Methods for in vitro CRISPR/CasRx-Mediated RNA Editing

Specific changes in the genome have been accomplished by the revolutionary gene-editing tool known as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. The advent of programmable RNA editing CRISPR/Cas nucleases has made this gene-editing tool safer a...

Descripción completa

Detalles Bibliográficos
Autores principales: Chuang, Yu-Fan, Wang, Peng-Yuan, Kumar, Satheesh, Lama, Suraj, Lin, Fan-Li, Liu, Guei-Sheung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226256/
https://www.ncbi.nlm.nih.gov/pubmed/34178991
http://dx.doi.org/10.3389/fcell.2021.667879
_version_ 1783712251251785728
author Chuang, Yu-Fan
Wang, Peng-Yuan
Kumar, Satheesh
Lama, Suraj
Lin, Fan-Li
Liu, Guei-Sheung
author_facet Chuang, Yu-Fan
Wang, Peng-Yuan
Kumar, Satheesh
Lama, Suraj
Lin, Fan-Li
Liu, Guei-Sheung
author_sort Chuang, Yu-Fan
collection PubMed
description Specific changes in the genome have been accomplished by the revolutionary gene-editing tool known as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. The advent of programmable RNA editing CRISPR/Cas nucleases has made this gene-editing tool safer and more precise. Specifically, CasRx, a family member of the Cas13d family, has shown great therapeutic potential. Here, we describe the in vitro methods of utilizing this powerful RNA editing platform and determine the RNA editing efficiencies for CasRx with different forms of guide RNAs (also known as gRNA or sgRNA).
format Online
Article
Text
id pubmed-8226256
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82262562021-06-26 Methods for in vitro CRISPR/CasRx-Mediated RNA Editing Chuang, Yu-Fan Wang, Peng-Yuan Kumar, Satheesh Lama, Suraj Lin, Fan-Li Liu, Guei-Sheung Front Cell Dev Biol Cell and Developmental Biology Specific changes in the genome have been accomplished by the revolutionary gene-editing tool known as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. The advent of programmable RNA editing CRISPR/Cas nucleases has made this gene-editing tool safer and more precise. Specifically, CasRx, a family member of the Cas13d family, has shown great therapeutic potential. Here, we describe the in vitro methods of utilizing this powerful RNA editing platform and determine the RNA editing efficiencies for CasRx with different forms of guide RNAs (also known as gRNA or sgRNA). Frontiers Media S.A. 2021-06-11 /pmc/articles/PMC8226256/ /pubmed/34178991 http://dx.doi.org/10.3389/fcell.2021.667879 Text en Copyright © 2021 Chuang, Wang, Kumar, Lama, Lin and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Chuang, Yu-Fan
Wang, Peng-Yuan
Kumar, Satheesh
Lama, Suraj
Lin, Fan-Li
Liu, Guei-Sheung
Methods for in vitro CRISPR/CasRx-Mediated RNA Editing
title Methods for in vitro CRISPR/CasRx-Mediated RNA Editing
title_full Methods for in vitro CRISPR/CasRx-Mediated RNA Editing
title_fullStr Methods for in vitro CRISPR/CasRx-Mediated RNA Editing
title_full_unstemmed Methods for in vitro CRISPR/CasRx-Mediated RNA Editing
title_short Methods for in vitro CRISPR/CasRx-Mediated RNA Editing
title_sort methods for in vitro crispr/casrx-mediated rna editing
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226256/
https://www.ncbi.nlm.nih.gov/pubmed/34178991
http://dx.doi.org/10.3389/fcell.2021.667879
work_keys_str_mv AT chuangyufan methodsforinvitrocrisprcasrxmediatedrnaediting
AT wangpengyuan methodsforinvitrocrisprcasrxmediatedrnaediting
AT kumarsatheesh methodsforinvitrocrisprcasrxmediatedrnaediting
AT lamasuraj methodsforinvitrocrisprcasrxmediatedrnaediting
AT linfanli methodsforinvitrocrisprcasrxmediatedrnaediting
AT liugueisheung methodsforinvitrocrisprcasrxmediatedrnaediting

Ejemplares similares