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Identification of Volatile Sulfur Compounds Produced by Schizophyllum commune
Schizophyllum commune is a causative agent of allergic bronchopulmonary mycosis, allergic fungal rhinosinusitis, and basidiomycosis. Diagnosis of these diseases remains difficult because no commercially available tool exists to identify the pathogen. Unique volatile organic compounds produced by a p...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226890/ https://www.ncbi.nlm.nih.gov/pubmed/34201392 http://dx.doi.org/10.3390/jof7060465 |
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author | Toyotome, Takahito Takino, Masahiko Takaya, Masahiro Yahiro, Maki Kamei, Katsuhiko |
author_facet | Toyotome, Takahito Takino, Masahiko Takaya, Masahiro Yahiro, Maki Kamei, Katsuhiko |
author_sort | Toyotome, Takahito |
collection | PubMed |
description | Schizophyllum commune is a causative agent of allergic bronchopulmonary mycosis, allergic fungal rhinosinusitis, and basidiomycosis. Diagnosis of these diseases remains difficult because no commercially available tool exists to identify the pathogen. Unique volatile organic compounds produced by a pathogen might be useful for non-invasive diagnosis. Here, we explored microbial volatile organic compounds produced by S. commune. Volatile sulfur compounds, dimethyl disulfide (48 of 49 strains) and methyl ethyl disulfide (49 of 49 strains), diethyl disulfide (34 of 49 strains), dimethyl trisulfide (40 of 49 strains), and dimethyl tetrasulfide (32 of 49 strains) were detected from headspace air in S. commune cultured vials. Every S. commune strain produced at least one volatile sulfur compound analyzed in this study. Those volatile sulfur compounds were not detected from the cultures of Aspergillus spp. (A. fumigatus, A. flavus, A. niger, and A. terreus), which are other major causative agents of allergic bronchopulmonary mycosis. The last, we examined H(2)S detection using lead acetate paper. Headspace air from S. commune rapidly turned the lead acetate paper black. These results suggest that those volatile sulfur compounds are potent targets for the diagnosis of S. commune and infectious diseases. |
format | Online Article Text |
id | pubmed-8226890 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82268902021-06-26 Identification of Volatile Sulfur Compounds Produced by Schizophyllum commune Toyotome, Takahito Takino, Masahiko Takaya, Masahiro Yahiro, Maki Kamei, Katsuhiko J Fungi (Basel) Article Schizophyllum commune is a causative agent of allergic bronchopulmonary mycosis, allergic fungal rhinosinusitis, and basidiomycosis. Diagnosis of these diseases remains difficult because no commercially available tool exists to identify the pathogen. Unique volatile organic compounds produced by a pathogen might be useful for non-invasive diagnosis. Here, we explored microbial volatile organic compounds produced by S. commune. Volatile sulfur compounds, dimethyl disulfide (48 of 49 strains) and methyl ethyl disulfide (49 of 49 strains), diethyl disulfide (34 of 49 strains), dimethyl trisulfide (40 of 49 strains), and dimethyl tetrasulfide (32 of 49 strains) were detected from headspace air in S. commune cultured vials. Every S. commune strain produced at least one volatile sulfur compound analyzed in this study. Those volatile sulfur compounds were not detected from the cultures of Aspergillus spp. (A. fumigatus, A. flavus, A. niger, and A. terreus), which are other major causative agents of allergic bronchopulmonary mycosis. The last, we examined H(2)S detection using lead acetate paper. Headspace air from S. commune rapidly turned the lead acetate paper black. These results suggest that those volatile sulfur compounds are potent targets for the diagnosis of S. commune and infectious diseases. MDPI 2021-06-08 /pmc/articles/PMC8226890/ /pubmed/34201392 http://dx.doi.org/10.3390/jof7060465 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Toyotome, Takahito Takino, Masahiko Takaya, Masahiro Yahiro, Maki Kamei, Katsuhiko Identification of Volatile Sulfur Compounds Produced by Schizophyllum commune |
title | Identification of Volatile Sulfur Compounds Produced by Schizophyllum commune |
title_full | Identification of Volatile Sulfur Compounds Produced by Schizophyllum commune |
title_fullStr | Identification of Volatile Sulfur Compounds Produced by Schizophyllum commune |
title_full_unstemmed | Identification of Volatile Sulfur Compounds Produced by Schizophyllum commune |
title_short | Identification of Volatile Sulfur Compounds Produced by Schizophyllum commune |
title_sort | identification of volatile sulfur compounds produced by schizophyllum commune |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226890/ https://www.ncbi.nlm.nih.gov/pubmed/34201392 http://dx.doi.org/10.3390/jof7060465 |
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