Cargando…
Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System
Vascular and traumatic injuries of the central nervous system are recognized as global health priorities. A polypharmacology approach that is able to simultaneously target several injury factors by the combination of agents having synergistic effects appears to be promising. Herein, we designed a po...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227370/ https://www.ncbi.nlm.nih.gov/pubmed/34201089 http://dx.doi.org/10.3390/pharmaceutics13060848 |
_version_ | 1783712507791147008 |
---|---|
author | Dolci, Luisa Stella Perone, Rosaria Carmela Di Gesù, Roberto Kurakula, Mallesh Gualandi, Chiara Zironi, Elisa Gazzotti, Teresa Tondo, Maria Teresa Pagliuca, Giampiero Gostynska, Natalia Baldassarro, Vito Antonio Cescatti, Maura Giardino, Luciana Focarete, Maria Letizia Calzà, Laura Passerini, Nadia Bolognesi, Maria Laura |
author_facet | Dolci, Luisa Stella Perone, Rosaria Carmela Di Gesù, Roberto Kurakula, Mallesh Gualandi, Chiara Zironi, Elisa Gazzotti, Teresa Tondo, Maria Teresa Pagliuca, Giampiero Gostynska, Natalia Baldassarro, Vito Antonio Cescatti, Maura Giardino, Luciana Focarete, Maria Letizia Calzà, Laura Passerini, Nadia Bolognesi, Maria Laura |
author_sort | Dolci, Luisa Stella |
collection | PubMed |
description | Vascular and traumatic injuries of the central nervous system are recognized as global health priorities. A polypharmacology approach that is able to simultaneously target several injury factors by the combination of agents having synergistic effects appears to be promising. Herein, we designed a polymeric delivery system loaded with two drugs, ibuprofen (Ibu) and thyroid hormone triiodothyronine (T3) to in vitro release the suitable amount of the anti-inflammation and the remyelination drug. As a production method, electrospinning technology was used. First, Ibu-loaded micro (diameter circa 0.95–1.20 µm) and nano (diameter circa 0.70 µm) fibers were produced using poly(l-lactide) PLLA and PLGA with different lactide/glycolide ratios (50:50, 75:25, and 85:15) to select the most suitable polymer and fiber diameter. Based on the in vitro release results and in-house knowledge, PLLA nanofibers (mean diameter = 580 ± 120 nm) loaded with both Ibu and T3 were then successfully produced by a co-axial electrospinning technique. The in vitro release studies demonstrated that the final Ibu/T3 PLLA system extended the release of both drugs for 14 days, providing the target sustained release. Finally, studies in cell cultures (RAW macrophages and neural stem cell-derived oligodendrocyte precursor cells—OPCs) demonstrated the anti-inflammatory and promyelinating efficacy of the dual drug-loaded delivery platform. |
format | Online Article Text |
id | pubmed-8227370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82273702021-06-26 Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System Dolci, Luisa Stella Perone, Rosaria Carmela Di Gesù, Roberto Kurakula, Mallesh Gualandi, Chiara Zironi, Elisa Gazzotti, Teresa Tondo, Maria Teresa Pagliuca, Giampiero Gostynska, Natalia Baldassarro, Vito Antonio Cescatti, Maura Giardino, Luciana Focarete, Maria Letizia Calzà, Laura Passerini, Nadia Bolognesi, Maria Laura Pharmaceutics Article Vascular and traumatic injuries of the central nervous system are recognized as global health priorities. A polypharmacology approach that is able to simultaneously target several injury factors by the combination of agents having synergistic effects appears to be promising. Herein, we designed a polymeric delivery system loaded with two drugs, ibuprofen (Ibu) and thyroid hormone triiodothyronine (T3) to in vitro release the suitable amount of the anti-inflammation and the remyelination drug. As a production method, electrospinning technology was used. First, Ibu-loaded micro (diameter circa 0.95–1.20 µm) and nano (diameter circa 0.70 µm) fibers were produced using poly(l-lactide) PLLA and PLGA with different lactide/glycolide ratios (50:50, 75:25, and 85:15) to select the most suitable polymer and fiber diameter. Based on the in vitro release results and in-house knowledge, PLLA nanofibers (mean diameter = 580 ± 120 nm) loaded with both Ibu and T3 were then successfully produced by a co-axial electrospinning technique. The in vitro release studies demonstrated that the final Ibu/T3 PLLA system extended the release of both drugs for 14 days, providing the target sustained release. Finally, studies in cell cultures (RAW macrophages and neural stem cell-derived oligodendrocyte precursor cells—OPCs) demonstrated the anti-inflammatory and promyelinating efficacy of the dual drug-loaded delivery platform. MDPI 2021-06-08 /pmc/articles/PMC8227370/ /pubmed/34201089 http://dx.doi.org/10.3390/pharmaceutics13060848 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dolci, Luisa Stella Perone, Rosaria Carmela Di Gesù, Roberto Kurakula, Mallesh Gualandi, Chiara Zironi, Elisa Gazzotti, Teresa Tondo, Maria Teresa Pagliuca, Giampiero Gostynska, Natalia Baldassarro, Vito Antonio Cescatti, Maura Giardino, Luciana Focarete, Maria Letizia Calzà, Laura Passerini, Nadia Bolognesi, Maria Laura Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System |
title | Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System |
title_full | Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System |
title_fullStr | Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System |
title_full_unstemmed | Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System |
title_short | Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous System |
title_sort | design and in vitro study of a dual drug-loaded delivery system produced by electrospinning for the treatment of acute injuries of the central nervous system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227370/ https://www.ncbi.nlm.nih.gov/pubmed/34201089 http://dx.doi.org/10.3390/pharmaceutics13060848 |
work_keys_str_mv | AT dolciluisastella designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT peronerosariacarmela designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT digesuroberto designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT kurakulamallesh designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT gualandichiara designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT zironielisa designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT gazzottiteresa designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT tondomariateresa designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT pagliucagiampiero designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT gostynskanatalia designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT baldassarrovitoantonio designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT cescattimaura designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT giardinoluciana designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT focaretemarialetizia designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT calzalaura designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT passerininadia designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem AT bolognesimarialaura designandinvitrostudyofadualdrugloadeddeliverysystemproducedbyelectrospinningforthetreatmentofacuteinjuriesofthecentralnervoussystem |