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Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities

Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK[CE]821), is...

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Autores principales: de Melo Pacífico, Dvison, Costa, Cecília Leite, Moura, Hercules, Barr, John R., Maia, Guilherme Augusto, Filho, Vilmar Benetti, Moreira, Renato Simões, Wagner, Glauber, Domingues, Regina Maria Cavalcanti Pilotto, Quesada-Gómez, Carlos, de Oliveira Ferreira, Eliane, de Castro Brito, Gerly Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225638/
https://www.ncbi.nlm.nih.gov/pubmed/34168208
http://dx.doi.org/10.1038/s41598-021-92684-0
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author de Melo Pacífico, Dvison
Costa, Cecília Leite
Moura, Hercules
Barr, John R.
Maia, Guilherme Augusto
Filho, Vilmar Benetti
Moreira, Renato Simões
Wagner, Glauber
Domingues, Regina Maria Cavalcanti Pilotto
Quesada-Gómez, Carlos
de Oliveira Ferreira, Eliane
de Castro Brito, Gerly Anne
author_facet de Melo Pacífico, Dvison
Costa, Cecília Leite
Moura, Hercules
Barr, John R.
Maia, Guilherme Augusto
Filho, Vilmar Benetti
Moreira, Renato Simões
Wagner, Glauber
Domingues, Regina Maria Cavalcanti Pilotto
Quesada-Gómez, Carlos
de Oliveira Ferreira, Eliane
de Castro Brito, Gerly Anne
author_sort de Melo Pacífico, Dvison
collection PubMed
description Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK[CE]821), isolated in Brazil, and NAP1/027/ST01 (LIBA5756), isolated during a 2010 outbreak in Costa Rica. C. difficile isolates were cultured and extracellular proteins were analyzed using high-performance liquid chromatography-tandem mass spectrometry. Genomic analysis revealed that these isolates shared most of the gene composition. Only 83 and 290 NAP1/027 genes were considered singletons in ICC-45 and NAP1/027, respectively. Exoproteome analysis revealed 197 proteins, of which 192 were similar in both strains. Only five proteins were exclusive to the ICC-45 strain. These proteins were involved with catalytic and binding functions and indirectly interacted with proteins related to pathogenicity. Most proteins, including TcdA, TcdB, flagellin subunit, and cell surface protein, were overrepresented in the ICC-45 strain; 14 proteins, including mature S-layer protein, were present in higher proportions in LIBA5756. Data are available via ProteomeXchange with identifier PXD026218. These data show close similarity between the genome and proteins in the supernatant of two strains with hypervirulent features isolated in Latin America and underscore the importance of epidemiological surveillance of the transmission and emergence of new strains.
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spelling pubmed-82256382021-07-02 Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities de Melo Pacífico, Dvison Costa, Cecília Leite Moura, Hercules Barr, John R. Maia, Guilherme Augusto Filho, Vilmar Benetti Moreira, Renato Simões Wagner, Glauber Domingues, Regina Maria Cavalcanti Pilotto Quesada-Gómez, Carlos de Oliveira Ferreira, Eliane de Castro Brito, Gerly Anne Sci Rep Article Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK[CE]821), isolated in Brazil, and NAP1/027/ST01 (LIBA5756), isolated during a 2010 outbreak in Costa Rica. C. difficile isolates were cultured and extracellular proteins were analyzed using high-performance liquid chromatography-tandem mass spectrometry. Genomic analysis revealed that these isolates shared most of the gene composition. Only 83 and 290 NAP1/027 genes were considered singletons in ICC-45 and NAP1/027, respectively. Exoproteome analysis revealed 197 proteins, of which 192 were similar in both strains. Only five proteins were exclusive to the ICC-45 strain. These proteins were involved with catalytic and binding functions and indirectly interacted with proteins related to pathogenicity. Most proteins, including TcdA, TcdB, flagellin subunit, and cell surface protein, were overrepresented in the ICC-45 strain; 14 proteins, including mature S-layer protein, were present in higher proportions in LIBA5756. Data are available via ProteomeXchange with identifier PXD026218. These data show close similarity between the genome and proteins in the supernatant of two strains with hypervirulent features isolated in Latin America and underscore the importance of epidemiological surveillance of the transmission and emergence of new strains. Nature Publishing Group UK 2021-06-24 /pmc/articles/PMC8225638/ /pubmed/34168208 http://dx.doi.org/10.1038/s41598-021-92684-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
de Melo Pacífico, Dvison
Costa, Cecília Leite
Moura, Hercules
Barr, John R.
Maia, Guilherme Augusto
Filho, Vilmar Benetti
Moreira, Renato Simões
Wagner, Glauber
Domingues, Regina Maria Cavalcanti Pilotto
Quesada-Gómez, Carlos
de Oliveira Ferreira, Eliane
de Castro Brito, Gerly Anne
Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities
title Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities
title_full Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities
title_fullStr Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities
title_full_unstemmed Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities
title_short Exoproteomic analysis of two MLST clade 2 strains of Clostridioides difficile from Latin America reveal close similarities
title_sort exoproteomic analysis of two mlst clade 2 strains of clostridioides difficile from latin america reveal close similarities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225638/
https://www.ncbi.nlm.nih.gov/pubmed/34168208
http://dx.doi.org/10.1038/s41598-021-92684-0
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