An optimized confocal intravital microscopy protocol for long-term live imaging of murine F-actin organization during naïve lymphocyte migration

Actin plays a crucial role during cell motility, but the organization of F-actin filaments during lymphocyte migration has not been visualized in vivo. Here, we present a 4D imaging platform using high-resolution confocal intravital microscopy to precisely determine the F-actin filament profile duri...

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Detalles Bibliográficos
Autores principales: Yan, Serena L.S., Kehrl, John H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225983/
https://www.ncbi.nlm.nih.gov/pubmed/34195670
http://dx.doi.org/10.1016/j.xpro.2021.100498
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author Yan, Serena L.S.
Kehrl, John H.
author_facet Yan, Serena L.S.
Kehrl, John H.
author_sort Yan, Serena L.S.
collection PubMed
description Actin plays a crucial role during cell motility, but the organization of F-actin filaments during lymphocyte migration has not been visualized in vivo. Here, we present a 4D imaging platform using high-resolution confocal intravital microscopy to precisely determine the F-actin filament profile during lymphocyte transendothelial migration and interstitial migration. This protocol allows prolonged live imaging by laser scanning microscopy with advanced spatial resolution compared with the traditional multi-photon intravital microscopy techniques. For complete details on the use and execution of this protocol, please refer to Yan et al. (2019).
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spelling pubmed-82259832021-06-29 An optimized confocal intravital microscopy protocol for long-term live imaging of murine F-actin organization during naïve lymphocyte migration Yan, Serena L.S. Kehrl, John H. STAR Protoc Protocol Actin plays a crucial role during cell motility, but the organization of F-actin filaments during lymphocyte migration has not been visualized in vivo. Here, we present a 4D imaging platform using high-resolution confocal intravital microscopy to precisely determine the F-actin filament profile during lymphocyte transendothelial migration and interstitial migration. This protocol allows prolonged live imaging by laser scanning microscopy with advanced spatial resolution compared with the traditional multi-photon intravital microscopy techniques. For complete details on the use and execution of this protocol, please refer to Yan et al. (2019). Elsevier 2021-06-18 /pmc/articles/PMC8225983/ /pubmed/34195670 http://dx.doi.org/10.1016/j.xpro.2021.100498 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yan, Serena L.S.
Kehrl, John H.
An optimized confocal intravital microscopy protocol for long-term live imaging of murine F-actin organization during naïve lymphocyte migration
title An optimized confocal intravital microscopy protocol for long-term live imaging of murine F-actin organization during naïve lymphocyte migration
title_full An optimized confocal intravital microscopy protocol for long-term live imaging of murine F-actin organization during naïve lymphocyte migration
title_fullStr An optimized confocal intravital microscopy protocol for long-term live imaging of murine F-actin organization during naïve lymphocyte migration
title_full_unstemmed An optimized confocal intravital microscopy protocol for long-term live imaging of murine F-actin organization during naïve lymphocyte migration
title_short An optimized confocal intravital microscopy protocol for long-term live imaging of murine F-actin organization during naïve lymphocyte migration
title_sort optimized confocal intravital microscopy protocol for long-term live imaging of murine f-actin organization during naïve lymphocyte migration
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225983/
https://www.ncbi.nlm.nih.gov/pubmed/34195670
http://dx.doi.org/10.1016/j.xpro.2021.100498
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