Cargando…

Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa

Multidrug-resistant Pseudomonas aeruginosa poses a serious problem due to hospital- and healthcare-associated infections. A major drug resistance mechanism of P. aeruginosa involves active efflux via resistance nodulation cell division (RND)-type multidrug efflux pumps of which MexXY is increasingly...

Descripción completa

Detalles Bibliográficos
Autores principales: Ikarashi, Kei, Kutsuna, Ryo, Tomida, Junko, Kawamura, Yoshiaki, Morita, Yuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226967/
https://www.ncbi.nlm.nih.gov/pubmed/34073068
http://dx.doi.org/10.3390/antibiotics10060658
_version_ 1783712413351149568
author Ikarashi, Kei
Kutsuna, Ryo
Tomida, Junko
Kawamura, Yoshiaki
Morita, Yuji
author_facet Ikarashi, Kei
Kutsuna, Ryo
Tomida, Junko
Kawamura, Yoshiaki
Morita, Yuji
author_sort Ikarashi, Kei
collection PubMed
description Multidrug-resistant Pseudomonas aeruginosa poses a serious problem due to hospital- and healthcare-associated infections. A major drug resistance mechanism of P. aeruginosa involves active efflux via resistance nodulation cell division (RND)-type multidrug efflux pumps of which MexXY is increasingly recognized as a primary determinant of aminoglycoside resistance in P. aeruginosa. MexXY overexpression is often observed in drug-resistant P. aeruginosa clinical isolates. MexXY deficiency increased pyoverdine production in all four P. aeruginosa strains we tested. MexXY-overproducing multidrug-resistant P. aeruginosa PA7 exhibited the greatest effect among the strains. Complementation with a MexXY-expressing plasmid restored low-level pyoverdine production in a MexXY-deficient P. aeruginosa mutant from PA7, indicating that MexXY expression decreases pyoverdine production. Because P. aeruginosa produces pyoverdine to acquire iron, MexXY-deficient mutants might be more susceptible to iron deficiency than MexXY-producing strains or might require extra iron. High-risk clones of multidrug-resistant P. aeruginosa reportedly tend to be MexXY overproducers but defective pyoverdine producers. This study suggests that P. aeruginosa reduces production of a virulence factor after acquiring a drug resistance factor.
format Online
Article
Text
id pubmed-8226967
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-82269672021-06-26 Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa Ikarashi, Kei Kutsuna, Ryo Tomida, Junko Kawamura, Yoshiaki Morita, Yuji Antibiotics (Basel) Communication Multidrug-resistant Pseudomonas aeruginosa poses a serious problem due to hospital- and healthcare-associated infections. A major drug resistance mechanism of P. aeruginosa involves active efflux via resistance nodulation cell division (RND)-type multidrug efflux pumps of which MexXY is increasingly recognized as a primary determinant of aminoglycoside resistance in P. aeruginosa. MexXY overexpression is often observed in drug-resistant P. aeruginosa clinical isolates. MexXY deficiency increased pyoverdine production in all four P. aeruginosa strains we tested. MexXY-overproducing multidrug-resistant P. aeruginosa PA7 exhibited the greatest effect among the strains. Complementation with a MexXY-expressing plasmid restored low-level pyoverdine production in a MexXY-deficient P. aeruginosa mutant from PA7, indicating that MexXY expression decreases pyoverdine production. Because P. aeruginosa produces pyoverdine to acquire iron, MexXY-deficient mutants might be more susceptible to iron deficiency than MexXY-producing strains or might require extra iron. High-risk clones of multidrug-resistant P. aeruginosa reportedly tend to be MexXY overproducers but defective pyoverdine producers. This study suggests that P. aeruginosa reduces production of a virulence factor after acquiring a drug resistance factor. MDPI 2021-05-31 /pmc/articles/PMC8226967/ /pubmed/34073068 http://dx.doi.org/10.3390/antibiotics10060658 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Ikarashi, Kei
Kutsuna, Ryo
Tomida, Junko
Kawamura, Yoshiaki
Morita, Yuji
Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa
title Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa
title_full Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa
title_fullStr Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa
title_full_unstemmed Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa
title_short Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa
title_sort overexpression of the mexxy multidrug efflux system correlates with deficient pyoverdine production in pseudomonas aeruginosa
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226967/
https://www.ncbi.nlm.nih.gov/pubmed/34073068
http://dx.doi.org/10.3390/antibiotics10060658
work_keys_str_mv AT ikarashikei overexpressionofthemexxymultidrugeffluxsystemcorrelateswithdeficientpyoverdineproductioninpseudomonasaeruginosa
AT kutsunaryo overexpressionofthemexxymultidrugeffluxsystemcorrelateswithdeficientpyoverdineproductioninpseudomonasaeruginosa
AT tomidajunko overexpressionofthemexxymultidrugeffluxsystemcorrelateswithdeficientpyoverdineproductioninpseudomonasaeruginosa
AT kawamurayoshiaki overexpressionofthemexxymultidrugeffluxsystemcorrelateswithdeficientpyoverdineproductioninpseudomonasaeruginosa
AT moritayuji overexpressionofthemexxymultidrugeffluxsystemcorrelateswithdeficientpyoverdineproductioninpseudomonasaeruginosa