Screening of Lactic Acid Bacteria with Inhibitory Activity against ETEC K88 as Feed Additive and the Effects on Sows and Piglets

SIMPLE SUMMARY: Numerous reports have suggested that lactic acid bacteria (LAB), which are important probiotics, can protect animals against pathogen-induced injury and inflammation, regulate gut microflora, enhance digestive tract function, improve animal growth performance, and decrease the incidence of diarrhea caused by enterotoxigenic (ETEC) that expresses K88. This research selected Lactobacillus (L.) reuteri P7, L. amylovorus P8, and L. johnsonii P15 with good inhibition against ETEC K88 and excellent probiotic properties screened from 295 LAB strains isolated from fecal samples from 55 healthy weaned piglets for a study on feeding of sows in late pregnancy and weaned piglets. Feed supplementation with these three strains improved reproductive performance of sows and growth performance of piglets, decreased the incidence of diarrhea in piglets, and increased the antioxidant capacity of serum in both sows and piglets. Therefore, L. reuteri P7, L. amylovorus P8, and L. johnsonii P15 might be considered as potential antibiotic alternatives for further study. ABSTRACT: Enterotoxigenic Escherichia coli (ETEC), which expresses K88 is the principal microorganism responsible for bacterial diarrhea in pig husbandry, and the indiscriminate use of antibiotics has caused many problems; therefore, antibiotics need to be replaced in order to prevent diarrhea caused by ETEC K88. The objective of this study was to screen excellent lactic acid bacteria (LAB) strains that inhibit ETEC K88 and explore their effects as probiotic supplementation on reproduction, growth performance, diarrheal incidence, and antioxidant capacity of serum in sows and weaned piglets. Three LAB strains, P7, P8, and P15, screened from 295 LAB strains and assigned to Lactobacillus (L.) reuteri, L. amylovorus, and L. johnsonii with high inhibitory activity against ETEC K88 were selected for a study on feeding of sows and weaned piglets. These strains were chosen for their good physiological and biochemical characteristics, excellent exopolysaccharide (EPS) production capacity, hydrophobicity, auto-aggregation ability, survival in gastrointestinal (GI) fluids, lack of hemolytic activity, and broad-spectrum activity against a wide range of microorganisms. The results indicate that LAB strains P7, P8, and P15 had significant effects on improving the reproductive performance of sows and the growth performance of weaned piglets, increasing the activity of antioxidant enzymes and immune indexes in both.