Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide

Ca(2+) imaging is the most frequently used technique to study glial cell physiology. While chemical Ca(2+) indicators served to visualize and measure changes in glial cell cytosolic Ca(2+) concentration for several decades, genetically encoded Ca(2+) indicators (GECIs) have become state of the art i...

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Autores principales: Lohr, Christian, Beiersdorfer, Antonia, Fischer, Timo, Hirnet, Daniela, Rotermund, Natalie, Sauer, Jessica, Schulz, Kristina, Gee, Christine E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Cellular Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226001/
https://www.ncbi.nlm.nih.gov/pubmed/34177468
http://dx.doi.org/10.3389/fncel.2021.690147
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author Lohr, Christian
Beiersdorfer, Antonia
Fischer, Timo
Hirnet, Daniela
Rotermund, Natalie
Sauer, Jessica
Schulz, Kristina
Gee, Christine E.
author_facet Lohr, Christian
Beiersdorfer, Antonia
Fischer, Timo
Hirnet, Daniela
Rotermund, Natalie
Sauer, Jessica
Schulz, Kristina
Gee, Christine E.
author_sort Lohr, Christian
collection PubMed
description Ca(2+) imaging is the most frequently used technique to study glial cell physiology. While chemical Ca(2+) indicators served to visualize and measure changes in glial cell cytosolic Ca(2+) concentration for several decades, genetically encoded Ca(2+) indicators (GECIs) have become state of the art in recent years. Great improvements have been made since the development of the first GECI and a large number of GECIs with different physical properties exist, rendering it difficult to select the optimal Ca(2+) indicator. This review discusses some of the most frequently used GECIs and their suitability for glial cell research.
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spelling pubmed-82260012021-06-26 Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide Lohr, Christian Beiersdorfer, Antonia Fischer, Timo Hirnet, Daniela Rotermund, Natalie Sauer, Jessica Schulz, Kristina Gee, Christine E. Front Cell Neurosci Cellular Neuroscience Ca(2+) imaging is the most frequently used technique to study glial cell physiology. While chemical Ca(2+) indicators served to visualize and measure changes in glial cell cytosolic Ca(2+) concentration for several decades, genetically encoded Ca(2+) indicators (GECIs) have become state of the art in recent years. Great improvements have been made since the development of the first GECI and a large number of GECIs with different physical properties exist, rendering it difficult to select the optimal Ca(2+) indicator. This review discusses some of the most frequently used GECIs and their suitability for glial cell research. Frontiers Media S.A. 2021-06-11 /pmc/articles/PMC8226001/ /pubmed/34177468 http://dx.doi.org/10.3389/fncel.2021.690147 Text en Copyright © 2021 Lohr, Beiersdorfer, Fischer, Hirnet, Rotermund, Sauer, Schulz and Gee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular Neuroscience
Lohr, Christian
Beiersdorfer, Antonia
Fischer, Timo
Hirnet, Daniela
Rotermund, Natalie
Sauer, Jessica
Schulz, Kristina
Gee, Christine E.
Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide
title Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide
title_full Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide
title_fullStr Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide
title_full_unstemmed Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide
title_short Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide
title_sort using genetically encoded calcium indicators to study astrocyte physiology: a field guide
topic Cellular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226001/
https://www.ncbi.nlm.nih.gov/pubmed/34177468
http://dx.doi.org/10.3389/fncel.2021.690147
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