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Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients

Acinetobacter baumannii (A. baumannii) represents a global threat owing to its ability to resist most of the currently available antimicrobial agents. Moreover, emergence of carbapenem resistant A. baumannii (CR-AB) isolates limits the available treatment options. Enzymatic degradation by variety of...

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Autores principales: Hassan, Reem M., Salem, Sherifa T., Hassan, Saly Ismail Mostafa, Hegab, Asmaa Sayed, Elkholy, Yasmine S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224909/
https://www.ncbi.nlm.nih.gov/pubmed/34166384
http://dx.doi.org/10.1371/journal.pone.0251508
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author Hassan, Reem M.
Salem, Sherifa T.
Hassan, Saly Ismail Mostafa
Hegab, Asmaa Sayed
Elkholy, Yasmine S.
author_facet Hassan, Reem M.
Salem, Sherifa T.
Hassan, Saly Ismail Mostafa
Hegab, Asmaa Sayed
Elkholy, Yasmine S.
author_sort Hassan, Reem M.
collection PubMed
description Acinetobacter baumannii (A. baumannii) represents a global threat owing to its ability to resist most of the currently available antimicrobial agents. Moreover, emergence of carbapenem resistant A. baumannii (CR-AB) isolates limits the available treatment options. Enzymatic degradation by variety of ß-lactamases, have been identified as the most common mechanism of carbapenem resistance in A. baumannii. The alarming increase in the prevalence of CR-AB necessitates continuous screening and molecular characterization to appreciate the problem. The present study was performed to assess the prevalence and characterize carbapenemases among 206 CR-AB isolated from various clinical specimens collected from different intensive care units at Kasr Al-Aini Hospital. All isolates were confirmed to be A. baumannii by detection of the blaOXA-51-like gene. Molecular screening of 13 common Ambler class bla carbapenemases genes in addition to insertion sequence (IS-1) upstream OXA-23 were performed by using four sets of multiplex PCR, followed by identification using gene sequencing technology. Among the investigated genes, the prevalence of blaOXA-23, and blaOXA-58 were 77.7%, and 1.9%, respectively. The ISAba1 was detected in 10% of the blaOXA-23 positive isolates. The prevalence of metallo-β-lactamases (MBLs) studied; blaNDM-1, blaSPM, blaVIM, blaSIM-1 were 11.7%, 6.3%, 0.5%, and 0.5% respectively. One of class A; bla KPC was detected in 10.7% of the investigated isolates. blaOXA-24/40, blaIMP, blaGES, blaVEB and blaGIM were not detected in any of the studied isolates. Moreover, 18.4% of the isolates have shown to harbor two or more of the screened bla genes. We concluded that the most prevalent type of ß-lactamases genes among CR-AB isolates collected from Egyptian patients were blaOXA-23 followed by blaNDM-1 and blaKPC.
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spelling pubmed-82249092021-07-19 Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients Hassan, Reem M. Salem, Sherifa T. Hassan, Saly Ismail Mostafa Hegab, Asmaa Sayed Elkholy, Yasmine S. PLoS One Research Article Acinetobacter baumannii (A. baumannii) represents a global threat owing to its ability to resist most of the currently available antimicrobial agents. Moreover, emergence of carbapenem resistant A. baumannii (CR-AB) isolates limits the available treatment options. Enzymatic degradation by variety of ß-lactamases, have been identified as the most common mechanism of carbapenem resistance in A. baumannii. The alarming increase in the prevalence of CR-AB necessitates continuous screening and molecular characterization to appreciate the problem. The present study was performed to assess the prevalence and characterize carbapenemases among 206 CR-AB isolated from various clinical specimens collected from different intensive care units at Kasr Al-Aini Hospital. All isolates were confirmed to be A. baumannii by detection of the blaOXA-51-like gene. Molecular screening of 13 common Ambler class bla carbapenemases genes in addition to insertion sequence (IS-1) upstream OXA-23 were performed by using four sets of multiplex PCR, followed by identification using gene sequencing technology. Among the investigated genes, the prevalence of blaOXA-23, and blaOXA-58 were 77.7%, and 1.9%, respectively. The ISAba1 was detected in 10% of the blaOXA-23 positive isolates. The prevalence of metallo-β-lactamases (MBLs) studied; blaNDM-1, blaSPM, blaVIM, blaSIM-1 were 11.7%, 6.3%, 0.5%, and 0.5% respectively. One of class A; bla KPC was detected in 10.7% of the investigated isolates. blaOXA-24/40, blaIMP, blaGES, blaVEB and blaGIM were not detected in any of the studied isolates. Moreover, 18.4% of the isolates have shown to harbor two or more of the screened bla genes. We concluded that the most prevalent type of ß-lactamases genes among CR-AB isolates collected from Egyptian patients were blaOXA-23 followed by blaNDM-1 and blaKPC. Public Library of Science 2021-06-24 /pmc/articles/PMC8224909/ /pubmed/34166384 http://dx.doi.org/10.1371/journal.pone.0251508 Text en © 2021 Hassan et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hassan, Reem M.
Salem, Sherifa T.
Hassan, Saly Ismail Mostafa
Hegab, Asmaa Sayed
Elkholy, Yasmine S.
Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients
title Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients
title_full Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients
title_fullStr Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients
title_full_unstemmed Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients
title_short Molecular characterization of carbapenem-resistant Acinetobacter baumannii clinical isolates from Egyptian patients
title_sort molecular characterization of carbapenem-resistant acinetobacter baumannii clinical isolates from egyptian patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224909/
https://www.ncbi.nlm.nih.gov/pubmed/34166384
http://dx.doi.org/10.1371/journal.pone.0251508
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