Cargando…
Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa
Multidrug-resistant Pseudomonas aeruginosa poses a serious problem due to hospital- and healthcare-associated infections. A major drug resistance mechanism of P. aeruginosa involves active efflux via resistance nodulation cell division (RND)-type multidrug efflux pumps of which MexXY is increasingly...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226967/ https://www.ncbi.nlm.nih.gov/pubmed/34073068 http://dx.doi.org/10.3390/antibiotics10060658 |
_version_ | 1783712413351149568 |
---|---|
author | Ikarashi, Kei Kutsuna, Ryo Tomida, Junko Kawamura, Yoshiaki Morita, Yuji |
author_facet | Ikarashi, Kei Kutsuna, Ryo Tomida, Junko Kawamura, Yoshiaki Morita, Yuji |
author_sort | Ikarashi, Kei |
collection | PubMed |
description | Multidrug-resistant Pseudomonas aeruginosa poses a serious problem due to hospital- and healthcare-associated infections. A major drug resistance mechanism of P. aeruginosa involves active efflux via resistance nodulation cell division (RND)-type multidrug efflux pumps of which MexXY is increasingly recognized as a primary determinant of aminoglycoside resistance in P. aeruginosa. MexXY overexpression is often observed in drug-resistant P. aeruginosa clinical isolates. MexXY deficiency increased pyoverdine production in all four P. aeruginosa strains we tested. MexXY-overproducing multidrug-resistant P. aeruginosa PA7 exhibited the greatest effect among the strains. Complementation with a MexXY-expressing plasmid restored low-level pyoverdine production in a MexXY-deficient P. aeruginosa mutant from PA7, indicating that MexXY expression decreases pyoverdine production. Because P. aeruginosa produces pyoverdine to acquire iron, MexXY-deficient mutants might be more susceptible to iron deficiency than MexXY-producing strains or might require extra iron. High-risk clones of multidrug-resistant P. aeruginosa reportedly tend to be MexXY overproducers but defective pyoverdine producers. This study suggests that P. aeruginosa reduces production of a virulence factor after acquiring a drug resistance factor. |
format | Online Article Text |
id | pubmed-8226967 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82269672021-06-26 Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa Ikarashi, Kei Kutsuna, Ryo Tomida, Junko Kawamura, Yoshiaki Morita, Yuji Antibiotics (Basel) Communication Multidrug-resistant Pseudomonas aeruginosa poses a serious problem due to hospital- and healthcare-associated infections. A major drug resistance mechanism of P. aeruginosa involves active efflux via resistance nodulation cell division (RND)-type multidrug efflux pumps of which MexXY is increasingly recognized as a primary determinant of aminoglycoside resistance in P. aeruginosa. MexXY overexpression is often observed in drug-resistant P. aeruginosa clinical isolates. MexXY deficiency increased pyoverdine production in all four P. aeruginosa strains we tested. MexXY-overproducing multidrug-resistant P. aeruginosa PA7 exhibited the greatest effect among the strains. Complementation with a MexXY-expressing plasmid restored low-level pyoverdine production in a MexXY-deficient P. aeruginosa mutant from PA7, indicating that MexXY expression decreases pyoverdine production. Because P. aeruginosa produces pyoverdine to acquire iron, MexXY-deficient mutants might be more susceptible to iron deficiency than MexXY-producing strains or might require extra iron. High-risk clones of multidrug-resistant P. aeruginosa reportedly tend to be MexXY overproducers but defective pyoverdine producers. This study suggests that P. aeruginosa reduces production of a virulence factor after acquiring a drug resistance factor. MDPI 2021-05-31 /pmc/articles/PMC8226967/ /pubmed/34073068 http://dx.doi.org/10.3390/antibiotics10060658 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Ikarashi, Kei Kutsuna, Ryo Tomida, Junko Kawamura, Yoshiaki Morita, Yuji Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa |
title | Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa |
title_full | Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa |
title_fullStr | Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa |
title_full_unstemmed | Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa |
title_short | Overexpression of the MexXY Multidrug Efflux System Correlates with Deficient Pyoverdine Production in Pseudomonas aeruginosa |
title_sort | overexpression of the mexxy multidrug efflux system correlates with deficient pyoverdine production in pseudomonas aeruginosa |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226967/ https://www.ncbi.nlm.nih.gov/pubmed/34073068 http://dx.doi.org/10.3390/antibiotics10060658 |
work_keys_str_mv | AT ikarashikei overexpressionofthemexxymultidrugeffluxsystemcorrelateswithdeficientpyoverdineproductioninpseudomonasaeruginosa AT kutsunaryo overexpressionofthemexxymultidrugeffluxsystemcorrelateswithdeficientpyoverdineproductioninpseudomonasaeruginosa AT tomidajunko overexpressionofthemexxymultidrugeffluxsystemcorrelateswithdeficientpyoverdineproductioninpseudomonasaeruginosa AT kawamurayoshiaki overexpressionofthemexxymultidrugeffluxsystemcorrelateswithdeficientpyoverdineproductioninpseudomonasaeruginosa AT moritayuji overexpressionofthemexxymultidrugeffluxsystemcorrelateswithdeficientpyoverdineproductioninpseudomonasaeruginosa |