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Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System

Vascular and traumatic injuries of the central nervous system are recognized as global health priorities. A polypharmacology approach that is able to simultaneously target several injury factors by the combination of agents having synergistic effects appears to be promising. Herein, we designed a po...

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Autores principales: Dolci, Luisa Stella, Perone, Rosaria Carmela, Di Gesù, Roberto, Kurakula, Mallesh, Gualandi, Chiara, Zironi, Elisa, Gazzotti, Teresa, Tondo, Maria Teresa, Pagliuca, Giampiero, Gostynska, Natalia, Baldassarro, Vito Antonio, Cescatti, Maura, Giardino, Luciana, Focarete, Maria Letizia, Calzà, Laura, Passerini, Nadia, Bolognesi, Maria Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227370/
https://www.ncbi.nlm.nih.gov/pubmed/34201089
http://dx.doi.org/10.3390/pharmaceutics13060848
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author Dolci, Luisa Stella
Perone, Rosaria Carmela
Di Gesù, Roberto
Kurakula, Mallesh
Gualandi, Chiara
Zironi, Elisa
Gazzotti, Teresa
Tondo, Maria Teresa
Pagliuca, Giampiero
Gostynska, Natalia
Baldassarro, Vito Antonio
Cescatti, Maura
Giardino, Luciana
Focarete, Maria Letizia
Calzà, Laura
Passerini, Nadia
Bolognesi, Maria Laura
author_facet Dolci, Luisa Stella
Perone, Rosaria Carmela
Di Gesù, Roberto
Kurakula, Mallesh
Gualandi, Chiara
Zironi, Elisa
Gazzotti, Teresa
Tondo, Maria Teresa
Pagliuca, Giampiero
Gostynska, Natalia
Baldassarro, Vito Antonio
Cescatti, Maura
Giardino, Luciana
Focarete, Maria Letizia
Calzà, Laura
Passerini, Nadia
Bolognesi, Maria Laura
author_sort Dolci, Luisa Stella
collection PubMed
description Vascular and traumatic injuries of the central nervous system are recognized as global health priorities. A polypharmacology approach that is able to simultaneously target several injury factors by the combination of agents having synergistic effects appears to be promising. Herein, we designed a polymeric delivery system loaded with two drugs, ibuprofen (Ibu) and thyroid hormone triiodothyronine (T3) to in vitro release the suitable amount of the anti-inflammation and the remyelination drug. As a production method, electrospinning technology was used. First, Ibu-loaded micro (diameter circa 0.95–1.20 µm) and nano (diameter circa 0.70 µm) fibers were produced using poly(l-lactide) PLLA and PLGA with different lactide/glycolide ratios (50:50, 75:25, and 85:15) to select the most suitable polymer and fiber diameter. Based on the in vitro release results and in-house knowledge, PLLA nanofibers (mean diameter = 580 ± 120 nm) loaded with both Ibu and T3 were then successfully produced by a co-axial electrospinning technique. The in vitro release studies demonstrated that the final Ibu/T3 PLLA system extended the release of both drugs for 14 days, providing the target sustained release. Finally, studies in cell cultures (RAW macrophages and neural stem cell-derived oligodendrocyte precursor cells—OPCs) demonstrated the anti-inflammatory and promyelinating efficacy of the dual drug-loaded delivery platform.
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spelling pubmed-82273702021-06-26 Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System Dolci, Luisa Stella Perone, Rosaria Carmela Di Gesù, Roberto Kurakula, Mallesh Gualandi, Chiara Zironi, Elisa Gazzotti, Teresa Tondo, Maria Teresa Pagliuca, Giampiero Gostynska, Natalia Baldassarro, Vito Antonio Cescatti, Maura Giardino, Luciana Focarete, Maria Letizia Calzà, Laura Passerini, Nadia Bolognesi, Maria Laura Pharmaceutics Article Vascular and traumatic injuries of the central nervous system are recognized as global health priorities. A polypharmacology approach that is able to simultaneously target several injury factors by the combination of agents having synergistic effects appears to be promising. Herein, we designed a polymeric delivery system loaded with two drugs, ibuprofen (Ibu) and thyroid hormone triiodothyronine (T3) to in vitro release the suitable amount of the anti-inflammation and the remyelination drug. As a production method, electrospinning technology was used. First, Ibu-loaded micro (diameter circa 0.95–1.20 µm) and nano (diameter circa 0.70 µm) fibers were produced using poly(l-lactide) PLLA and PLGA with different lactide/glycolide ratios (50:50, 75:25, and 85:15) to select the most suitable polymer and fiber diameter. Based on the in vitro release results and in-house knowledge, PLLA nanofibers (mean diameter = 580 ± 120 nm) loaded with both Ibu and T3 were then successfully produced by a co-axial electrospinning technique. The in vitro release studies demonstrated that the final Ibu/T3 PLLA system extended the release of both drugs for 14 days, providing the target sustained release. Finally, studies in cell cultures (RAW macrophages and neural stem cell-derived oligodendrocyte precursor cells—OPCs) demonstrated the anti-inflammatory and promyelinating efficacy of the dual drug-loaded delivery platform. MDPI 2021-06-08 /pmc/articles/PMC8227370/ /pubmed/34201089 http://dx.doi.org/10.3390/pharmaceutics13060848 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dolci, Luisa Stella
Perone, Rosaria Carmela
Di Gesù, Roberto
Kurakula, Mallesh
Gualandi, Chiara
Zironi, Elisa
Gazzotti, Teresa
Tondo, Maria Teresa
Pagliuca, Giampiero
Gostynska, Natalia
Baldassarro, Vito Antonio
Cescatti, Maura
Giardino, Luciana
Focarete, Maria Letizia
Calzà, Laura
Passerini, Nadia
Bolognesi, Maria Laura
Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System
title Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System
title_full Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System
title_fullStr Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System
title_full_unstemmed Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System
title_short Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System
title_sort design and in vitro study of a dual drug-loaded delivery system produced by electrospinning for the treatment of acute injuries of the central nervous system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227370/
https://www.ncbi.nlm.nih.gov/pubmed/34201089
http://dx.doi.org/10.3390/pharmaceutics13060848
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