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Functional Characterization of the Photosynthetic Machinery in Smicronix Galls on the Parasitic Plant Cuscuta campestris by JIP-Test
Members of the genus Cuscuta are generally considered to be non-photosynthetic, stem-holoparasitic flowering plants. Under certain circumstances, at least some members of the genus are capable of limited photosynthesis. The galls of the Smicronyx weevils formed on Cuscuta campestris are particularly...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227743/ https://www.ncbi.nlm.nih.gov/pubmed/34198864 http://dx.doi.org/10.3390/cells10061399 |
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author | Zagorchev, Lyuben Atanasova, Alexandra Albanova, Ivanela Traianova, Anelia Mladenov, Petko Kouzmanova, Margarita Goltsev, Vasilij Kalaji, Hazem M. Teofanova, Denitsa |
author_facet | Zagorchev, Lyuben Atanasova, Alexandra Albanova, Ivanela Traianova, Anelia Mladenov, Petko Kouzmanova, Margarita Goltsev, Vasilij Kalaji, Hazem M. Teofanova, Denitsa |
author_sort | Zagorchev, Lyuben |
collection | PubMed |
description | Members of the genus Cuscuta are generally considered to be non-photosynthetic, stem-holoparasitic flowering plants. Under certain circumstances, at least some members of the genus are capable of limited photosynthesis. The galls of the Smicronyx weevils formed on Cuscuta campestris are particularly rich in chlorophylls compared to the stem of the parasitic plant. In the present study, we aimed to characterize the photosynthetic activity in the inner and outer gall cortices in comparison to the non-photosynthetic stems and a reference plant (Arabidopsis thaliana). The recorded prompt chlorophyll fluorescence transients were analyzed using JIP test. Detailed analysis of the chlorophyll fluorescence confirmed the presence of actively functioning photosynthetic machinery, especially in the inner cortex of the galls. This photosynthesis, induced by the insect larvae, did not reach the levels of the photosynthetic activity in Arabidopsis thaliana plants. Thylakoid protein complexes were identified by separation with two-dimensional Blue Native/SDS PAGE. It appeared that some of the complexes presented in A. thaliana are missing in C. campestris. We hypothesize that the insect-triggered transition from non-photosynthetic to photosynthetic tissue in the gall is driven by the increased requirements for nutrients related to the larval nutrition. |
format | Online Article Text |
id | pubmed-8227743 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82277432021-06-26 Functional Characterization of the Photosynthetic Machinery in Smicronix Galls on the Parasitic Plant Cuscuta campestris by JIP-Test Zagorchev, Lyuben Atanasova, Alexandra Albanova, Ivanela Traianova, Anelia Mladenov, Petko Kouzmanova, Margarita Goltsev, Vasilij Kalaji, Hazem M. Teofanova, Denitsa Cells Article Members of the genus Cuscuta are generally considered to be non-photosynthetic, stem-holoparasitic flowering plants. Under certain circumstances, at least some members of the genus are capable of limited photosynthesis. The galls of the Smicronyx weevils formed on Cuscuta campestris are particularly rich in chlorophylls compared to the stem of the parasitic plant. In the present study, we aimed to characterize the photosynthetic activity in the inner and outer gall cortices in comparison to the non-photosynthetic stems and a reference plant (Arabidopsis thaliana). The recorded prompt chlorophyll fluorescence transients were analyzed using JIP test. Detailed analysis of the chlorophyll fluorescence confirmed the presence of actively functioning photosynthetic machinery, especially in the inner cortex of the galls. This photosynthesis, induced by the insect larvae, did not reach the levels of the photosynthetic activity in Arabidopsis thaliana plants. Thylakoid protein complexes were identified by separation with two-dimensional Blue Native/SDS PAGE. It appeared that some of the complexes presented in A. thaliana are missing in C. campestris. We hypothesize that the insect-triggered transition from non-photosynthetic to photosynthetic tissue in the gall is driven by the increased requirements for nutrients related to the larval nutrition. MDPI 2021-06-05 /pmc/articles/PMC8227743/ /pubmed/34198864 http://dx.doi.org/10.3390/cells10061399 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zagorchev, Lyuben Atanasova, Alexandra Albanova, Ivanela Traianova, Anelia Mladenov, Petko Kouzmanova, Margarita Goltsev, Vasilij Kalaji, Hazem M. Teofanova, Denitsa Functional Characterization of the Photosynthetic Machinery in Smicronix Galls on the Parasitic Plant Cuscuta campestris by JIP-Test |
title | Functional Characterization of the Photosynthetic Machinery in Smicronix Galls on the Parasitic Plant Cuscuta campestris by JIP-Test |
title_full | Functional Characterization of the Photosynthetic Machinery in Smicronix Galls on the Parasitic Plant Cuscuta campestris by JIP-Test |
title_fullStr | Functional Characterization of the Photosynthetic Machinery in Smicronix Galls on the Parasitic Plant Cuscuta campestris by JIP-Test |
title_full_unstemmed | Functional Characterization of the Photosynthetic Machinery in Smicronix Galls on the Parasitic Plant Cuscuta campestris by JIP-Test |
title_short | Functional Characterization of the Photosynthetic Machinery in Smicronix Galls on the Parasitic Plant Cuscuta campestris by JIP-Test |
title_sort | functional characterization of the photosynthetic machinery in smicronix galls on the parasitic plant cuscuta campestris by jip-test |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227743/ https://www.ncbi.nlm.nih.gov/pubmed/34198864 http://dx.doi.org/10.3390/cells10061399 |
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