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Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer
SIMPLE SUMMARY: Standard treatment of locally advanced rectal cancer (LARC) consists of chemotherapy, radiotherapy, and surgery. Identification of radio-resistant (RR) and radio-sensitive (RS) LARC has been a major hurdle for patient-specific treatment. The development of biomarkers that can discrim...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226560/ https://www.ncbi.nlm.nih.gov/pubmed/34205090 http://dx.doi.org/10.3390/biology10060500 |
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author | Lee, Jeeyong Kwon, Junhye Kim, DaYeon Park, Misun Kim, KwangSeok Bae, InHwa Kim, Hyunkyung Kong, JoonSeog Kim, Younjoo Shin, UiSup Kim, EunJu |
author_facet | Lee, Jeeyong Kwon, Junhye Kim, DaYeon Park, Misun Kim, KwangSeok Bae, InHwa Kim, Hyunkyung Kong, JoonSeog Kim, Younjoo Shin, UiSup Kim, EunJu |
author_sort | Lee, Jeeyong |
collection | PubMed |
description | SIMPLE SUMMARY: Standard treatment of locally advanced rectal cancer (LARC) consists of chemotherapy, radiotherapy, and surgery. Identification of radio-resistant (RR) and radio-sensitive (RS) LARC has been a major hurdle for patient-specific treatment. The development of biomarkers that can discriminate radio-responsiveness before surgery could improve standard treatment and minimize unwanted side effects. ABSTRACT: LARC patients were sorted according to their radio-responsiveness and patient-derived organoids were established from the respective cancer tissues. Expression profiles for each group were obtained using RNA-seq. Biological and bioinformatic analysis approaches were used in deciphering genes and pathways that participate in the radio-resistance of LARC. Thirty candidate genes encoding proteins involved in radio-responsiveness–related pathways, including the immune system, DNA repair and cell-cycle control, were identified. Interestingly, one of the candidate genes, cathepsin E (CTSE), exhibited differential methylation at the promoter region that was inversely correlated with the radio-resistance of patient-derived organoids, suggesting that methylation status could contribute to radio-responsiveness. On the basis of these results, we plan to pursue development of a gene chip for diagnosing the radio-responsiveness of LARC patients, with the hope that our efforts will ultimately improve the prognosis of LARC patients. |
format | Online Article Text |
id | pubmed-8226560 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82265602021-06-26 Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer Lee, Jeeyong Kwon, Junhye Kim, DaYeon Park, Misun Kim, KwangSeok Bae, InHwa Kim, Hyunkyung Kong, JoonSeog Kim, Younjoo Shin, UiSup Kim, EunJu Biology (Basel) Article SIMPLE SUMMARY: Standard treatment of locally advanced rectal cancer (LARC) consists of chemotherapy, radiotherapy, and surgery. Identification of radio-resistant (RR) and radio-sensitive (RS) LARC has been a major hurdle for patient-specific treatment. The development of biomarkers that can discriminate radio-responsiveness before surgery could improve standard treatment and minimize unwanted side effects. ABSTRACT: LARC patients were sorted according to their radio-responsiveness and patient-derived organoids were established from the respective cancer tissues. Expression profiles for each group were obtained using RNA-seq. Biological and bioinformatic analysis approaches were used in deciphering genes and pathways that participate in the radio-resistance of LARC. Thirty candidate genes encoding proteins involved in radio-responsiveness–related pathways, including the immune system, DNA repair and cell-cycle control, were identified. Interestingly, one of the candidate genes, cathepsin E (CTSE), exhibited differential methylation at the promoter region that was inversely correlated with the radio-resistance of patient-derived organoids, suggesting that methylation status could contribute to radio-responsiveness. On the basis of these results, we plan to pursue development of a gene chip for diagnosing the radio-responsiveness of LARC patients, with the hope that our efforts will ultimately improve the prognosis of LARC patients. MDPI 2021-06-03 /pmc/articles/PMC8226560/ /pubmed/34205090 http://dx.doi.org/10.3390/biology10060500 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Jeeyong Kwon, Junhye Kim, DaYeon Park, Misun Kim, KwangSeok Bae, InHwa Kim, Hyunkyung Kong, JoonSeog Kim, Younjoo Shin, UiSup Kim, EunJu Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer |
title | Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer |
title_full | Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer |
title_fullStr | Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer |
title_full_unstemmed | Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer |
title_short | Gene Expression Profiles Associated with Radio-Responsiveness in Locally Advanced Rectal Cancer |
title_sort | gene expression profiles associated with radio-responsiveness in locally advanced rectal cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226560/ https://www.ncbi.nlm.nih.gov/pubmed/34205090 http://dx.doi.org/10.3390/biology10060500 |
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