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Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications

A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnosti...

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Detalles Bibliográficos
Autores principales: Wan, Nan, Jiang, Yu, Huang, Jiamei, Oueslati, Rania, Eda, Shigetoshi, Wu, Jayne, Lin, Xiaogang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226656/
https://www.ncbi.nlm.nih.gov/pubmed/34207808
http://dx.doi.org/10.3390/s21123985
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author Wan, Nan
Jiang, Yu
Huang, Jiamei
Oueslati, Rania
Eda, Shigetoshi
Wu, Jayne
Lin, Xiaogang
author_facet Wan, Nan
Jiang, Yu
Huang, Jiamei
Oueslati, Rania
Eda, Shigetoshi
Wu, Jayne
Lin, Xiaogang
author_sort Wan, Nan
collection PubMed
description A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnostics. Presently, most miRNA detection methods suffer from drawbacks, e.g., low sensitivity, long assay time, expensive equipment, trained personnel, or unsuitability for point-of-care. New methodologies are needed to overcome these limitations to allow rapid, sensitive, low-cost, easy-to-use, and portable methods for miRNA detection at the point of care. In this work, to overcome these shortcomings, we integrated capacitive sensing and alternating current electrokinetic effects to detect specific miRNA-16b molecules, as a model, with the limit of detection reaching 1.0 femto molar (fM) levels. The specificity of the sensor was verified by testing miRNA-25, which has the same length as miRNA-16b. The sensor we developed demonstrated significant improvements in sensitivity, response time and cost over other miRNA detection methods, and has application potential at point-of-care.
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spelling pubmed-82266562021-06-26 Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications Wan, Nan Jiang, Yu Huang, Jiamei Oueslati, Rania Eda, Shigetoshi Wu, Jayne Lin, Xiaogang Sensors (Basel) Article A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnostics. Presently, most miRNA detection methods suffer from drawbacks, e.g., low sensitivity, long assay time, expensive equipment, trained personnel, or unsuitability for point-of-care. New methodologies are needed to overcome these limitations to allow rapid, sensitive, low-cost, easy-to-use, and portable methods for miRNA detection at the point of care. In this work, to overcome these shortcomings, we integrated capacitive sensing and alternating current electrokinetic effects to detect specific miRNA-16b molecules, as a model, with the limit of detection reaching 1.0 femto molar (fM) levels. The specificity of the sensor was verified by testing miRNA-25, which has the same length as miRNA-16b. The sensor we developed demonstrated significant improvements in sensitivity, response time and cost over other miRNA detection methods, and has application potential at point-of-care. MDPI 2021-06-09 /pmc/articles/PMC8226656/ /pubmed/34207808 http://dx.doi.org/10.3390/s21123985 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wan, Nan
Jiang, Yu
Huang, Jiamei
Oueslati, Rania
Eda, Shigetoshi
Wu, Jayne
Lin, Xiaogang
Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications
title Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications
title_full Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications
title_fullStr Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications
title_full_unstemmed Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications
title_short Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications
title_sort rapid and sensitive detection of mirna based on ac electrokinetic capacitive sensing for point-of-care applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226656/
https://www.ncbi.nlm.nih.gov/pubmed/34207808
http://dx.doi.org/10.3390/s21123985
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