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Contact Guidance Effect and Prevention of Microfouling on a Beta Titanium Alloy Surface Structured by Electron-Beam Technology

Nano- and micro-structuring of implantable materials constitute a promising approach to introduce mechanical contact guidance effect, drive cells colonization, as well as to prevent bacteria adhesion and biofilm aggregation, through antifouling topography. Accordingly, this paper aims to extend the...

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Autores principales: Ferraris, Sara, Warchomicka, Fernando, Barberi, Jacopo, Cochis, Andrea, Scalia, Alessandro Calogero, Spriano, Silvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227382/
https://www.ncbi.nlm.nih.gov/pubmed/34199432
http://dx.doi.org/10.3390/nano11061474
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author Ferraris, Sara
Warchomicka, Fernando
Barberi, Jacopo
Cochis, Andrea
Scalia, Alessandro Calogero
Spriano, Silvia
author_facet Ferraris, Sara
Warchomicka, Fernando
Barberi, Jacopo
Cochis, Andrea
Scalia, Alessandro Calogero
Spriano, Silvia
author_sort Ferraris, Sara
collection PubMed
description Nano- and micro-structuring of implantable materials constitute a promising approach to introduce mechanical contact guidance effect, drive cells colonization, as well as to prevent bacteria adhesion and biofilm aggregation, through antifouling topography. Accordingly, this paper aims to extend the application of e-beam surface texturing and nano-structuring to the beta titanium alloys, which are of great interest for biomedical implants because of the low Young modulus and the reduction of the stress shielding effect. The paper shows that surface texturing on the micro-scale (micro-grooves) is functional to a contact guidance effect on gingival fibroblasts. Moreover, nano-structuring, derived from the e-beam surface treatment, is effective to prevent microfouling. In fact, human fibroblasts were cultivated directly onto grooved specimens showing to sense the surface micro-structure thus spreading following the grooves’ orientation. Moreover, Staphylococcus aureus colonies adhesion was prevented by the nano-topographies in comparison to the mirror-polished control, thus demonstrating promising antifouling properties. Furthermore, the research goes into detail to understand the mechanism of microfouling prevention due to nano-topography and microstructure.
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spelling pubmed-82273822021-06-26 Contact Guidance Effect and Prevention of Microfouling on a Beta Titanium Alloy Surface Structured by Electron-Beam Technology Ferraris, Sara Warchomicka, Fernando Barberi, Jacopo Cochis, Andrea Scalia, Alessandro Calogero Spriano, Silvia Nanomaterials (Basel) Article Nano- and micro-structuring of implantable materials constitute a promising approach to introduce mechanical contact guidance effect, drive cells colonization, as well as to prevent bacteria adhesion and biofilm aggregation, through antifouling topography. Accordingly, this paper aims to extend the application of e-beam surface texturing and nano-structuring to the beta titanium alloys, which are of great interest for biomedical implants because of the low Young modulus and the reduction of the stress shielding effect. The paper shows that surface texturing on the micro-scale (micro-grooves) is functional to a contact guidance effect on gingival fibroblasts. Moreover, nano-structuring, derived from the e-beam surface treatment, is effective to prevent microfouling. In fact, human fibroblasts were cultivated directly onto grooved specimens showing to sense the surface micro-structure thus spreading following the grooves’ orientation. Moreover, Staphylococcus aureus colonies adhesion was prevented by the nano-topographies in comparison to the mirror-polished control, thus demonstrating promising antifouling properties. Furthermore, the research goes into detail to understand the mechanism of microfouling prevention due to nano-topography and microstructure. MDPI 2021-06-02 /pmc/articles/PMC8227382/ /pubmed/34199432 http://dx.doi.org/10.3390/nano11061474 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ferraris, Sara
Warchomicka, Fernando
Barberi, Jacopo
Cochis, Andrea
Scalia, Alessandro Calogero
Spriano, Silvia
Contact Guidance Effect and Prevention of Microfouling on a Beta Titanium Alloy Surface Structured by Electron-Beam Technology
title Contact Guidance Effect and Prevention of Microfouling on a Beta Titanium Alloy Surface Structured by Electron-Beam Technology
title_full Contact Guidance Effect and Prevention of Microfouling on a Beta Titanium Alloy Surface Structured by Electron-Beam Technology
title_fullStr Contact Guidance Effect and Prevention of Microfouling on a Beta Titanium Alloy Surface Structured by Electron-Beam Technology
title_full_unstemmed Contact Guidance Effect and Prevention of Microfouling on a Beta Titanium Alloy Surface Structured by Electron-Beam Technology
title_short Contact Guidance Effect and Prevention of Microfouling on a Beta Titanium Alloy Surface Structured by Electron-Beam Technology
title_sort contact guidance effect and prevention of microfouling on a beta titanium alloy surface structured by electron-beam technology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227382/
https://www.ncbi.nlm.nih.gov/pubmed/34199432
http://dx.doi.org/10.3390/nano11061474
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