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Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2

In vitro studies of a disease are key to any in vivo investigation in understanding the disease and developing new therapy regimens. Immortalized cancer cell lines are the best and easiest model for studying cancer in vitro. Here, we report the establishment of a naturally immortalized highly tumori...

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Autores principales: Ali, Rizwan, Al Zahrani, Hajar, Barhoumi, Tlili, Alhallaj, Alshaimaa, Mashhour, Abdullah, Alshammari, Musaad A., Alshawakir, Yasser A., Baz, Omar, Alanazi, Abdullah H., Khan, Abdul Latif, Al Nikhli, Hassan, Al Balwi, Mohammed A., Al Riyees, Lolwah, Boudjelal, Mohamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225085/
https://www.ncbi.nlm.nih.gov/pubmed/34073849
http://dx.doi.org/10.3390/cells10061303
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author Ali, Rizwan
Al Zahrani, Hajar
Barhoumi, Tlili
Alhallaj, Alshaimaa
Mashhour, Abdullah
Alshammari, Musaad A.
Alshawakir, Yasser A.
Baz, Omar
Alanazi, Abdullah H.
Khan, Abdul Latif
Al Nikhli, Hassan
Al Balwi, Mohammed A.
Al Riyees, Lolwah
Boudjelal, Mohamed
author_facet Ali, Rizwan
Al Zahrani, Hajar
Barhoumi, Tlili
Alhallaj, Alshaimaa
Mashhour, Abdullah
Alshammari, Musaad A.
Alshawakir, Yasser A.
Baz, Omar
Alanazi, Abdullah H.
Khan, Abdul Latif
Al Nikhli, Hassan
Al Balwi, Mohammed A.
Al Riyees, Lolwah
Boudjelal, Mohamed
author_sort Ali, Rizwan
collection PubMed
description In vitro studies of a disease are key to any in vivo investigation in understanding the disease and developing new therapy regimens. Immortalized cancer cell lines are the best and easiest model for studying cancer in vitro. Here, we report the establishment of a naturally immortalized highly tumorigenic and triple-negative breast cancer cell line, KAIMRC2. This cell line is derived from a Saudi Arabian female breast cancer patient with invasive ductal carcinoma. Immunocytochemistry showed a significant ratio of the KAIMRC2 cells’ expressing key breast epithelial and cancer stem cells (CSCs) markers, including CD47, CD133, CD49f, CD44, and ALDH-1A1. Gene and protein expression analysis showed overexpression of ABC transporter and AKT-PI3Kinase as well as JAK/STAT signaling pathways. In contrast, the absence of the tumor suppressor genes p53 and p73 may explain their high proliferative index. The mice model also confirmed the tumorigenic potential of the KAIMRC2 cell line, and drug tolerance studies revealed few very potent candidates. Our results confirmed an aggressive phenotype with metastatic potential and cancer stem cell-like characteristics of the KAIMR2 cell line. Furthermore, we have also presented potent small molecule inhibitors, especially Ryuvidine, that can be further developed, alone or in synergy with other potent inhibitors, to target multiple cancer-related pathways.
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spelling pubmed-82250852021-06-25 Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2 Ali, Rizwan Al Zahrani, Hajar Barhoumi, Tlili Alhallaj, Alshaimaa Mashhour, Abdullah Alshammari, Musaad A. Alshawakir, Yasser A. Baz, Omar Alanazi, Abdullah H. Khan, Abdul Latif Al Nikhli, Hassan Al Balwi, Mohammed A. Al Riyees, Lolwah Boudjelal, Mohamed Cells Article In vitro studies of a disease are key to any in vivo investigation in understanding the disease and developing new therapy regimens. Immortalized cancer cell lines are the best and easiest model for studying cancer in vitro. Here, we report the establishment of a naturally immortalized highly tumorigenic and triple-negative breast cancer cell line, KAIMRC2. This cell line is derived from a Saudi Arabian female breast cancer patient with invasive ductal carcinoma. Immunocytochemistry showed a significant ratio of the KAIMRC2 cells’ expressing key breast epithelial and cancer stem cells (CSCs) markers, including CD47, CD133, CD49f, CD44, and ALDH-1A1. Gene and protein expression analysis showed overexpression of ABC transporter and AKT-PI3Kinase as well as JAK/STAT signaling pathways. In contrast, the absence of the tumor suppressor genes p53 and p73 may explain their high proliferative index. The mice model also confirmed the tumorigenic potential of the KAIMRC2 cell line, and drug tolerance studies revealed few very potent candidates. Our results confirmed an aggressive phenotype with metastatic potential and cancer stem cell-like characteristics of the KAIMR2 cell line. Furthermore, we have also presented potent small molecule inhibitors, especially Ryuvidine, that can be further developed, alone or in synergy with other potent inhibitors, to target multiple cancer-related pathways. MDPI 2021-05-24 /pmc/articles/PMC8225085/ /pubmed/34073849 http://dx.doi.org/10.3390/cells10061303 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ali, Rizwan
Al Zahrani, Hajar
Barhoumi, Tlili
Alhallaj, Alshaimaa
Mashhour, Abdullah
Alshammari, Musaad A.
Alshawakir, Yasser A.
Baz, Omar
Alanazi, Abdullah H.
Khan, Abdul Latif
Al Nikhli, Hassan
Al Balwi, Mohammed A.
Al Riyees, Lolwah
Boudjelal, Mohamed
Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2
title Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2
title_full Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2
title_fullStr Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2
title_full_unstemmed Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2
title_short Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2
title_sort isolation and establishment of a highly proliferative, cancer stem cell-like, and naturally immortalized triple-negative breast cancer cell line, kaimrc2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225085/
https://www.ncbi.nlm.nih.gov/pubmed/34073849
http://dx.doi.org/10.3390/cells10061303
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