Successful Use of Geochemical Tools to Trace the Geographic Origin of Long-Snouted Seahorse Hippocampus guttulatus Raised in Captivity

SIMPLE SUMMARY: Seahorses (Hippocampus spp.) are currently exposed to a multitude of anthropogenic pressures worldwide. The illegal, unreported, and unregulated (IUU) fisheries and trade of these flagship species undermine the efforts to manage and protect their wild populations. Here we aim to validate a forensic tool to identify the geographic origin of seahorses and contribute to the ongoing fight against the illegal capture and trade of these organisms. The elemental fingerprints of long-snouted seahorse (Hippocampus guttulatus) bony structures, including the subdermal bony plates that cover their body, revealed that they can be successfully employed to confirm their geographic origin. The results of this first study using seahorses raised in captivity indicate that this tool may also allow to discriminate between different populations of wild specimens and enhance the traceability of traded specimens. ABSTRACT: The global market of dried seahorses mainly supplies Traditional Chinese Medicine and still relies on blurry trade chains that often cover less sustainable practices targeting these pricey and endangered fish. As such, reliable tools that allow the enforcement of traceability, namely to confirm the geographic origin of traded seahorses, are urgently needed. The present study evaluated the use of elemental fingerprints (EF) in the bony structures of long-snouted seahorses Hippocampus guttulatus raised in captivity in two different locations (southern Portugal and Northern Spain) to discriminate their geographic origin. The EF of different body parts of H. guttulatus were also evaluated as potential proxies for the EF of the whole body, in order to allow the analysis of damaged specimens and avoid the use of whole specimens for analysis. The contrasting EF of H. guttulatus raised in the two locations allowed their reliable discrimination. Although no single body part exactly mimicked the EF of the whole body, seahorse trunks, as well as damaged specimens, could still be correctly allocated to their geographic origin. This promising forensic approach to discriminate the geographic origin of seahorses raised in captivity should now be validated for wild conspecifics originating from different locations, as well as for other species within genus Hippocampus.