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A protocol for detecting elemental calcium signals (Ca(2+) puffs) in mammalian cells using total internal reflection fluorescence microscopy

This protocol outlines steps to visualize and detect Ca(2+) puffs following photo-liberation of caged inositol-1,4,5-trisphosphate (IP(3)) from HEK-293 cells expressing only the native IP(3)R type 1 receptor using total internal reflection fluorescence (TIRF) microscopy. TIRF microscopy offers high...

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Detalles Bibliográficos
Autores principales:	Arige, Vikas, Emrich, Scott M., Yoast, Ryan E., Trebak, Mohamed, Yule, David I.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225975/ https://www.ncbi.nlm.nih.gov/pubmed/34195673 http://dx.doi.org/10.1016/j.xpro.2021.100618

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