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Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer

SIMPLE SUMMARY: Standard treatment of locally advanced rectal cancer (LARC) consists of chemotherapy, radiotherapy, and surgery. Identification of radio-resistant (RR) and radio-sensitive (RS) LARC has been a major hurdle for patient-specific treatment. The development of biomarkers that can discrim...

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Autores principales: Lee, Jeeyong, Kwon, Junhye, Kim, DaYeon, Park, Misun, Kim, KwangSeok, Bae, InHwa, Kim, Hyunkyung, Kong, JoonSeog, Kim, Younjoo, Shin, UiSup, Kim, EunJu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226560/
https://www.ncbi.nlm.nih.gov/pubmed/34205090
http://dx.doi.org/10.3390/biology10060500
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author Lee, Jeeyong
Kwon, Junhye
Kim, DaYeon
Park, Misun
Kim, KwangSeok
Bae, InHwa
Kim, Hyunkyung
Kong, JoonSeog
Kim, Younjoo
Shin, UiSup
Kim, EunJu
author_facet Lee, Jeeyong
Kwon, Junhye
Kim, DaYeon
Park, Misun
Kim, KwangSeok
Bae, InHwa
Kim, Hyunkyung
Kong, JoonSeog
Kim, Younjoo
Shin, UiSup
Kim, EunJu
author_sort Lee, Jeeyong
collection PubMed
description SIMPLE SUMMARY: Standard treatment of locally advanced rectal cancer (LARC) consists of chemotherapy, radiotherapy, and surgery. Identification of radio-resistant (RR) and radio-sensitive (RS) LARC has been a major hurdle for patient-specific treatment. The development of biomarkers that can discriminate radio-responsiveness before surgery could improve standard treatment and minimize unwanted side effects. ABSTRACT: LARC patients were sorted according to their radio-responsiveness and patient-derived organoids were established from the respective cancer tissues. Expression profiles for each group were obtained using RNA-seq. Biological and bioinformatic analysis approaches were used in deciphering genes and pathways that participate in the radio-resistance of LARC. Thirty candidate genes encoding proteins involved in radio-responsiveness–related pathways, including the immune system, DNA repair and cell-cycle control, were identified. Interestingly, one of the candidate genes, cathepsin E (CTSE), exhibited differential methylation at the promoter region that was inversely correlated with the radio-resistance of patient-derived organoids, suggesting that methylation status could contribute to radio-responsiveness. On the basis of these results, we plan to pursue development of a gene chip for diagnosing the radio-responsiveness of LARC patients, with the hope that our efforts will ultimately improve the prognosis of LARC patients.
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spelling pubmed-82265602021-06-26 Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer Lee, Jeeyong Kwon, Junhye Kim, DaYeon Park, Misun Kim, KwangSeok Bae, InHwa Kim, Hyunkyung Kong, JoonSeog Kim, Younjoo Shin, UiSup Kim, EunJu Biology (Basel) Article SIMPLE SUMMARY: Standard treatment of locally advanced rectal cancer (LARC) consists of chemotherapy, radiotherapy, and surgery. Identification of radio-resistant (RR) and radio-sensitive (RS) LARC has been a major hurdle for patient-specific treatment. The development of biomarkers that can discriminate radio-responsiveness before surgery could improve standard treatment and minimize unwanted side effects. ABSTRACT: LARC patients were sorted according to their radio-responsiveness and patient-derived organoids were established from the respective cancer tissues. Expression profiles for each group were obtained using RNA-seq. Biological and bioinformatic analysis approaches were used in deciphering genes and pathways that participate in the radio-resistance of LARC. Thirty candidate genes encoding proteins involved in radio-responsiveness–related pathways, including the immune system, DNA repair and cell-cycle control, were identified. Interestingly, one of the candidate genes, cathepsin E (CTSE), exhibited differential methylation at the promoter region that was inversely correlated with the radio-resistance of patient-derived organoids, suggesting that methylation status could contribute to radio-responsiveness. On the basis of these results, we plan to pursue development of a gene chip for diagnosing the radio-responsiveness of LARC patients, with the hope that our efforts will ultimately improve the prognosis of LARC patients. MDPI 2021-06-03 /pmc/articles/PMC8226560/ /pubmed/34205090 http://dx.doi.org/10.3390/biology10060500 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Jeeyong
Kwon, Junhye
Kim, DaYeon
Park, Misun
Kim, KwangSeok
Bae, InHwa
Kim, Hyunkyung
Kong, JoonSeog
Kim, Younjoo
Shin, UiSup
Kim, EunJu
Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer
title Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer
title_full Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer
title_fullStr Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer
title_full_unstemmed Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer
title_short Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer
title_sort gene expression profiles associated with radio-responsiveness in locally advanced rectal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226560/
https://www.ncbi.nlm.nih.gov/pubmed/34205090
http://dx.doi.org/10.3390/biology10060500
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