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Protective Effects of Fucoxanthin on Hydrogen Peroxide-Induced Calcification of Heart Valve Interstitial Cells
Cardiovascular diseases such as atherosclerosis and aortic valve sclerosis involve inflammatory reactions triggered by various stimuli, causing increased oxidative stress. This increased oxidative stress causes damage to the heart cells, with subsequent cell apoptosis or calcification. Currently, he...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227531/ https://www.ncbi.nlm.nih.gov/pubmed/34073219 http://dx.doi.org/10.3390/md19060307 |
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author | Chiang, Yi-Fen Tsai, Chih-Hung Chen, Hsin-Yuan Wang, Kai-Lee Chang, Hsin-Yi Huang, Yun-Ju Hong, Yong-Han Ali, Mohamed Shieh, Tzong-Ming Huang, Tsui-Chin Lin, Ching-I Hsia, Shih-Min |
author_facet | Chiang, Yi-Fen Tsai, Chih-Hung Chen, Hsin-Yuan Wang, Kai-Lee Chang, Hsin-Yi Huang, Yun-Ju Hong, Yong-Han Ali, Mohamed Shieh, Tzong-Ming Huang, Tsui-Chin Lin, Ching-I Hsia, Shih-Min |
author_sort | Chiang, Yi-Fen |
collection | PubMed |
description | Cardiovascular diseases such as atherosclerosis and aortic valve sclerosis involve inflammatory reactions triggered by various stimuli, causing increased oxidative stress. This increased oxidative stress causes damage to the heart cells, with subsequent cell apoptosis or calcification. Currently, heart valve damage or heart valve diseases are treated by drugs or surgery. Natural antioxidant products are being investigated in related research, such as fucoxanthin (Fx), which is a marine carotenoid extracted from seaweed, with strong antioxidant, anti-inflammatory, and anti-tumor properties. This study aimed to explore the protective effect of Fx on heart valves under high oxidative stress, as well as the underlying mechanism of action. Rat heart valve interstitial cells under H(2)O(2)-induced oxidative stress were treated with Fx. Fx improved cell survival and reduced oxidative stress-induced DNA damage, which was assessed by cell viability analysis and staining with propidium iodide. Alizarin Red-S analysis indicated that Fx has a protective effect against calcification. Furthermore, Western blotting revealed that Fx abrogates oxidative stress-induced apoptosis via reducing the expression of apoptosis-related proteins as well as modulate Akt/ERK-related protein expression. Notably, in vivo experiments using 26 dogs treated with 60 mg/kg of Fx in combination with medical treatment for 0.5 to 2 years showed significant recovery in their echocardiographic parameters. Collectively, these in vitro and in vivo results highlight the potential of Fx to protect heart valve cells from high oxidative stress-induced damage. |
format | Online Article Text |
id | pubmed-8227531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82275312021-06-26 Protective Effects of Fucoxanthin on Hydrogen Peroxide-Induced Calcification of Heart Valve Interstitial Cells Chiang, Yi-Fen Tsai, Chih-Hung Chen, Hsin-Yuan Wang, Kai-Lee Chang, Hsin-Yi Huang, Yun-Ju Hong, Yong-Han Ali, Mohamed Shieh, Tzong-Ming Huang, Tsui-Chin Lin, Ching-I Hsia, Shih-Min Mar Drugs Article Cardiovascular diseases such as atherosclerosis and aortic valve sclerosis involve inflammatory reactions triggered by various stimuli, causing increased oxidative stress. This increased oxidative stress causes damage to the heart cells, with subsequent cell apoptosis or calcification. Currently, heart valve damage or heart valve diseases are treated by drugs or surgery. Natural antioxidant products are being investigated in related research, such as fucoxanthin (Fx), which is a marine carotenoid extracted from seaweed, with strong antioxidant, anti-inflammatory, and anti-tumor properties. This study aimed to explore the protective effect of Fx on heart valves under high oxidative stress, as well as the underlying mechanism of action. Rat heart valve interstitial cells under H(2)O(2)-induced oxidative stress were treated with Fx. Fx improved cell survival and reduced oxidative stress-induced DNA damage, which was assessed by cell viability analysis and staining with propidium iodide. Alizarin Red-S analysis indicated that Fx has a protective effect against calcification. Furthermore, Western blotting revealed that Fx abrogates oxidative stress-induced apoptosis via reducing the expression of apoptosis-related proteins as well as modulate Akt/ERK-related protein expression. Notably, in vivo experiments using 26 dogs treated with 60 mg/kg of Fx in combination with medical treatment for 0.5 to 2 years showed significant recovery in their echocardiographic parameters. Collectively, these in vitro and in vivo results highlight the potential of Fx to protect heart valve cells from high oxidative stress-induced damage. MDPI 2021-05-26 /pmc/articles/PMC8227531/ /pubmed/34073219 http://dx.doi.org/10.3390/md19060307 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chiang, Yi-Fen Tsai, Chih-Hung Chen, Hsin-Yuan Wang, Kai-Lee Chang, Hsin-Yi Huang, Yun-Ju Hong, Yong-Han Ali, Mohamed Shieh, Tzong-Ming Huang, Tsui-Chin Lin, Ching-I Hsia, Shih-Min Protective Effects of Fucoxanthin on Hydrogen Peroxide-Induced Calcification of Heart Valve Interstitial Cells |
title | Protective Effects of Fucoxanthin on Hydrogen Peroxide-Induced Calcification of Heart Valve Interstitial Cells |
title_full | Protective Effects of Fucoxanthin on Hydrogen Peroxide-Induced Calcification of Heart Valve Interstitial Cells |
title_fullStr | Protective Effects of Fucoxanthin on Hydrogen Peroxide-Induced Calcification of Heart Valve Interstitial Cells |
title_full_unstemmed | Protective Effects of Fucoxanthin on Hydrogen Peroxide-Induced Calcification of Heart Valve Interstitial Cells |
title_short | Protective Effects of Fucoxanthin on Hydrogen Peroxide-Induced Calcification of Heart Valve Interstitial Cells |
title_sort | protective effects of fucoxanthin on hydrogen peroxide-induced calcification of heart valve interstitial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227531/ https://www.ncbi.nlm.nih.gov/pubmed/34073219 http://dx.doi.org/10.3390/md19060307 |
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