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VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells

SIMPLE SUMMARY: Inorganic polyphosphate, a linear polymer of orthophosphoric acid, plays an important role in microorganisms’ stress resistance. Vtc4 protein synthetizes inorganic polyphosphate in yeast. Here we show that yeast lacking this protein exhibit very low polyphosphate level, decreased res...

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Autores principales: Tomashevsky, Alexander, Kulakovskaya, Ekaterina, Trilisenko, Ludmila, Kulakovskiy, Ivan V., Kulakovskaya, Tatiana, Fedorov, Alexey, Eldarov, Mikhail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227513/
https://www.ncbi.nlm.nih.gov/pubmed/34070801
http://dx.doi.org/10.3390/biology10060487
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author Tomashevsky, Alexander
Kulakovskaya, Ekaterina
Trilisenko, Ludmila
Kulakovskiy, Ivan V.
Kulakovskaya, Tatiana
Fedorov, Alexey
Eldarov, Mikhail
author_facet Tomashevsky, Alexander
Kulakovskaya, Ekaterina
Trilisenko, Ludmila
Kulakovskiy, Ivan V.
Kulakovskaya, Tatiana
Fedorov, Alexey
Eldarov, Mikhail
author_sort Tomashevsky, Alexander
collection PubMed
description SIMPLE SUMMARY: Inorganic polyphosphate, a linear polymer of orthophosphoric acid, plays an important role in microorganisms’ stress resistance. Vtc4 protein synthetizes inorganic polyphosphate in yeast. Here we show that yeast lacking this protein exhibit very low polyphosphate level, decreased resistance to alkaline stress, but increased resistance to oxidative and heavy metal stresses. We suggest the increased stress resistance is achieved by constitutive up-regulation of oxidative stress-response genes and decreased expression of Pho84 that is responsible for manganese uptake. ABSTRACT: Inorganic polyphosphate (polyP) is an important factor of alkaline, heavy metal, and oxidative stress resistance in microbial cells. In yeast, polyP is synthesized by Vtc4, a subunit of the vacuole transporter chaperone complex. Here, we report reduced but reliably detectable amounts of acid-soluble and acid-insoluble polyPs in the Δvtc4 strain of Saccharomyces cerevisiae, reaching 10% and 20% of the respective levels of the wild-type strain. The Δvtc4 strain has decreased resistance to alkaline stress but, unexpectedly, increased resistance to oxidation and heavy metal excess. We suggest that increased resistance is achieved through elevated expression of DDR2, which is implicated in stress response, and reduced expression of PHO84 encoding a phosphate and divalent metal transporter. The decreased Mg(2+)-dependent phosphate accumulation in Δvtc4 cells is consistent with reduced expression of PHO84. We discuss a possible role that polyP level plays in cellular signaling of stress response mobilization in yeast.
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spelling pubmed-82275132021-06-26 VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells Tomashevsky, Alexander Kulakovskaya, Ekaterina Trilisenko, Ludmila Kulakovskiy, Ivan V. Kulakovskaya, Tatiana Fedorov, Alexey Eldarov, Mikhail Biology (Basel) Article SIMPLE SUMMARY: Inorganic polyphosphate, a linear polymer of orthophosphoric acid, plays an important role in microorganisms’ stress resistance. Vtc4 protein synthetizes inorganic polyphosphate in yeast. Here we show that yeast lacking this protein exhibit very low polyphosphate level, decreased resistance to alkaline stress, but increased resistance to oxidative and heavy metal stresses. We suggest the increased stress resistance is achieved by constitutive up-regulation of oxidative stress-response genes and decreased expression of Pho84 that is responsible for manganese uptake. ABSTRACT: Inorganic polyphosphate (polyP) is an important factor of alkaline, heavy metal, and oxidative stress resistance in microbial cells. In yeast, polyP is synthesized by Vtc4, a subunit of the vacuole transporter chaperone complex. Here, we report reduced but reliably detectable amounts of acid-soluble and acid-insoluble polyPs in the Δvtc4 strain of Saccharomyces cerevisiae, reaching 10% and 20% of the respective levels of the wild-type strain. The Δvtc4 strain has decreased resistance to alkaline stress but, unexpectedly, increased resistance to oxidation and heavy metal excess. We suggest that increased resistance is achieved through elevated expression of DDR2, which is implicated in stress response, and reduced expression of PHO84 encoding a phosphate and divalent metal transporter. The decreased Mg(2+)-dependent phosphate accumulation in Δvtc4 cells is consistent with reduced expression of PHO84. We discuss a possible role that polyP level plays in cellular signaling of stress response mobilization in yeast. MDPI 2021-05-30 /pmc/articles/PMC8227513/ /pubmed/34070801 http://dx.doi.org/10.3390/biology10060487 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tomashevsky, Alexander
Kulakovskaya, Ekaterina
Trilisenko, Ludmila
Kulakovskiy, Ivan V.
Kulakovskaya, Tatiana
Fedorov, Alexey
Eldarov, Mikhail
VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells
title VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells
title_full VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells
title_fullStr VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells
title_full_unstemmed VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells
title_short VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells
title_sort vtc4 polyphosphate polymerase knockout increases stress resistance of saccharomyces cerevisiae cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227513/
https://www.ncbi.nlm.nih.gov/pubmed/34070801
http://dx.doi.org/10.3390/biology10060487
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