Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase

[Image: see text] Deamination of cytosine and dUMP misincorporation have been found to be capable of producing uracil in the genome. This study presents the AI-seq (artificial incorporation modified nucleobase for sequencing), a “base substitution”, which not only is capable of profiling uracil at s...

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Autores principales: Wang, Yafen, Zhang, Xiong, Han, Shaoqing, Yang, Wei, Chen, Zonggui, Wu, Fan, Liu, Jizhou, Weng, Xiaocheng, Zhou, Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227591/
https://www.ncbi.nlm.nih.gov/pubmed/34235258
http://dx.doi.org/10.1021/acscentsci.0c01504
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author Wang, Yafen
Zhang, Xiong
Han, Shaoqing
Yang, Wei
Chen, Zonggui
Wu, Fan
Liu, Jizhou
Weng, Xiaocheng
Zhou, Xiang
author_facet Wang, Yafen
Zhang, Xiong
Han, Shaoqing
Yang, Wei
Chen, Zonggui
Wu, Fan
Liu, Jizhou
Weng, Xiaocheng
Zhou, Xiang
author_sort Wang, Yafen
collection PubMed
description [Image: see text] Deamination of cytosine and dUMP misincorporation have been found to be capable of producing uracil in the genome. This study presents the AI-seq (artificial incorporation modified nucleobase for sequencing), a “base substitution”, which not only is capable of profiling uracil at single-nucleotide resolution and showing its centromeric enrichment but could also reveal that the identified uracil sites are derived from cytosine deamination. All the results indicate the potential biological significance of uracil as the epigenetic modification.
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spelling pubmed-82275912021-07-06 Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase Wang, Yafen Zhang, Xiong Han, Shaoqing Yang, Wei Chen, Zonggui Wu, Fan Liu, Jizhou Weng, Xiaocheng Zhou, Xiang ACS Cent Sci [Image: see text] Deamination of cytosine and dUMP misincorporation have been found to be capable of producing uracil in the genome. This study presents the AI-seq (artificial incorporation modified nucleobase for sequencing), a “base substitution”, which not only is capable of profiling uracil at single-nucleotide resolution and showing its centromeric enrichment but could also reveal that the identified uracil sites are derived from cytosine deamination. All the results indicate the potential biological significance of uracil as the epigenetic modification. American Chemical Society 2021-04-28 2021-06-23 /pmc/articles/PMC8227591/ /pubmed/34235258 http://dx.doi.org/10.1021/acscentsci.0c01504 Text en © 2021 The Authors. Published by American Chemical Society Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Wang, Yafen
Zhang, Xiong
Han, Shaoqing
Yang, Wei
Chen, Zonggui
Wu, Fan
Liu, Jizhou
Weng, Xiaocheng
Zhou, Xiang
Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase
title Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase
title_full Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase
title_fullStr Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase
title_full_unstemmed Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase
title_short Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase
title_sort base-resolution analysis of deoxyuridine at the genome scale based on the artificial incorporation modified nucleobase
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227591/
https://www.ncbi.nlm.nih.gov/pubmed/34235258
http://dx.doi.org/10.1021/acscentsci.0c01504
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