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Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms
Nowadays, the intranasal route has become a reliable alternative route for drug administration to the systemic circulation or central nervous system. However, there are no official in vitro diffusion and dissolution tests especially for the investigation of nasal formulations. Our main goal was to s...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227734/ https://www.ncbi.nlm.nih.gov/pubmed/34201053 http://dx.doi.org/10.3390/pharmaceutics13060846 |
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author | Bartos, Csilla Szabó-Révész, Piroska Horváth, Tamás Varga, Patrícia Ambrus, Rita |
author_facet | Bartos, Csilla Szabó-Révész, Piroska Horváth, Tamás Varga, Patrícia Ambrus, Rita |
author_sort | Bartos, Csilla |
collection | PubMed |
description | Nowadays, the intranasal route has become a reliable alternative route for drug administration to the systemic circulation or central nervous system. However, there are no official in vitro diffusion and dissolution tests especially for the investigation of nasal formulations. Our main goal was to study and compare a well-known and a lesser-known in vitro permeability investigation method, in order to ascertain which was suitable for the determination of drug permeability through the nasal mucosa from different formulations. The vertical diffusion cell (Franz cell) was compared with the horizontal diffusion model (Side-Bi-Side). Raw and nanonized meloxicam containing nasal dosage forms (spray, gel and powder) were tested and compared. It was found that the Side-Bi-Side cell was suitable for the investigation of spray and powder forms. In contrast, the gel was not measurable on the Side-Bi-Side cell; due to its high viscosity, a uniform distribution of the active substance could not be ensured in the donor phase. The Franz cell, designed for the analysis of semi-solid formulations, was desirable for the investigation of nasal gels. It can be concluded that the application of a horizontal cell is recommended for liquid and solid nasal preparations, while the vertical one should be used for semi-solid formulations. |
format | Online Article Text |
id | pubmed-8227734 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82277342021-06-26 Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms Bartos, Csilla Szabó-Révész, Piroska Horváth, Tamás Varga, Patrícia Ambrus, Rita Pharmaceutics Article Nowadays, the intranasal route has become a reliable alternative route for drug administration to the systemic circulation or central nervous system. However, there are no official in vitro diffusion and dissolution tests especially for the investigation of nasal formulations. Our main goal was to study and compare a well-known and a lesser-known in vitro permeability investigation method, in order to ascertain which was suitable for the determination of drug permeability through the nasal mucosa from different formulations. The vertical diffusion cell (Franz cell) was compared with the horizontal diffusion model (Side-Bi-Side). Raw and nanonized meloxicam containing nasal dosage forms (spray, gel and powder) were tested and compared. It was found that the Side-Bi-Side cell was suitable for the investigation of spray and powder forms. In contrast, the gel was not measurable on the Side-Bi-Side cell; due to its high viscosity, a uniform distribution of the active substance could not be ensured in the donor phase. The Franz cell, designed for the analysis of semi-solid formulations, was desirable for the investigation of nasal gels. It can be concluded that the application of a horizontal cell is recommended for liquid and solid nasal preparations, while the vertical one should be used for semi-solid formulations. MDPI 2021-06-08 /pmc/articles/PMC8227734/ /pubmed/34201053 http://dx.doi.org/10.3390/pharmaceutics13060846 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bartos, Csilla Szabó-Révész, Piroska Horváth, Tamás Varga, Patrícia Ambrus, Rita Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms |
title | Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms |
title_full | Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms |
title_fullStr | Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms |
title_full_unstemmed | Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms |
title_short | Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms |
title_sort | comparison of modern in vitro permeability methods with the aim of investigation nasal dosage forms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227734/ https://www.ncbi.nlm.nih.gov/pubmed/34201053 http://dx.doi.org/10.3390/pharmaceutics13060846 |
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