Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel

Bovine coronavirus (BCoV) is the causative agent of winter dysentery (WD). In adult dairy cattle, WD is characterized by hemorrhagic diarrhea and a reduction in milk production. Therefore, WD leads to significant economic losses in dairy farms. In this study, we aimed to isolate and characterize loc...

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Detalles Bibliográficos
Autores principales: David, Dan, Storm, Nick, Ilan, Waksman, Sol, Asaf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226893/
https://www.ncbi.nlm.nih.gov/pubmed/34199933
http://dx.doi.org/10.3390/v13061070
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author David, Dan
Storm, Nick
Ilan, Waksman
Sol, Asaf
author_facet David, Dan
Storm, Nick
Ilan, Waksman
Sol, Asaf
author_sort David, Dan
collection PubMed
description Bovine coronavirus (BCoV) is the causative agent of winter dysentery (WD). In adult dairy cattle, WD is characterized by hemorrhagic diarrhea and a reduction in milk production. Therefore, WD leads to significant economic losses in dairy farms. In this study, we aimed to isolate and characterize local BCoV strains. BCoV positive samples, collected during 2017–2021, were used to amplify and sequence the S1 domain of S glycoprotein and the full hemagglutinin esterase gene. Based on our molecular analysis, local strains belong to different genetic variants circulating in dairy farms in Israel. Phylogenetic analysis revealed that all local strains clustered together and in proximity to other BCoV circulating in the area. Additionally, we found that local strains are genetically distant from the reference enteric strain Mebus. To our knowledge, this is the first report providing molecular data on BCoV circulating in Israel.
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spelling pubmed-82268932021-06-26 Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel David, Dan Storm, Nick Ilan, Waksman Sol, Asaf Viruses Brief Report Bovine coronavirus (BCoV) is the causative agent of winter dysentery (WD). In adult dairy cattle, WD is characterized by hemorrhagic diarrhea and a reduction in milk production. Therefore, WD leads to significant economic losses in dairy farms. In this study, we aimed to isolate and characterize local BCoV strains. BCoV positive samples, collected during 2017–2021, were used to amplify and sequence the S1 domain of S glycoprotein and the full hemagglutinin esterase gene. Based on our molecular analysis, local strains belong to different genetic variants circulating in dairy farms in Israel. Phylogenetic analysis revealed that all local strains clustered together and in proximity to other BCoV circulating in the area. Additionally, we found that local strains are genetically distant from the reference enteric strain Mebus. To our knowledge, this is the first report providing molecular data on BCoV circulating in Israel. MDPI 2021-06-04 /pmc/articles/PMC8226893/ /pubmed/34199933 http://dx.doi.org/10.3390/v13061070 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
David, Dan
Storm, Nick
Ilan, Waksman
Sol, Asaf
Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel
title Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel
title_full Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel
title_fullStr Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel
title_full_unstemmed Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel
title_short Characterization of Winter Dysentery Bovine Coronavirus Isolated from Cattle in Israel
title_sort characterization of winter dysentery bovine coronavirus isolated from cattle in israel
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226893/
https://www.ncbi.nlm.nih.gov/pubmed/34199933
http://dx.doi.org/10.3390/v13061070
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