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Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum
In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226682/ https://www.ncbi.nlm.nih.gov/pubmed/34200643 http://dx.doi.org/10.3390/bios11060191 |
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author | Kim, Hyun Tae Jin, Enjian Lee, Min-Ho |
author_facet | Kim, Hyun Tae Jin, Enjian Lee, Min-Ho |
author_sort | Kim, Hyun Tae |
collection | PubMed |
description | In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R(2) = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R(2) = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ. |
format | Online Article Text |
id | pubmed-8226682 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82266822021-06-26 Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum Kim, Hyun Tae Jin, Enjian Lee, Min-Ho Biosensors (Basel) Article In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R(2) = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R(2) = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ. MDPI 2021-06-10 /pmc/articles/PMC8226682/ /pubmed/34200643 http://dx.doi.org/10.3390/bios11060191 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Hyun Tae Jin, Enjian Lee, Min-Ho Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum |
title | Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum |
title_full | Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum |
title_fullStr | Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum |
title_full_unstemmed | Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum |
title_short | Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum |
title_sort | portable chemiluminescence-based lateral flow assay platform for the detection of cortisol in human serum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226682/ https://www.ncbi.nlm.nih.gov/pubmed/34200643 http://dx.doi.org/10.3390/bios11060191 |
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