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Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains

PURPOSE: The aim of this work was to analyze the complete genome of probiotic bacteria Lactobacillus plantarum 8 RA 3, Lactobacillus fermentum 90 TC-4, Lactobacillus fermentum 39, Bifidobacterium bifidum 791, Bifidobacterium bifidum 1, and Bifidobacterium longum 379 and to test their activity agains...

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Autores principales: Soloveva, Irina V., Ilyicheva, Tatyana N., Marchenko, Vasiliy Yu., Pyankov, Oleg V., Tochilina, Anna G., Belova, Irina V., Zhirnov, Vladimir A., Bormotov, Nikolay I., Skarnovich, Maksim O., Durymanov, Aleksander G., Molodtsova, Svetlana B., Filippova, Ekaterina I., Ovchinnikova, Alena S., Magerramova, Anastasia V., Ryzhikov, Alexander B., Maksyutov, Rinat A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225420/
https://www.ncbi.nlm.nih.gov/pubmed/34258278
http://dx.doi.org/10.1155/2021/6662027
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author Soloveva, Irina V.
Ilyicheva, Tatyana N.
Marchenko, Vasiliy Yu.
Pyankov, Oleg V.
Tochilina, Anna G.
Belova, Irina V.
Zhirnov, Vladimir A.
Bormotov, Nikolay I.
Skarnovich, Maksim O.
Durymanov, Aleksander G.
Molodtsova, Svetlana B.
Filippova, Ekaterina I.
Ovchinnikova, Alena S.
Magerramova, Anastasia V.
Ryzhikov, Alexander B.
Maksyutov, Rinat A.
author_facet Soloveva, Irina V.
Ilyicheva, Tatyana N.
Marchenko, Vasiliy Yu.
Pyankov, Oleg V.
Tochilina, Anna G.
Belova, Irina V.
Zhirnov, Vladimir A.
Bormotov, Nikolay I.
Skarnovich, Maksim O.
Durymanov, Aleksander G.
Molodtsova, Svetlana B.
Filippova, Ekaterina I.
Ovchinnikova, Alena S.
Magerramova, Anastasia V.
Ryzhikov, Alexander B.
Maksyutov, Rinat A.
author_sort Soloveva, Irina V.
collection PubMed
description PURPOSE: The aim of this work was to analyze the complete genome of probiotic bacteria Lactobacillus plantarum 8 RA 3, Lactobacillus fermentum 90 TC-4, Lactobacillus fermentum 39, Bifidobacterium bifidum 791, Bifidobacterium bifidum 1, and Bifidobacterium longum 379 and to test their activity against influenza A and SARS-CoV-2 viruses. METHODS: To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc. MiSeq platform. To determine the antiviral activity, A/Lipetsk/1V/2018 (H1N1 pdm09) (EPI_ISL_332798) and A/common gull/Saratov/1676/2018 (H5N6) (EPI_ISL_336925) influenza viruses and SARS-CoV-2 virus strain Australia/VIC01/2020 (GenBank: MT007544.1) were used. RESULTS: All studied probiotic bacteria are nonpathogenic for humans and do not contain the determinants of transmission-type antibiotic resistance and integrated plasmids. Resistance to antibiotics of different classes is explained by the presence of molecular efflux pumps of the MatE and MFS families. Cultures of L. fermentum 90 TC 4, L. plantarum 8 RA 3, and B. bifidum 791 showed a pronounced activity against influenza A viruses in MDCK cells. Activity against the SARS-CoV-2 virus was demonstrated only by the L. fermentum 90 TC 4 strain in VERO cells. CONCLUSIONS: The studied probiotic bacteria are safe, have antiviral activity, and are of great importance for the prevention of diseases caused by respiratory viruses that can also infect the human intestine.
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spelling pubmed-82254202021-07-12 Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains Soloveva, Irina V. Ilyicheva, Tatyana N. Marchenko, Vasiliy Yu. Pyankov, Oleg V. Tochilina, Anna G. Belova, Irina V. Zhirnov, Vladimir A. Bormotov, Nikolay I. Skarnovich, Maksim O. Durymanov, Aleksander G. Molodtsova, Svetlana B. Filippova, Ekaterina I. Ovchinnikova, Alena S. Magerramova, Anastasia V. Ryzhikov, Alexander B. Maksyutov, Rinat A. Biomed Res Int Research Article PURPOSE: The aim of this work was to analyze the complete genome of probiotic bacteria Lactobacillus plantarum 8 RA 3, Lactobacillus fermentum 90 TC-4, Lactobacillus fermentum 39, Bifidobacterium bifidum 791, Bifidobacterium bifidum 1, and Bifidobacterium longum 379 and to test their activity against influenza A and SARS-CoV-2 viruses. METHODS: To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc. MiSeq platform. To determine the antiviral activity, A/Lipetsk/1V/2018 (H1N1 pdm09) (EPI_ISL_332798) and A/common gull/Saratov/1676/2018 (H5N6) (EPI_ISL_336925) influenza viruses and SARS-CoV-2 virus strain Australia/VIC01/2020 (GenBank: MT007544.1) were used. RESULTS: All studied probiotic bacteria are nonpathogenic for humans and do not contain the determinants of transmission-type antibiotic resistance and integrated plasmids. Resistance to antibiotics of different classes is explained by the presence of molecular efflux pumps of the MatE and MFS families. Cultures of L. fermentum 90 TC 4, L. plantarum 8 RA 3, and B. bifidum 791 showed a pronounced activity against influenza A viruses in MDCK cells. Activity against the SARS-CoV-2 virus was demonstrated only by the L. fermentum 90 TC 4 strain in VERO cells. CONCLUSIONS: The studied probiotic bacteria are safe, have antiviral activity, and are of great importance for the prevention of diseases caused by respiratory viruses that can also infect the human intestine. Hindawi 2021-06-18 /pmc/articles/PMC8225420/ /pubmed/34258278 http://dx.doi.org/10.1155/2021/6662027 Text en Copyright © 2021 Irina V. Soloveva et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Soloveva, Irina V.
Ilyicheva, Tatyana N.
Marchenko, Vasiliy Yu.
Pyankov, Oleg V.
Tochilina, Anna G.
Belova, Irina V.
Zhirnov, Vladimir A.
Bormotov, Nikolay I.
Skarnovich, Maksim O.
Durymanov, Aleksander G.
Molodtsova, Svetlana B.
Filippova, Ekaterina I.
Ovchinnikova, Alena S.
Magerramova, Anastasia V.
Ryzhikov, Alexander B.
Maksyutov, Rinat A.
Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains
title Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains
title_full Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains
title_fullStr Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains
title_full_unstemmed Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains
title_short Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains
title_sort genome features and in vitro activity against influenza a and sars-cov-2 viruses of six probiotic strains
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225420/
https://www.ncbi.nlm.nih.gov/pubmed/34258278
http://dx.doi.org/10.1155/2021/6662027
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