SCA Medium: A New Culture Medium for the Isolation of All Candida auris Clades

Candida auris is an emerging multidrug-resistant yeast causing nosocomial infections and associated with high mortality in immunocompromised patients. Rapid identification and characterisation are necessary for diagnosis and containing its spread. In this study, we present a selective culture medium...

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Autores principales: Ibrahim, Ahmad, Peyclit, Lucie, Abdallah, Rim, Khelaifia, Saber, Chamieh, Amanda, Rolain, Jean-Marc, Bittar, Fadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226795/
https://www.ncbi.nlm.nih.gov/pubmed/34072592
http://dx.doi.org/10.3390/jof7060433
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author Ibrahim, Ahmad
Peyclit, Lucie
Abdallah, Rim
Khelaifia, Saber
Chamieh, Amanda
Rolain, Jean-Marc
Bittar, Fadi
author_facet Ibrahim, Ahmad
Peyclit, Lucie
Abdallah, Rim
Khelaifia, Saber
Chamieh, Amanda
Rolain, Jean-Marc
Bittar, Fadi
author_sort Ibrahim, Ahmad
collection PubMed
description Candida auris is an emerging multidrug-resistant yeast causing nosocomial infections and associated with high mortality in immunocompromised patients. Rapid identification and characterisation are necessary for diagnosis and containing its spread. In this study, we present a selective culture medium for all C. auris clades. This medium is sensitive with a limit of detection ranging between 10(1) and 10(2) CFU/mL. The 100% specificity of SCA (specific C. auris) medium is confirmed on a set of 135 Candida strains, 50 bacterial species and 200 human stool samples. Thus, this medium specifically selects for C. auris isolation from clinical samples, allowing the latter to study its phenotypic profile.
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spelling pubmed-82267952021-06-26 SCA Medium: A New Culture Medium for the Isolation of All Candida auris Clades Ibrahim, Ahmad Peyclit, Lucie Abdallah, Rim Khelaifia, Saber Chamieh, Amanda Rolain, Jean-Marc Bittar, Fadi J Fungi (Basel) Brief Report Candida auris is an emerging multidrug-resistant yeast causing nosocomial infections and associated with high mortality in immunocompromised patients. Rapid identification and characterisation are necessary for diagnosis and containing its spread. In this study, we present a selective culture medium for all C. auris clades. This medium is sensitive with a limit of detection ranging between 10(1) and 10(2) CFU/mL. The 100% specificity of SCA (specific C. auris) medium is confirmed on a set of 135 Candida strains, 50 bacterial species and 200 human stool samples. Thus, this medium specifically selects for C. auris isolation from clinical samples, allowing the latter to study its phenotypic profile. MDPI 2021-05-29 /pmc/articles/PMC8226795/ /pubmed/34072592 http://dx.doi.org/10.3390/jof7060433 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Ibrahim, Ahmad
Peyclit, Lucie
Abdallah, Rim
Khelaifia, Saber
Chamieh, Amanda
Rolain, Jean-Marc
Bittar, Fadi
SCA Medium: A New Culture Medium for the Isolation of All Candida auris Clades
title SCA Medium: A New Culture Medium for the Isolation of All Candida auris Clades
title_full SCA Medium: A New Culture Medium for the Isolation of All Candida auris Clades
title_fullStr SCA Medium: A New Culture Medium for the Isolation of All Candida auris Clades
title_full_unstemmed SCA Medium: A New Culture Medium for the Isolation of All Candida auris Clades
title_short SCA Medium: A New Culture Medium for the Isolation of All Candida auris Clades
title_sort sca medium: a new culture medium for the isolation of all candida auris clades
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226795/
https://www.ncbi.nlm.nih.gov/pubmed/34072592
http://dx.doi.org/10.3390/jof7060433
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