Cargando…

Resistance to serine in Bacillus subtilis: identification of the serine transporter YbeC and of a metabolic network that links serine and threonine metabolism

The Gram‐positive bacterium Bacillus subtilis uses serine not only as a building block for proteins but also as an important precursor in many anabolic reactions. Moreover, a lack of serine results in the initiation of biofilm formation. However, excess serine inhibits the growth of B. subtilis. To...

Descripción completa

Detalles Bibliográficos
Autores principales: Klewing, Anika, Koo, Byoung‐Mo, Krüger, Larissa, Poehlein, Anja, Reuß, Daniel, Daniel, Rolf, Gross, Carol A., Stülke, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226366/
https://www.ncbi.nlm.nih.gov/pubmed/32743959
http://dx.doi.org/10.1111/1462-2920.15179
_version_ 1783712273655660544
author Klewing, Anika
Koo, Byoung‐Mo
Krüger, Larissa
Poehlein, Anja
Reuß, Daniel
Daniel, Rolf
Gross, Carol A.
Stülke, Jörg
author_facet Klewing, Anika
Koo, Byoung‐Mo
Krüger, Larissa
Poehlein, Anja
Reuß, Daniel
Daniel, Rolf
Gross, Carol A.
Stülke, Jörg
author_sort Klewing, Anika
collection PubMed
description The Gram‐positive bacterium Bacillus subtilis uses serine not only as a building block for proteins but also as an important precursor in many anabolic reactions. Moreover, a lack of serine results in the initiation of biofilm formation. However, excess serine inhibits the growth of B. subtilis. To unravel the underlying mechanisms, we isolated suppressor mutants that can tolerate toxic serine concentrations by three targeted and non‐targeted genome‐wide screens. All screens as well as genetic complementation in Escherichia coli identified the so far uncharacterized permease YbeC as the major serine transporter of B. subtilis. In addition to YbeC, the threonine transporters BcaP and YbxG make minor contributions to serine uptake. A strain lacking these three transporters was able to tolerate 100 mM serine whereas the wild type strain was already inhibited by 1 mM of the amino acid. The screen for serine‐resistant mutants also identified mutations that result in increased serine degradation and in increased expression of threonine biosynthetic enzymes suggesting that serine toxicity results from interference with threonine biosynthesis.
format Online
Article
Text
id pubmed-8226366
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley & Sons, Inc.
record_format MEDLINE/PubMed
spelling pubmed-82263662021-06-29 Resistance to serine in Bacillus subtilis: identification of the serine transporter YbeC and of a metabolic network that links serine and threonine metabolism Klewing, Anika Koo, Byoung‐Mo Krüger, Larissa Poehlein, Anja Reuß, Daniel Daniel, Rolf Gross, Carol A. Stülke, Jörg Environ Microbiol Research Articles The Gram‐positive bacterium Bacillus subtilis uses serine not only as a building block for proteins but also as an important precursor in many anabolic reactions. Moreover, a lack of serine results in the initiation of biofilm formation. However, excess serine inhibits the growth of B. subtilis. To unravel the underlying mechanisms, we isolated suppressor mutants that can tolerate toxic serine concentrations by three targeted and non‐targeted genome‐wide screens. All screens as well as genetic complementation in Escherichia coli identified the so far uncharacterized permease YbeC as the major serine transporter of B. subtilis. In addition to YbeC, the threonine transporters BcaP and YbxG make minor contributions to serine uptake. A strain lacking these three transporters was able to tolerate 100 mM serine whereas the wild type strain was already inhibited by 1 mM of the amino acid. The screen for serine‐resistant mutants also identified mutations that result in increased serine degradation and in increased expression of threonine biosynthetic enzymes suggesting that serine toxicity results from interference with threonine biosynthesis. John Wiley & Sons, Inc. 2020-08-13 2020-09 /pmc/articles/PMC8226366/ /pubmed/32743959 http://dx.doi.org/10.1111/1462-2920.15179 Text en © 2020 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Klewing, Anika
Koo, Byoung‐Mo
Krüger, Larissa
Poehlein, Anja
Reuß, Daniel
Daniel, Rolf
Gross, Carol A.
Stülke, Jörg
Resistance to serine in Bacillus subtilis: identification of the serine transporter YbeC and of a metabolic network that links serine and threonine metabolism
title Resistance to serine in Bacillus subtilis: identification of the serine transporter YbeC and of a metabolic network that links serine and threonine metabolism
title_full Resistance to serine in Bacillus subtilis: identification of the serine transporter YbeC and of a metabolic network that links serine and threonine metabolism
title_fullStr Resistance to serine in Bacillus subtilis: identification of the serine transporter YbeC and of a metabolic network that links serine and threonine metabolism
title_full_unstemmed Resistance to serine in Bacillus subtilis: identification of the serine transporter YbeC and of a metabolic network that links serine and threonine metabolism
title_short Resistance to serine in Bacillus subtilis: identification of the serine transporter YbeC and of a metabolic network that links serine and threonine metabolism
title_sort resistance to serine in bacillus subtilis: identification of the serine transporter ybec and of a metabolic network that links serine and threonine metabolism
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226366/
https://www.ncbi.nlm.nih.gov/pubmed/32743959
http://dx.doi.org/10.1111/1462-2920.15179
work_keys_str_mv AT klewinganika resistancetoserineinbacillussubtilisidentificationoftheserinetransporterybecandofametabolicnetworkthatlinksserineandthreoninemetabolism
AT koobyoungmo resistancetoserineinbacillussubtilisidentificationoftheserinetransporterybecandofametabolicnetworkthatlinksserineandthreoninemetabolism
AT krugerlarissa resistancetoserineinbacillussubtilisidentificationoftheserinetransporterybecandofametabolicnetworkthatlinksserineandthreoninemetabolism
AT poehleinanja resistancetoserineinbacillussubtilisidentificationoftheserinetransporterybecandofametabolicnetworkthatlinksserineandthreoninemetabolism
AT reußdaniel resistancetoserineinbacillussubtilisidentificationoftheserinetransporterybecandofametabolicnetworkthatlinksserineandthreoninemetabolism
AT danielrolf resistancetoserineinbacillussubtilisidentificationoftheserinetransporterybecandofametabolicnetworkthatlinksserineandthreoninemetabolism
AT grosscarola resistancetoserineinbacillussubtilisidentificationoftheserinetransporterybecandofametabolicnetworkthatlinksserineandthreoninemetabolism
AT stulkejorg resistancetoserineinbacillussubtilisidentificationoftheserinetransporterybecandofametabolicnetworkthatlinksserineandthreoninemetabolism