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Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms

Nowadays, the intranasal route has become a reliable alternative route for drug administration to the systemic circulation or central nervous system. However, there are no official in vitro diffusion and dissolution tests especially for the investigation of nasal formulations. Our main goal was to s...

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Autores principales: Bartos, Csilla, Szabó-Révész, Piroska, Horváth, Tamás, Varga, Patrícia, Ambrus, Rita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227734/
https://www.ncbi.nlm.nih.gov/pubmed/34201053
http://dx.doi.org/10.3390/pharmaceutics13060846
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author Bartos, Csilla
Szabó-Révész, Piroska
Horváth, Tamás
Varga, Patrícia
Ambrus, Rita
author_facet Bartos, Csilla
Szabó-Révész, Piroska
Horváth, Tamás
Varga, Patrícia
Ambrus, Rita
author_sort Bartos, Csilla
collection PubMed
description Nowadays, the intranasal route has become a reliable alternative route for drug administration to the systemic circulation or central nervous system. However, there are no official in vitro diffusion and dissolution tests especially for the investigation of nasal formulations. Our main goal was to study and compare a well-known and a lesser-known in vitro permeability investigation method, in order to ascertain which was suitable for the determination of drug permeability through the nasal mucosa from different formulations. The vertical diffusion cell (Franz cell) was compared with the horizontal diffusion model (Side-Bi-Side). Raw and nanonized meloxicam containing nasal dosage forms (spray, gel and powder) were tested and compared. It was found that the Side-Bi-Side cell was suitable for the investigation of spray and powder forms. In contrast, the gel was not measurable on the Side-Bi-Side cell; due to its high viscosity, a uniform distribution of the active substance could not be ensured in the donor phase. The Franz cell, designed for the analysis of semi-solid formulations, was desirable for the investigation of nasal gels. It can be concluded that the application of a horizontal cell is recommended for liquid and solid nasal preparations, while the vertical one should be used for semi-solid formulations.
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spelling pubmed-82277342021-06-26 Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms Bartos, Csilla Szabó-Révész, Piroska Horváth, Tamás Varga, Patrícia Ambrus, Rita Pharmaceutics Article Nowadays, the intranasal route has become a reliable alternative route for drug administration to the systemic circulation or central nervous system. However, there are no official in vitro diffusion and dissolution tests especially for the investigation of nasal formulations. Our main goal was to study and compare a well-known and a lesser-known in vitro permeability investigation method, in order to ascertain which was suitable for the determination of drug permeability through the nasal mucosa from different formulations. The vertical diffusion cell (Franz cell) was compared with the horizontal diffusion model (Side-Bi-Side). Raw and nanonized meloxicam containing nasal dosage forms (spray, gel and powder) were tested and compared. It was found that the Side-Bi-Side cell was suitable for the investigation of spray and powder forms. In contrast, the gel was not measurable on the Side-Bi-Side cell; due to its high viscosity, a uniform distribution of the active substance could not be ensured in the donor phase. The Franz cell, designed for the analysis of semi-solid formulations, was desirable for the investigation of nasal gels. It can be concluded that the application of a horizontal cell is recommended for liquid and solid nasal preparations, while the vertical one should be used for semi-solid formulations. MDPI 2021-06-08 /pmc/articles/PMC8227734/ /pubmed/34201053 http://dx.doi.org/10.3390/pharmaceutics13060846 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bartos, Csilla
Szabó-Révész, Piroska
Horváth, Tamás
Varga, Patrícia
Ambrus, Rita
Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms
title Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms
title_full Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms
title_fullStr Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms
title_full_unstemmed Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms
title_short Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms
title_sort comparison of modern in vitro permeability methods with the aim of investigation nasal dosage forms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227734/
https://www.ncbi.nlm.nih.gov/pubmed/34201053
http://dx.doi.org/10.3390/pharmaceutics13060846
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