Characteristics of the Peritrophic Matrix of the Silkworm, Bombyx mori and Factors Influencing Its Formation

SIMPLE SUMMARY: The insect midgut is an important digestive organ with the peritrophic matrix (PM) being a semi-permeable membrane secreted by the midgut cells. The PM plays an important role in improving midgut digestion efficiency and protecting the midgut from food particles and exogenous pathogens. The silkworm, Bombyx mori, is an economically important insect. Understanding the structure of the PM is necessary for studying its function, but characteristics of PM in B. mori have been rarely reported. In this study, we conducted a comprehensive study on the PM structure of the PM in silkworms and found its thickness increased gradually during growth, but there was no difference in the thickness comparing the anterior, middle, and posterior regions. Permeability of the PM gradually decreased from the anterior to posterior regions. In addition, we found the formation of the PM was influenced by food ingestion and the gut microbiota. ABSTRACT: The peritrophic matrix (PM) secreted by the midgut cells of insects is formed by the binding of PM proteins to chitin fibrils. The PM envelops the food bolus, serving as a barrier between the content of the midgut lumen and its epithelium, and plays a protective role for epithelial cells against mechanical damage, pathogens, toxins, and other harmful substances. However, few studies have investigated the characteristics and synthesis factors of the PM in the silkworm, Bombyx mori. Here, we examined the characteristics of the PM in the silkworms. The PM thickness of the silkworms increased gradually during growth, while there was no significant difference in thickness along the entire PM region. Permeability of the PM decreased gradually from the anterior to posterior PM. We also found that PM synthesis was affected by food ingestion and the gut microbiota. Our results are beneficial for future studies regarding the function of the PM in silkworms.