Aneta
Roman, Adam
author_sort Migdał, Paweł
collection PubMed
description The amount of artificial electromagnetic fields of various parameters in the honey bee’s environment increases globally. So far, it had been proven that exposure to an E-field at 50 Hz can cause changes in bee’s behavior, alter the activity of proteases, and enzymatic antioxidants. Due to the potentially harmful effect of this factor on honey bees, we decided to investigate the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP), and the concentration of albumin and creatinine in bee’s hemolymph after exposure to 50 Hz E-field. Honey bee workers were placed in wooden cages (200 × 150 × 70 mm) and exposed to the 50 Hz E-field with the intensity of <1, 5.0, 11.5, 23.0, or 34.5 kV/m for 1, 3, 6, or 12h. A homogeneous 50 Hz E-field was generated in the form of a plate capacitor. Hemolymph samples for analysis were taken immediately after the end of exposure to the E-field from 100 bees from each group. According to our study, the activity of AST, ALT, and ALP in honey bees’ hemolymph decreased after exposure to 50 Hz E-field with various intensities. The decrease in AST, ALT, and ALP activity intensified with prolonged exposure time. 50 Hz E-field may cause the impairment of crucial metabolic cycles in the honey bees’ organism (such as the citric acid cycle, ATP synthesis, oxidative phosphorylation, β-oxidation). Moreover, exposure to E-Field altered the concentration of creatinine and albumin, which are important non-enzymatic antioxidants. Such changes may indicate a disturbance in protein metabolism and increased muscle activity.
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spelling pubmed-82248442021-07-19 Effect of the electric field at 50 Hz and variable intensities on biochemical markers in the honey bee’s hemolymph Migdał, Paweł Murawska, Agnieszka Bieńkowski, Paweł Strachecka, Aneta Roman, Adam PLoS One Research Article The amount of artificial electromagnetic fields of various parameters in the honey bee’s environment increases globally. So far, it had been proven that exposure to an E-field at 50 Hz can cause changes in bee’s behavior, alter the activity of proteases, and enzymatic antioxidants. Due to the potentially harmful effect of this factor on honey bees, we decided to investigate the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP), and the concentration of albumin and creatinine in bee’s hemolymph after exposure to 50 Hz E-field. Honey bee workers were placed in wooden cages (200 × 150 × 70 mm) and exposed to the 50 Hz E-field with the intensity of <1, 5.0, 11.5, 23.0, or 34.5 kV/m for 1, 3, 6, or 12h. A homogeneous 50 Hz E-field was generated in the form of a plate capacitor. Hemolymph samples for analysis were taken immediately after the end of exposure to the E-field from 100 bees from each group. According to our study, the activity of AST, ALT, and ALP in honey bees’ hemolymph decreased after exposure to 50 Hz E-field with various intensities. The decrease in AST, ALT, and ALP activity intensified with prolonged exposure time. 50 Hz E-field may cause the impairment of crucial metabolic cycles in the honey bees’ organism (such as the citric acid cycle, ATP synthesis, oxidative phosphorylation, β-oxidation). Moreover, exposure to E-Field altered the concentration of creatinine and albumin, which are important non-enzymatic antioxidants. Such changes may indicate a disturbance in protein metabolism and increased muscle activity. Public Library of Science 2021-06-24 /pmc/articles/PMC8224844/ /pubmed/34166412 http://dx.doi.org/10.1371/journal.pone.0252858 Text en © 2021 Migdał et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Migdał, Paweł
Murawska, Agnieszka
Bieńkowski, Paweł
Strachecka, Aneta
Roman, Adam
Effect of the electric field at 50 Hz and variable intensities on biochemical markers in the honey bee’s hemolymph
title Effect of the electric field at 50 Hz and variable intensities on biochemical markers in the honey bee’s hemolymph
title_full Effect of the electric field at 50 Hz and variable intensities on biochemical markers in the honey bee’s hemolymph
title_fullStr Effect of the electric field at 50 Hz and variable intensities on biochemical markers in the honey bee’s hemolymph
title_full_unstemmed Effect of the electric field at 50 Hz and variable intensities on biochemical markers in the honey bee’s hemolymph
title_short Effect of the electric field at 50 Hz and variable intensities on biochemical markers in the honey bee’s hemolymph
title_sort effect of the electric field at 50 hz and variable intensities on biochemical markers in the honey bee’s hemolymph
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224844/
https://www.ncbi.nlm.nih.gov/pubmed/34166412
http://dx.doi.org/10.1371/journal.pone.0252858
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