Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide

Ca(2+) imaging is the most frequently used technique to study glial cell physiology. While chemical Ca(2+) indicators served to visualize and measure changes in glial cell cytosolic Ca(2+) concentration for several decades, genetically encoded Ca(2+) indicators (GECIs) have become state of the art i...

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Detalles Bibliográficos
Autores principales: Lohr, Christian, Beiersdorfer, Antonia, Fischer, Timo, Hirnet, Daniela, Rotermund, Natalie, Sauer, Jessica, Schulz, Kristina, Gee, Christine E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Cellular Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226001/
https://www.ncbi.nlm.nih.gov/pubmed/34177468
http://dx.doi.org/10.3389/fncel.2021.690147
Descripción
Sumario:Ca(2+) imaging is the most frequently used technique to study glial cell physiology. While chemical Ca(2+) indicators served to visualize and measure changes in glial cell cytosolic Ca(2+) concentration for several decades, genetically encoded Ca(2+) indicators (GECIs) have become state of the art in recent years. Great improvements have been made since the development of the first GECI and a large number of GECIs with different physical properties exist, rendering it difficult to select the optimal Ca(2+) indicator. This review discusses some of the most frequently used GECIs and their suitability for glial cell research.

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