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Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs
South Korea has experienced FMD outbreaks almost every year since 2014. Therefore, a novel local vaccine that can cover various topotypes of viruses is required. Two virus strains, O/Boeun/SKR/2017 and A/Yeoncheon/SKR/2017, were cultured up to the pilot scale based on the optimized conditions set up...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227705/ https://www.ncbi.nlm.nih.gov/pubmed/34199359 http://dx.doi.org/10.3390/vaccines9060586 |
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author | Park, Sang-Hyun Lee, Seo-Yong Kim, Jae-Seok Kim, Ah-Young Park, Sun-Young Lee, Ji-Hye Lee, Mijung Kim, Hyejin Lee, Sim-In Kang, Na-Young Park, Jung-Won Kim, Su-Mi Park, Jong-Hyeon Ko, Young-Joon |
author_facet | Park, Sang-Hyun Lee, Seo-Yong Kim, Jae-Seok Kim, Ah-Young Park, Sun-Young Lee, Ji-Hye Lee, Mijung Kim, Hyejin Lee, Sim-In Kang, Na-Young Park, Jung-Won Kim, Su-Mi Park, Jong-Hyeon Ko, Young-Joon |
author_sort | Park, Sang-Hyun |
collection | PubMed |
description | South Korea has experienced FMD outbreaks almost every year since 2014. Therefore, a novel local vaccine that can cover various topotypes of viruses is required. Two virus strains, O/Boeun/SKR/2017 and A/Yeoncheon/SKR/2017, were cultured up to the pilot scale based on the optimized conditions set up on the flask scale. FMDV particles (146S) of 2 µg/mL or more were obtained from the virus culture supernatant using a 100 L bioreactor. The viruses were fully inactivated using binary ethylenimine within 16 h through two inactivation cycles and mixed with an adjuvant into a bivalent vaccine (types O and A) consisting of 15 µg viruses per strain. The experimental bivalent vaccine showed a broad spectrum of high neutralizing antibody titers against heterologous viruses, including type O Cathay strain and type A Asia topotypes, except for GVII. The 50% protective dose was determined as 12.5 for O/Boeun/SKR/2017 and 15.6 for A/Yeoncheon/SKR/2017. Collectively, we expect that the bivalent vaccine could protect against FMDV types O and A circulating in South Korea and neighboring countries. To our knowledge, this is the first report demonstrating that the vaccine strains could be successfully scaled-up to a 100 L bioreactor, with the determination of its protective efficacy in pigs. |
format | Online Article Text |
id | pubmed-8227705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82277052021-06-26 Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs Park, Sang-Hyun Lee, Seo-Yong Kim, Jae-Seok Kim, Ah-Young Park, Sun-Young Lee, Ji-Hye Lee, Mijung Kim, Hyejin Lee, Sim-In Kang, Na-Young Park, Jung-Won Kim, Su-Mi Park, Jong-Hyeon Ko, Young-Joon Vaccines (Basel) Article South Korea has experienced FMD outbreaks almost every year since 2014. Therefore, a novel local vaccine that can cover various topotypes of viruses is required. Two virus strains, O/Boeun/SKR/2017 and A/Yeoncheon/SKR/2017, were cultured up to the pilot scale based on the optimized conditions set up on the flask scale. FMDV particles (146S) of 2 µg/mL or more were obtained from the virus culture supernatant using a 100 L bioreactor. The viruses were fully inactivated using binary ethylenimine within 16 h through two inactivation cycles and mixed with an adjuvant into a bivalent vaccine (types O and A) consisting of 15 µg viruses per strain. The experimental bivalent vaccine showed a broad spectrum of high neutralizing antibody titers against heterologous viruses, including type O Cathay strain and type A Asia topotypes, except for GVII. The 50% protective dose was determined as 12.5 for O/Boeun/SKR/2017 and 15.6 for A/Yeoncheon/SKR/2017. Collectively, we expect that the bivalent vaccine could protect against FMDV types O and A circulating in South Korea and neighboring countries. To our knowledge, this is the first report demonstrating that the vaccine strains could be successfully scaled-up to a 100 L bioreactor, with the determination of its protective efficacy in pigs. MDPI 2021-06-02 /pmc/articles/PMC8227705/ /pubmed/34199359 http://dx.doi.org/10.3390/vaccines9060586 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Park, Sang-Hyun Lee, Seo-Yong Kim, Jae-Seok Kim, Ah-Young Park, Sun-Young Lee, Ji-Hye Lee, Mijung Kim, Hyejin Lee, Sim-In Kang, Na-Young Park, Jung-Won Kim, Su-Mi Park, Jong-Hyeon Ko, Young-Joon Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs |
title | Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs |
title_full | Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs |
title_fullStr | Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs |
title_full_unstemmed | Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs |
title_short | Scale-Up Production of Type O and A Foot-and-Mouth Disease Bivalent Vaccine and Its Protective Efficacy in Pigs |
title_sort | scale-up production of type o and a foot-and-mouth disease bivalent vaccine and its protective efficacy in pigs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227705/ https://www.ncbi.nlm.nih.gov/pubmed/34199359 http://dx.doi.org/10.3390/vaccines9060586 |
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