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Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus

An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV). However, the distinct antigenicity and immunogenicity of VP1, VP0, and VP3 of FMDV serotype SA...

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Autores principales: Li, Guoxiu, Wubshet, Ashenafi Kiros, Ding, Yaozhong, Li, Qian, Dai, Junfei, Wang, Yang, Hou, Qian, Chen, Jiao, Ma, Bing, Szczotka-Bochniarz, Anna, Szathmary, Susan, Zhang, Yongguang, Zhang, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227194/
https://www.ncbi.nlm.nih.gov/pubmed/34072100
http://dx.doi.org/10.3390/v13061005
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author Li, Guoxiu
Wubshet, Ashenafi Kiros
Ding, Yaozhong
Li, Qian
Dai, Junfei
Wang, Yang
Hou, Qian
Chen, Jiao
Ma, Bing
Szczotka-Bochniarz, Anna
Szathmary, Susan
Zhang, Yongguang
Zhang, Jie
author_facet Li, Guoxiu
Wubshet, Ashenafi Kiros
Ding, Yaozhong
Li, Qian
Dai, Junfei
Wang, Yang
Hou, Qian
Chen, Jiao
Ma, Bing
Szczotka-Bochniarz, Anna
Szathmary, Susan
Zhang, Yongguang
Zhang, Jie
author_sort Li, Guoxiu
collection PubMed
description An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV). However, the distinct antigenicity and immunogenicity of VP1, VP0, and VP3 of FMDV serotype SAT2 are poorly understood. Similarly, the particular roles of the three structural proteins in novel vaccine design and development remain unexplained. We therefore constructed VP1, VP0, and VP3 encoding gene (SAT2:JX014256 strain) separately fused with His-SUMO (histidine-small ubiquitin-related modifier) inserted into pET-32a cassette to express the three recombinant proteins and separately evaluated their antigenicity and immunogenicity in mice. The fusion protein was successfully expressed and purified by the Ni-NTA resin chromatography. The level of serum antibody, spleen lymphocyte proliferation, and cytokines against the three distinct recombinant proteins were analyzed. Results showed that the anti-FMDV humoral response was triggered by these proteins, and the fusion proteins did enhance the splenocyte immune response in the separately immunized mice. We observed low variations among the three fusion proteins in terms of the antibody and cytokine production in mice. Hence, in this study, results demonstrated that the structural proteins of SAT2 FMDV could be used for the development of immunodiagnostic kits and subunit vaccine designs.
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spelling pubmed-82271942021-06-26 Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus Li, Guoxiu Wubshet, Ashenafi Kiros Ding, Yaozhong Li, Qian Dai, Junfei Wang, Yang Hou, Qian Chen, Jiao Ma, Bing Szczotka-Bochniarz, Anna Szathmary, Susan Zhang, Yongguang Zhang, Jie Viruses Article An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV). However, the distinct antigenicity and immunogenicity of VP1, VP0, and VP3 of FMDV serotype SAT2 are poorly understood. Similarly, the particular roles of the three structural proteins in novel vaccine design and development remain unexplained. We therefore constructed VP1, VP0, and VP3 encoding gene (SAT2:JX014256 strain) separately fused with His-SUMO (histidine-small ubiquitin-related modifier) inserted into pET-32a cassette to express the three recombinant proteins and separately evaluated their antigenicity and immunogenicity in mice. The fusion protein was successfully expressed and purified by the Ni-NTA resin chromatography. The level of serum antibody, spleen lymphocyte proliferation, and cytokines against the three distinct recombinant proteins were analyzed. Results showed that the anti-FMDV humoral response was triggered by these proteins, and the fusion proteins did enhance the splenocyte immune response in the separately immunized mice. We observed low variations among the three fusion proteins in terms of the antibody and cytokine production in mice. Hence, in this study, results demonstrated that the structural proteins of SAT2 FMDV could be used for the development of immunodiagnostic kits and subunit vaccine designs. MDPI 2021-05-27 /pmc/articles/PMC8227194/ /pubmed/34072100 http://dx.doi.org/10.3390/v13061005 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Guoxiu
Wubshet, Ashenafi Kiros
Ding, Yaozhong
Li, Qian
Dai, Junfei
Wang, Yang
Hou, Qian
Chen, Jiao
Ma, Bing
Szczotka-Bochniarz, Anna
Szathmary, Susan
Zhang, Yongguang
Zhang, Jie
Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus
title Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus
title_full Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus
title_fullStr Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus
title_full_unstemmed Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus
title_short Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus
title_sort antigenicity and immunogenicity analysis of the e. coli expressed fmdv structural proteins; vp1, vp0, vp3 of the south african territories type 2 virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227194/
https://www.ncbi.nlm.nih.gov/pubmed/34072100
http://dx.doi.org/10.3390/v13061005
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