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Benchmarking DNA Extraction Methods for Phylogenomic Analysis of Sub-Antarctic Rhodococcus and Williamsia Species
Bacteria containing mycolic acids in their cell envelope are often recalcitrant to cell lysis, so extracting DNA of sufficient quality for third-generation sequencing and high-fidelity genome assembly requires optimization, even when using commercial kits with protocols for hard-to-lyse bacteria. We...
Autores principales: | Nahar, Akhikun, Baker, Anthony L., Nichols, David S., Bowman, John P., Britz, Margaret L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227252/ https://www.ncbi.nlm.nih.gov/pubmed/34207615 http://dx.doi.org/10.3390/microorganisms9061253 |
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